Restriction map for pF1KB6225
 (Linear) (Six-base) MAPSORT of: KIBB6225.seq  Check: 1000  from: 1  to: 2013



KIBB6225.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1521    2013 
   Size:       1521     492
 
AccI GT'mk_AC

Cuts at:      0     698    1070    1220    2013 
   Size:        698     372     150     793

  Fragments arranged by size: 

                 793     698     372     150
 
Acc65I G'GTAC_C

Cuts at:      0     437    2013 
   Size:        437    1576
 
AclI AA'CG_TT

Cuts at:      0    1984    2013 
   Size:       1984      29
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    2013 
   Size:        623    1390
 
AfeI AGC'GCT

Cuts at:      0     349    2013 
   Size:        349    1664
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     563    2013 
   Size:        563    1450
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1535    1567    2013 
   Size:       1535      32     446

  Fragments arranged by size: 

                1535     446      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     250     434     735    1359    2013 
   Size:        250     184     301     624     654

  Fragments arranged by size: 

                 654     624     301     250     184
 
ApaI G_GGCC'C

Cuts at:      0      10     377     927    2013 
   Size:         10     367     550    1086

  Fragments arranged by size: 

                1086     550     367      10
 
ApoI r'AATT_y

Cuts at:      0    1975    2013 
   Size:       1975      38
 
AvaI C'yCGr_G

Cuts at:      0      89    1085    1778    2013 
   Size:         89     996     693     235

  Fragments arranged by size: 

                 996     693     235      89
 
AvrII C'CTAG_G

Cuts at:      0    1050    2013 
   Size:       1050     963
 
BanI G'GyrC_C

Cuts at:      0     102     437     912    1499    1750    2013 
   Size:        102     335     475     587     251     263

  Fragments arranged by size: 

                 587     475     335     263     251     102
 
BanII G_rGCy'C

Cuts at:      0      10      35     377     537     907     927     970    2013 
   Size:         10      25     342     160     370      20      43    1043

  Fragments arranged by size: 

                1043     370     342     160      43      25      20      10
            
 
BbeI G_GCGC'C

Cuts at:      0     916    1503    2013 
   Size:        916     587     510

  Fragments arranged by size: 

                 916     587     510
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      74    1889    2013 
   Size:         74    1815     124

  Fragments arranged by size: 

                1815     124      74
 
BbvCI CC'TCA_GC

Cuts at:      0     957    1638    2013 
   Size:        957     681     375

  Fragments arranged by size: 

                 957     681     375
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1519    1553    2013 
   Size:       1519      34     460

  Fragments arranged by size: 

                1519     460      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     952    1277    2013 
   Size:        952     325     736

  Fragments arranged by size: 

                 952     736     325
 
BlpI GC'TnA_GC

Cuts at:      0      34     844    1614    2013 
   Size:         34     810     770     399

  Fragments arranged by size: 

                 810     770     399      34
 
Bme1580I G_kGCm'C

Cuts at:      0      10     107     377     927    1495    2013 
   Size:         10      97     270     550     568     518

  Fragments arranged by size: 

                 568     550     518     270      97      10
 
BmgBI CAC'GTC

Cuts at:      0    1586    2013 
   Size:       1586     427
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     761    1377    2013 
   Size:        761     616     636

  Fragments arranged by size: 

                 761     636     616
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     816    2013 
   Size:        816    1197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     659     844     954     984    1273    1381    1426 
   Size:         49     610     185     110      30     289     108      45

Cuts at:   1426    1912    2013 
   Size:        486     101

  Fragments arranged by size: 

                 610     486     289     185     110     108     101      49
                  45      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     957    1328    1638    2013 
   Size:        957     371     310     375

  Fragments arranged by size: 

                 957     375     371     310
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1144    2013 
   Size:       1144     869
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     509     874    1082    2013 
   Size:        509     365     208     931

  Fragments arranged by size: 

                 931     509     365     208
 
BsaAI yAC'GTr

Cuts at:      0     317    1527    2013 
   Size:        317    1210     486

  Fragments arranged by size: 

                1210     486     317
 
BsaHI Gr'CG_yC

Cuts at:      0     684     913    1500    2013 
   Size:        684     229     587     513

  Fragments arranged by size: 

                 684     587     513     229
 
BsaWI w'CCGG_w

Cuts at:      0     113     440     464    2013 
   Size:        113     327      24    1549

  Fragments arranged by size: 

                1549     327     113      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1361    1391    1535    1565    1615    1645    1800    1830 
   Size:       1361      30     144      30      50      30     155      30

Cuts at:   1830    2013 
   Size:        183

  Fragments arranged by size: 

                1361     183     155     144      50      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     517    1803    2013 
   Size:        517    1286     210

  Fragments arranged by size: 

                1286     517     210
 
BseYI C'CCAG_C

Cuts at:      0      28     209     290     350     536     590     803    1119 
   Size:         28     181      81      60     186      54     213     316

Cuts at:   1119    1574    1627    1653    1786    2013 
   Size:        455      53      26     133     227

