Restriction map for pF1KB6228
 (Linear) (Six-base) MAPSORT of: KIBB6228.seq  Check: 7148  from: 1  to: 2220



KIBB6228.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AatII G_ACGT'C

Cuts at:      0     984    2220 
   Size:        984    1236
 
AccI GT'mk_AC

Cuts at:      0     374    2220 
   Size:        374    1846
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1403    1535    2220 
   Size:       1403     132     685

  Fragments arranged by size: 

                1403     685     132
 
AleI CACnn'nnGTG

Cuts at:      0    2053    2220 
   Size:       2053     167
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      40     296    2220 
   Size:         40     256    1924

  Fragments arranged by size: 

                1924     256      40
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     531     563    2220 
   Size:        531      32    1657

  Fragments arranged by size: 

                1657     531      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     582     838    1063    1381    2038    2215    2220 
   Size:        582     256     225     318     657     177       5

  Fragments arranged by size: 

                 657     582     318     256     225     177       5
 
ApaI G_GGCC'C

Cuts at:      0      71     348    1255    2220 
   Size:         71     277     907     965

  Fragments arranged by size: 

                 965     907     277      71
 
AvaI C'yCGr_G

Cuts at:      0     341    2220 
   Size:        341    1879
 
BanI G'GyrC_C

Cuts at:      0     781    1076    1198    1277    2220 
   Size:        781     295     122      79     943

  Fragments arranged by size: 

                 943     781     295     122      79
 
BanII G_rGCy'C

Cuts at:      0      71     348     389     464    1188    1255    1486    1787 
   Size:         71     277      41      75     724      67     231     301

Cuts at:   1787    2220 
   Size:        433

  Fragments arranged by size: 

                 724     433     301     277     231      75      71      67
                  41
 
BbeI G_GCGC'C

Cuts at:      0    1080    2220 
   Size:       1080    1140
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1319    2220 
   Size:       1319     901
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1722    2220 
   Size:       1722     498
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2145    2220 
   Size:       2145      75
 
BglII A'GATC_T

Cuts at:      0    1502    1546    2220 
   Size:       1502      44     674

  Fragments arranged by size: 

                1502     674      44
 
BlpI GC'TnA_GC

Cuts at:      0      59    2220 
   Size:         59    2161
 
Bme1580I G_kGCm'C

Cuts at:      0      71     285     348    1255    1282    2220 
   Size:         71     214      63     907      27     938

  Fragments arranged by size: 

                 938     907     214      71      63      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1872    2026    2220 
   Size:       1872     154     194

  Fragments arranged by size: 

                1872     194     154
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1143    1202    1207    2220 
   Size:       1143      59       5    1013

  Fragments arranged by size: 

                1143    1013      59       5
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     910    1275    1605    1690    2090    2220 
   Size:        202     708     365     330      85     400     130

  Fragments arranged by size: 

                 708     400     365     330     202     130      85
 
Bpu10I CC'TnA_GC

Cuts at:      0     938    2220 
   Size:        938    1282
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385    2220 
   Size:        385    1835
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1827    2220 
   Size:       1827     393
 
BsaHI Gr'CG_yC

Cuts at:      0     981    1077    1847    2220 
   Size:        981      96     770     373

  Fragments arranged by size: 

                 981     770     373      96
 
BsaWI w'CCGG_w

Cuts at:      0    1091    2220 
   Size:       1091    1129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     640     664     670     694     908     938    2220 
   Size:        640      24       6      24     214      30    1282

  Fragments arranged by size: 

                1282     640     214      30      24      24       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     111    1174    1336    1354    1357    1360    1363    1378 
   Size:        111    1063     162      18       3       3       3      15

Cuts at:   1378    1381    1402    1405    1450    1672    1795    1844    2132 
   Size:          3      21       3      45     222     123      49     288

Cuts at:   2132    2220 
   Size:         88

  Fragments arranged by size: 

                1063     288     222     162     123     111      88      49
                  45      21      18      15       3       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0    1999    2065    2220 
   Size:       1999      66     155

  Fragments arranged by size: 

                1999     155      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     375    1034    2220 
   Size:        375     659    1186

  Fragments arranged by size: 

                1186     659     375
 
BsiEI CG_ry'CG

Cuts at:      0     750    2220 
   Size:        750    1470
 
BsiHKAI G_wGCw'C

Cuts at:      0     389    1188    2207    2220 
   Size:        389     799    1019      13

