Restriction map for pF1KB7916
 (Linear) (Six-base) MAPSORT of: KIBB7916.seq  Check: 128  from: 1  to: 1035



KIBB7916.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AatII G_ACGT'C

Cuts at:      0     944    1035 
   Size:        944      91
 
AccI GT'mk_AC

Cuts at:      0     770    1035 
   Size:        770     265
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338    1035 
   Size:        338     697
 
AfeI AGC'GCT

Cuts at:      0     834    1035 
   Size:        834     201
 
AgeI A'CCGG_T

Cuts at:      0     735    1035 
   Size:        735     300
 
ApaI G_GGCC'C

Cuts at:      0     326    1035 
   Size:        326     709
 
AvaI C'yCGr_G

Cuts at:      0     191     428    1035 
   Size:        191     237     607

  Fragments arranged by size: 

                 607     237     191
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     230     263    1035 
   Size:        230      33     772

  Fragments arranged by size: 

                 772     230      33
 
BamHI G'GATC_C

Cuts at:      0     164    1035 
   Size:        164     871
 
BanI G'GyrC_C

Cuts at:      0    1013    1035 
   Size:       1013      22
 
BanII G_rGCy'C

Cuts at:      0     191     326     474     752     872    1035 
   Size:        191     135     148     278     120     163

  Fragments arranged by size: 

                 278     191     163     148     135     120
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     227    1035 
   Size:        227     808
 
BbvCI CC'TCA_GC

Cuts at:      0     544    1035 
   Size:        544     491
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     163     197    1035 
   Size:        163      34     838

  Fragments arranged by size: 

                 838     163      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     511     991    1022    1035 
   Size:        511     480      31      13

  Fragments arranged by size: 

                 511     480      31      13
 
Bme1580I G_kGCm'C

Cuts at:      0     326    1035 
   Size:        326     709
 
BmgBI CAC'GTC

Cuts at:      0     805    1035 
   Size:        805     230
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     820    1035 
   Size:        820     215
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     734    1035 
   Size:        734     301
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225    1035 
   Size:        225     810
 
Bpu10I CC'TnA_GC

Cuts at:      0     544    1035 
   Size:        544     491
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     290    1035 
   Size:        290     745
 
BsaHI Gr'CG_yC

Cuts at:      0     941    1035 
   Size:        941      94
 
BsaWI w'CCGG_w

Cuts at:      0     735    1035 
   Size:        735     300
 
BseYI C'CCAG_C

Cuts at:      0     137     560    1035 
   Size:        137     423     475

  Fragments arranged by size: 

                 475     423     137
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69     896    1035 
   Size:         69     827     139

  Fragments arranged by size: 

                 827     139      69
 
BsmI GAATG_Cn'

Cuts at:      0    1025    1035 
   Size:       1025      10
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     326     474     752     872    1035 
   Size:        191     135     148     278     120     163

  Fragments arranged by size: 

                 278     191     163     148     135     120
 
BsrFI r'CCGG_y

Cuts at:      0     735    1035 
   Size:        735     300
 
BstYI r'GATC_y

Cuts at:      0     164    1035 
   Size:        164     871
 
Bsu36I CC'TnA_GG

Cuts at:      0     383    1035 
   Size:        383     652
 
BtgI C'CryG_G

Cuts at:      0     409     839    1035 
   Size:        409     430     196

  Fragments arranged by size: 

                 430     409     196
 
DraIII CAC_nnn'GTG

Cuts at:      0     687    1035 
   Size:        687     348
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     208     809    1035 
   Size:        208     601     226

  Fragments arranged by size: 

                 601     226     208
 
EaeI y'GGCC_r

Cuts at:      0     807    1035 
   Size:        807     228
 
EarI CTCTTCn'nnn_

Cuts at:      0     899    1035 
   Size:        899     136
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     709    1035 
   Size:        709     326
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     338    1035 
   Size:        225     113     697

  Fragments arranged by size: 

                 697     225     113
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     898    1035 
   Size:        898     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     322     323    1035 
   Size:        322       1     712

  Fragments arranged by size: 

                 712     322       1
 
FspI TGC'GCA

Cuts at:      0      25    1035 
   Size:         25    1010
 
HaeII r_GCGC'y

Cuts at:      0     836    1035 
   Size:        836     199
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     282     387    1035 
   Size:         39     243     105     648

  Fragments arranged by size: 

                 648     243     105      39
 
MspA1I CmG'CkG

Cuts at:      0     480    1035 
   Size:        480     555
 
NcoI C'CATG_G

Cuts at:      0     839    1035 
   Size:        839     196
 
NspI r_CATG'y

Cuts at:      0     184    1035 
   Size:        184     851
 
PasI CC'CwG_GG

Cuts at:      0     465    1009    1035 
   Size:        465     544      26

  Fragments arranged by size: 

                 544     465      26
 
PspOMI G'GGCC_C

Cuts at:      0     322    1035 
   Size:        322     713
 
PstI C_TGCA'G

Cuts at:      0     855     973    1035 
   Size:        855     118      62

  Fragments arranged by size: 

                 855     118      62
 
SfcI C'TryA_G

Cuts at:      0     851     969    1035 
   Size:        851     118      66

  Fragments arranged by size: 

                 851     118      66
 
SmaI CCC'GGG

Cuts at:      0     193     430    1035 
   Size:        193     237     605

  Fragments arranged by size: 

                 605     237     193
 
SphI G_CATG'C

Cuts at:      0     184    1035 
   Size:        184     851
 
StyI C'CwwG_G

Cuts at:      0     234     509     839    1035 
   Size:        234     275     330     196

  Fragments arranged by size: 

                 330     275     234     196
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     145    1035 
   Size:        145     890
 
XmaI C'CCGG_G

Cuts at:      0     191     428    1035 
   Size:        191     237     607

  Fragments arranged by size: 

                 607     237     191
 
ZraI GAC'GTC

Cuts at:      0     942    1035 
   Size:        942      93
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI     AgeI     ApaI     AvaI     BaeI
    BamHI     BanI    BanII     BbsI    BbvCI     BcgI     BglI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI
    BseYI     BsgI     BsmI Bsp1286I    BsrFI    BstYI   Bsu36I     BtgI
   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FspI    HaeII     MmeI   MspA1I     NcoI     NspI     PasI   PspOMI
     PstI     SfcI     SmaI     SphI     StyI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BbeI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI    BpuEI
    BsaAI    BsaBI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmnI
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