  Fragments arranged by size: 

                 455     316     227     213     186     181     133      81
                  60      54      53      28      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1549    2013 
   Size:       1549     464
 
BsiEI CG_ry'CG

Cuts at:      0     947    1272    2013 
   Size:        947     325     741

  Fragments arranged by size: 

                 947     741     325
 
BsiHKAI G_wGCw'C

Cuts at:      0     680     907     970    1265    1439    2013 
   Size:        680     227      63     295     174     574

  Fragments arranged by size: 

                 680     574     295     227     174      63
 
BsmI GAATG_Cn'

Cuts at:      0     643    2013 
   Size:        643    1370
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1592    2013 
   Size:       1592     421
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      35     107     377     537     680     907     927 
   Size:         10      25      72     270     160     143     227      20

Cuts at:    927     970    1265    1439    1495    2013 
   Size:         43     295     174      56     518

  Fragments arranged by size: 

                 518     295     270     227     174     160     143      72
                  56      43      25      20      10
 
BspEI T'CCGG_A

Cuts at:      0     113    2013 
   Size:        113    1900
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     555     875    1521    2013 
   Size:        555     320     646     492

  Fragments arranged by size: 

                 646     555     492     320
 
BsrBI CCG'CTC

Cuts at:      0     389    1613    2013 
   Size:        389    1224     400

  Fragments arranged by size: 

                1224     400     389
 
BsrDI GCAATG_nn'

Cuts at:      0     183     398    2013 
   Size:        183     215    1615

  Fragments arranged by size: 

                1615     215     183
 
BsrFI r'CCGG_y

Cuts at:      0     909     946    2013 
   Size:        909      37    1067

  Fragments arranged by size: 

                1067     909      37
 
BssSI C'ACGA_G

Cuts at:      0    1538    2013 
   Size:       1538     475
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1671    1747    2013 
   Size:       1671      76     266

  Fragments arranged by size: 

                1671     266      76
 
BstYI r'GATC_y

Cuts at:      0     278    1322    2013 
   Size:        278    1044     691

  Fragments arranged by size: 

                1044     691     278
 
BtgI C'CryG_G

Cuts at:      0     377    1266    2013 
   Size:        377     889     747

  Fragments arranged by size: 

                 889     747     377
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1870    2013 
   Size:       1870     143
 
BtsI GCAGTG_nn'

Cuts at:      0    1936    2013 
   Size:       1936      77
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     717    2013 
   Size:        717    1296
 
EaeI y'GGCC_r

Cuts at:      0      57     241     836     944    1269    2013 
   Size:         57     184     595     108     325     744

  Fragments arranged by size: 

                 744     595     325     184     108      57
 
EagI C'GGCC_G

Cuts at:      0     944    1269    2013 
   Size:        944     325     744

  Fragments arranged by size: 

                 944     744     325
 
EarI CTCTTCn'nnn_

Cuts at:      0     599     710    1127    1135    2013 
   Size:        599     111     417       8     878

  Fragments arranged by size: 

                 878     599     417     111       8
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     990    2013 
   Size:        990    1023
 
EcoICRI GAG'CTC

Cuts at:      0     905     968    2013 
   Size:        905      63    1045

  Fragments arranged by size: 

                1045     905      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     623     659     844     954     984    1273    1381 
   Size:         49     574      36     185     110      30     289     108

Cuts at:   1381    1426    1912    2013 
   Size:         45     486     101

  Fragments arranged by size: 

                 574     486     289     185     110     108     101      49
                  45      36      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     247     864     924    1401    1783    1808    1871 
   Size:          6     241     617      60     477     382      25      63

Cuts at:   1871    1967    2013 
   Size:         96      46

  Fragments arranged by size: 

                 617     477     382     241      96      63      60      46
                  25       6
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1185    2013 
   Size:       1185     828
 
FseI GG_CCGG'CC

Cuts at:      0     950    2013 
   Size:        950    1063
 
FspI TGC'GCA

Cuts at:      0    1648    2013 
   Size:       1648     365
 
HaeII r_GCGC'y

Cuts at:      0     351     851     916    1503    2013 
   Size:        351     500      65     587     510

  Fragments arranged by size: 

                 587     510     500     351      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1424    1456    1728    1760    1800    1832    2013 
   Size:       1424      32     272      32      40      32     181

  Fragments arranged by size: 

                1424     272     181      40      32      32      32
 
HincII GTy'rAC

Cuts at:      0     420    2013 
   Size:        420    1593
 
KasI G'GCGC_C

Cuts at:      0     912    1499    2013 
   Size:        912     587     514

  Fragments arranged by size: 

                 912     587     514
 
KpnI G_GTAC'C

Cuts at:      0     441    2013 
   Size:        441    1572
 
MfeI C'AATT_G

Cuts at:      0     875    2013 
   Size:        875    1138
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     152    1036    2013 
   Size:        152     884     977

  Fragments arranged by size: 

                 977     884     152
 
MscI TGG'CCA

Cuts at:      0      59     243    2013 
   Size:         59     184    1770

  Fragments arranged by size: 