  Fragments arranged by size: 

                1019     799     389      13
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1741    1854    2220 
   Size:       1741     113     366

  Fragments arranged by size: 

                1741     366     113
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     285     348     389     464    1188    1255    1282 
   Size:         71     214      63      41      75     724      67      27

Cuts at:   1282    1486    1787    2207    2220 
   Size:        204     301     420      13

  Fragments arranged by size: 

                 724     420     301     214     204      75      71      67
                  63      41      27      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     674    1453    2220 
   Size:        674     779     767

  Fragments arranged by size: 

                 779     767     674
 
BsrBI CCG'CTC

Cuts at:      0     816    2220 
   Size:        816    1404
 
BsrDI GCAATG_nn'

Cuts at:      0    1145    1647    2220 
   Size:       1145     502     573

  Fragments arranged by size: 

                1145     573     502
 
BsrFI r'CCGG_y

Cuts at:      0     269     353     745    2220 
   Size:        269      84     392    1475

  Fragments arranged by size: 

                1475     392     269      84
 
BssSI C'ACGA_G

Cuts at:      0    2049    2220 
   Size:       2049     171
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     170    2220 
   Size:        170    2050
 
BstBI TT'CG_AA

Cuts at:      0     200    2220 
   Size:        200    2020
 
BstEII G'GTnAC_C

Cuts at:      0    2079    2220 
   Size:       2079     141
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2055    2220 
   Size:       2055     165
 
BstYI r'GATC_y

Cuts at:      0     560     602     630    1059    1395    1502    1546    2220 
   Size:        560      42      28     429     336     107      44     674

  Fragments arranged by size: 

                 674     560     429     336     107      44      42      28
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     245    1655    2220 
   Size:        245    1410     565

  Fragments arranged by size: 

                1410     565     245
 
BtgI C'CryG_G

Cuts at:      0     543     777    2070    2220 
   Size:        543     234    1293     150

  Fragments arranged by size: 

                1293     543     234     150
 
BtsI GCAGTG_nn'

Cuts at:      0     518    2220 
   Size:        518    1702
 
EaeI y'GGCC_r

Cuts at:      0       2     355     747    1100    2171    2220 
   Size:          2     353     392     353    1071      49

  Fragments arranged by size: 

                1071     392     353     353      49       2
 
EagI C'GGCC_G

Cuts at:      0     747    2220 
   Size:        747    1473
 
EarI CTCTTCn'nnn_

Cuts at:      0     197     741    1176    1209    1331    1355    1379    1427 
   Size:        197     544     435      33     122      24      24      48

Cuts at:   1427    1439    1767    1830    2186    2220 
   Size:         12     328      63     356      34

  Fragments arranged by size: 

                 544     435     356     328     197     122      63      48
                  34      33      24      24      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     322     688    2220 
   Size:        322     366    1532

  Fragments arranged by size: 

                1532     366     322
 
EcoICRI GAG'CTC

Cuts at:      0     387    1186    2220 
   Size:        387     799    1034

  Fragments arranged by size: 

                1034     799     387
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     910    1275    1403    1535    1605    1690    2090 
   Size:        202     708     365     128     132      70      85     400

Cuts at:   2090    2220 
   Size:        130

  Fragments arranged by size: 

                 708     400     365     202     132     130     128      85
                  70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     670    1764    2049    2220 
   Size:        670    1094     285     171

  Fragments arranged by size: 

                1094     670     285     171
 
EcoO109I rG'GnC_Cy

Cuts at:      0      67      68     333     539     835    1251    1252    2220 
   Size:         67       1     265     206     296     416       1     968

  Fragments arranged by size: 

                 968     416     296     265     206      67       1       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     226     447    2220 
   Size:        226     221    1773

  Fragments arranged by size: 

                1773     226     221
 
HaeII r_GCGC'y

Cuts at:      0     589    1080    2220 
   Size:        589     491    1140

  Fragments arranged by size: 

                1140     589     491
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     640     672     908     940     971    1003    1744    1776 
   Size:        640      32     236      32      31      32     741      32

Cuts at:   1776    2220 
   Size:        444

  Fragments arranged by size: 

                 741     640     444     236      32      32      32      32
                  31
 
HincII GTy'rAC

Cuts at:      0     801    1044    2220 
   Size:        801     243    1176

  Fragments arranged by size: 

