Restriction map for pF1KB7916
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AflII       AflIII      AhdI        Alw44I      AlwNI       
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvrII       BalI        BbeI        BbrPI       BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HgaI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         940  GACGTC               GACGT^C
AccI          769  GTAGAC               GT^[AC][GT]AC
AcyI          940  GACGTC               G[GA]^CG[CT]C
AgeI          735  ACCGGT               A^CCGGT
AocI          382  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       832  AGCGCT               AGC^GCT
ApaI          322  GGGCCC               GGGCC^C
AviII          23  TGCGCA               TGC^GCA
BamHI         164  GGATCC               G^GATCC
BanI         1013  GGTGCC               G^G[CT][GA]CC
BbsI          220  GAAGAC               GAAGAC(2/6)
BbvCI         543  CCTCAGC              CCTCAGC(-5/-2)
BcgI          174  CGAACTGCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI          204  CTGGAG               CTGGAG(16/14)
BpuAI         220  GAAGAC               GAAGAC(2/6)
BsaHI         940  GACGTC               G[GA]^CG[CT]C
BsaI          296  GAGACC               GGTCTC(1/5)
BsaWI         735  ACCGGT               [AT]^CCGG[AT]
BsmFI         634  GGGAC                GGGAC(10/14)
BsmI         1025  GCATTC               GAATGC(1/-1)
BsrFI         735  ACCGGT               [GA]^CCGG[CT]
BstYI         164  GGATCC               [GA]^GATC[CT]
Bsu36I        382  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        735  ACCGGT               [GA]^CCGG[CT]
DraIII        682  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          807  TGGCCG               [CT]^GGCC[GA]
EarI          904  GAAGAG               CTCTTC(1/4)
Eco47III      832  AGCGCT               AGC^GCT
Eco81I        382  CCTCAGG              CC^T[ACGT]AGG
EcoNI         894  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI           23  TGCGCA               TGC^GCA
HaeII         832  AGCGCT               [GA]GCGC^[CT]
Hin1I         940  GACGTC               G[GA]^CG[CT]C
Ksp632I       904  GAAGAG               CTCTTC(1/4)
MflI          164  GGATCC               [GA]^GATC[CT]
MspA1I        478  CAGCGG               C[AC]G^C[GT]G
NcoI          839  CCATGG               C^CATGG
NspI          180  GCATGC               [GA]CATG^[CT]
PinAI         735  ACCGGT               A^CCGGT
SphI          180  GCATGC               GCATG^C
TaqI          801  TCGA                 T^CGA
Tsp509I       284  AATT                 ^AATT
TthHB8I       801  TCGA                 T^CGA
XcmI          138  CCAGCAGGAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         164  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AluI          551  AGCT                 AG^CT
              879  AGCT                
AvaI          191  CCCGGG               C^[CT]CG[GA]G
              428  CCCGGG              
BfaI          416  CTAG                 C^TAG
              581  CTAG                
BsgI           84  CTGCAC               GTGCAG(16/14)
              875  GTGCAG              
BsmAI         296  GAGAC                GTCTC(1/5)
              907  GAGAC               
BsoBI         191  CCCGGG               C^[CT]CG[GA]G
              428  CCCGGG              
CfoI           24  GCGC                 GCG^C
              833  GCGC                
DraII         321  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              322  GGGCCCC             
DrdI          202  GACTGGAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              803  GACGTGGCCGTC        
DsaI          409  CCACGG               C^C[GA][CT]GG
              839  CCATGG              
EcoO109I      321  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              322  GGGCCCC             
HhaI           24  GCGC                 GCG^C
              833  GCGC                
HinP1I         24  GCGC                 G^CGC
              833  GCGC                
MaeI          416  CTAG                 C^TAG
              581  CTAG                
MaeII         804  ACGT                 A^CGT
              941  ACGT                
MaeIII        272  GTCAC                ^GT[ACGT]AC
              938  GTGAC               
PstI          851  CTGCAG               CTGCA^G
              969  CTGCAG              
SfaNI        1004  GATGC                GCATC(5/9)
              974  GCATC               
SfcI          851  CTGCAG               C^T[GA][CT]AG
              969  CTGCAG              
SmaI          191  CCCGGG               CCC^GGG
              428  CCCGGG              
Tsp45I        272  GTCAC                ^GT[GC]AC
              938  GTGAC               
XmaCI         191  CCCGGG               C^CCGGG
              428  CCCGGG              
XmaI          191  CCCGGG               C^CCGGG
              428  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AlwI        AvaII       BanII       
BbvI        BcnI        BglI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com