Restriction map for pF1KB6912
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
DraI        DraII       DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        SacI        
SalI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII         169  GACGTC               GACGT^C
AflIII        396  ACACGT               A^C[GA][CT]GT
BanII         562  GAGCCC               G[GA]GC[CT]^C
BciVI         584  GGATAC               GTATCC(6/5)
BsaI          115  GAGACC               GGTCTC(1/5)
BsgI          577  GTGCAG               GTGCAG(16/14)
BsrBI         214  CCGCTC               CCGCTC(-3/-3)
BssHII         54  GCGCGC               G^CGCGC
BssSI         433  CACGAG               CACGAG(-5/-1)
BstF5I        348  CATCC                GGATG(2/0)
CpoI          152  CGGTCCG              CG^G[AT]CCG
DdeI          371  CTGAG                C^T[ACGT]AG
EarI          217  CTCTTC               CTCTTC(1/4)
EcoT14I       638  CCAAGG               C^C[AT][AT]GG
FokI          348  CATCC                GGATG(9/13)
HinfI          88  GACTC                G^A[ACGT]TC
HphI          449  TCACC                GGTGA(8/7)
Ksp632I       217  CTCTTC               CTCTTC(1/4)
KspI           22  CCGCGG               CCGC^GG
MaeIII        238  GTCAC                ^GT[ACGT]AC
MslI          291  CACGCGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI           88  GACTC                GAGTC(4/5)
PvuII         196  CAGCTG               CAG^CTG
RsrII         152  CGGTCCG              CG^G[AT]CCG
SacII          22  CCGCGG               CCGC^GG
SapI          216  GCTCTTC              GCTCTTC(1/4)
SfaNI         347  GCATC                GCATC(5/9)
SgrAI         461  CGCCGGCG             C[GA]^CCGG[CT]G
StyI          638  CCAAGG               C^C[AT][AT]GG
TaqI          392  TCGA                 T^CGA
Tsp45I        238  GTCAC                ^GT[GC]AC
TthHB8I       392  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          405  GTAC                 GT^AC
              495  GTAC                
AvaII         153  GGTCC                G^G[AT]CC
              327  GGACC               
BbeI          284  GGCGCC               GGCGC^C
              465  GGCGCC              
BcnI          156  CCGGG                CC^[GC]GG
              510  CCGGG               
BsiEI         127  CGGCCG               CG[GA][CT]^CG
              204  CGGCCG              
BsmAI         115  GAGAC                GTCTC(1/5)
              545  GAGAC               
BsrI          281  ACTGG                ACTGG(1/-1)
              413  ACTGG               
DpnII          30  GATC                 ^GATC
              390  GATC                
DsaI           22  CCGCGG               C^C[GA][CT]GG
              330  CCACGG              
EaeI          127  CGGCCG               [CT]^GGCC[GA]
              204  CGGCCG              
EagI          127  CGGCCG               C^GGCCG
              204  CGGCCG              
EclXI         127  CGGCCG               C^GGCCG
              204  CGGCCG              
Eco52I        127  CGGCCG               C^GGCCG
              204  CGGCCG              
HaeII         284  GGCGCC               [GA]GCGC^[CT]
              465  GGCGCC              
KasI          284  GGCGCC               G^GCGCC
              465  GGCGCC              
MaeII         170  ACGT                 A^CGT
              398  ACGT                
MboI           30  GATC                 ^GATC
              390  GATC                
MspA1I         22  CCGCGG               C[AC]G^C[GT]G
              196  CAGCTG              
NaeI          143  GCCGGC               GCC^GGC
              462  GCCGGC              
NarI          284  GGCGCC               GG^CGCC
              465  GGCGCC              
NciI          156  CCGGG                CC^[GC]GG
              510  CCGGG               
NdeII          30  GATC                 ^GATC
              390  GATC                
NgoMIV        143  GCCGGC               G^CCGGC
              462  GCCGGC              
RsaI          405  GTAC                 GT^AC
              495  GTAC                
Sau3AI         30  GATC                 ^GATC
              390  GATC                
SfoI          284  GGCGCC               GGC^GCC
              465  GGCGCC              
StuI          338  AGGCCT               AGG^CCT
              443  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        BanI        
BbsI        BbvI        BmyI        BpuAI       BsaHI       
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BsrFI       BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       ItaI        MboII       
MnlI        MspI        MvaI        MvnI        MwoI        
NlaIII      NlaIV       Sau96I      ScrFI       TseI        
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