                1176     801     243
 
HindIII A'AGCT_T

Cuts at:      0    1715    2220 
   Size:       1715     505
 
HpaI GTT'AAC

Cuts at:      0     801    2220 
   Size:        801    1419
 
KasI G'GCGC_C

Cuts at:      0    1076    2220 
   Size:       1076    1144
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     608    2220 
   Size:        608    1612
 
MscI TGG'CCA

Cuts at:      0       4    1102    2173    2220 
   Size:          4    1098    1071      47

  Fragments arranged by size: 

                1098    1071      47       4
 
MslI CAynn'nnrTG

Cuts at:      0      22    2053    2220 
   Size:         22    2031     167

  Fragments arranged by size: 

                2031     167      22
 
MspA1I CmG'CkG

Cuts at:      0      55     579     889    2220 
   Size:         55     524     310    1331

  Fragments arranged by size: 

                1331     524     310      55
 
NaeI GCC'GGC

Cuts at:      0     355    2220 
   Size:        355    1865
 
NarI GG'CG_CC

Cuts at:      0    1077    2220 
   Size:       1077    1143
 
NcoI C'CATG_G

Cuts at:      0    2070    2220 
   Size:       2070     150
 
NgoMIV G'CCGG_C

Cuts at:      0     353    2220 
   Size:        353    1867
 
PasI CC'CwG_GG

Cuts at:      0    1272    2220 
   Size:       1272     948
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     570     933    2076    2220 
   Size:        570     363    1143     144

  Fragments arranged by size: 

                1143     570     363     144
 
PfoI T'CCnGG_A

Cuts at:      0    1765    2220 
   Size:       1765     455
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213    2220 
   Size:        181      32    2007

  Fragments arranged by size: 

                2007     181      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     333     539    2220 
   Size:        333     206    1681

  Fragments arranged by size: 

                1681     333     206
 
PspOMI G'GGCC_C

Cuts at:      0      67     344    1251    2220 
   Size:         67     277     907     969

  Fragments arranged by size: 

                 969     907     277      67
 
PstI C_TGCA'G

Cuts at:      0     617     671    1218    2220 
   Size:        617      54     547    1002

  Fragments arranged by size: 

                1002     617     547      54
 
PvuII CAG'CTG

Cuts at:      0      55     579     889    2220 
   Size:         55     524     310    1331

  Fragments arranged by size: 

                1331     524     310      55
 
SacI G_AGCT'C

Cuts at:      0     389    1188    2220 
   Size:        389     799    1032

  Fragments arranged by size: 

                1032     799     389
 
SapI GCTCTTCn'nnn_

Cuts at:      0     741    1176    2220 
   Size:        741     435    1044

  Fragments arranged by size: 

                1044     741     435
 
SbfI CC_TGCA'GG

Cuts at:      0     671    2220 
   Size:        671    1549
 
SfcI C'TryA_G

Cuts at:      0     613     667     875    1029    1214    1498    2220 
   Size:        613      54     208     154     185     284     722

  Fragments arranged by size: 

                 722     613     284     208     185     154      54
 
SfoI GGC'GCC

Cuts at:      0    1078    2220 
   Size:       1078    1142
 
SmlI C'TyrA_G

Cuts at:      0     400    2220 
   Size:        400    1820
 
StyI C'CwwG_G

Cuts at:      0     494     535     903    1194    1235    2015    2024    2070 
   Size:        494      41     368     291      41     780       9      46

Cuts at:   2070    2220 
   Size:        150

  Fragments arranged by size: 

                 780     494     368     291     150      46      41      41
                   9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      92    2220 
   Size:         92    2128
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1565    1597    2220 
   Size:       1565      32     623

  Fragments arranged by size: 

                1565     623      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1110    2073    2220 
   Size:       1110     963     147

  Fragments arranged by size: 

                1110     963     147
 
XmnI GAAnn'nnTTC

Cuts at:      0     196    2220 
   Size:        196    2024
 
ZraI GAC'GTC

Cuts at:      0     982    2220 
   Size:        982    1238
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AleI     AlfI     AloI    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BbsI    BciVI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
     SbfI     SfcI     SfoI     SmlI     StyI    TaqII     TstI     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI     BclI    BfrBI    BmgBI     BmtI    BsaAI    BsaBI
    BsiWI     BsmI    BspEI    BspHI    BsrGI   BssHII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI
     FspI    FspAI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI
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