                1770     184      59
 
MslI CAynn'nnrTG

Cuts at:      0     814    2013 
   Size:        814    1199
 
MspA1I CmG'CkG

Cuts at:      0     486     774     936    1603    1657    2013 
   Size:        486     288     162     667      54     356

  Fragments arranged by size: 

                 667     486     356     288     162      54
 
NaeI GCC'GGC

Cuts at:      0     911     948    2013 
   Size:        911      37    1065

  Fragments arranged by size: 

                1065     911      37
 
NarI GG'CG_CC

Cuts at:      0     913    1500    2013 
   Size:        913     587     513

  Fragments arranged by size: 

                 913     587     513
 
NgoMIV G'CCGG_C

Cuts at:      0     909     946    2013 
   Size:        909      37    1067

  Fragments arranged by size: 

                1067     909      37
 
NspI r_CATG'y

Cuts at:      0     396     985    2013 
   Size:        396     589    1028

  Fragments arranged by size: 

                1028     589     396
 
PasI CC'CwG_GG

Cuts at:      0     250    2013 
   Size:        250    1763
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     250     434    1026    1359    1797    2013 
   Size:        250     184     592     333     438     216

  Fragments arranged by size: 

                 592     438     333     250     216     184
 
PfoI T'CCnGG_A

Cuts at:      0     721    1960    2013 
   Size:        721    1239      53

  Fragments arranged by size: 

                1239     721      53
 
PmlI CAC'GTG

Cuts at:      0     317    2013 
   Size:        317    1696
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1535    1567    2013 
   Size:       1535      32     446

  Fragments arranged by size: 

                1535     446      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     864    1401    1783    1808    1967    2013 
   Size:        864     537     382      25     159      46

  Fragments arranged by size: 

                 864     537     382     159      46      25
 
PshAI GACnn'nnGTC

Cuts at:      0     891    2013 
   Size:        891    1122
 
PspOMI G'GGCC_C

Cuts at:      0       6     373     923    2013 
   Size:          6     367     550    1090

  Fragments arranged by size: 

                1090     550     367       6
 
PstI C_TGCA'G

Cuts at:      0     662     740    1205    2013 
   Size:        662      78     465     808

  Fragments arranged by size: 

                 808     662     465      78
 
PvuII CAG'CTG

Cuts at:      0     486    1657    2013 
   Size:        486    1171     356

  Fragments arranged by size: 

                1171     486     356
 
SacI G_AGCT'C

Cuts at:      0     907     970    2013 
   Size:        907      63    1043

  Fragments arranged by size: 

                1043     907      63
 
SexAI A'CCwGG_T

Cuts at:      0    1352    2013 
   Size:       1352     661
 
SfcI C'TryA_G

Cuts at:      0     630     658     736    1201    2013 
   Size:        630      28      78     465     812

  Fragments arranged by size: 

                 812     630     465      78      28
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     952    2013 
   Size:        952    1061
 
SfoI GGC'GCC

Cuts at:      0     914    1501    2013 
   Size:        914     587     512

  Fragments arranged by size: 

                 914     587     512
 
SgrAI Cr'CCGG_yG

Cuts at:      0     909    2013 
   Size:        909    1104
 
SmaI CCC'GGG

Cuts at:      0      91    2013 
   Size:         91    1922
 
SmlI C'TyrA_G

Cuts at:      0    1159    1778    2013 
   Size:       1159     619     235

  Fragments arranged by size: 

                1159     619     235
 
SphI G_CATG'C

Cuts at:      0     396    2013 
   Size:        396    1617
 
StyI C'CwwG_G

Cuts at:      0     323    1050    1187    1503    2013 
   Size:        323     727     137     316     510

  Fragments arranged by size: 

                 727     510     323     316     137
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     313     525    1732    1942    2013 
   Size:        313     212    1207     210      71

  Fragments arranged by size: 

                1207     313     212     210      71
 
TatI w'GTAC_w

Cuts at:      0    1385    1865    2013 
   Size:       1385     480     148

  Fragments arranged by size: 

                1385     480     148
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      52      84     211     243    1361    1393    1615    1647 
   Size:         52      32     127      32    1118      32     222      32

Cuts at:   1647    1743    1775    2013 
   Size:         96      32     238

  Fragments arranged by size: 

                1118     238     222     127      96      52      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     703    1066    1216    2013 
   Size:        703     363     150     797

  Fragments arranged by size: 

                 797     703     363     150
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     771    1794    2013 
   Size:        771    1023     219

  Fragments arranged by size: 

                1023     771     219
 
XhoI C'TCGA_G

Cuts at:      0    1778    2013 
   Size:       1778     235
 
XmaI C'CCGG_G

Cuts at:      0      89    2013 
   Size:         89    1924
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI     AlfI     AloI
    AlwNI     ApaI     ApoI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI
    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FseI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
    SexAI     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII    AflII   AflIII     AgeI     AhdI     AleI    ApaLI     AscI
     AseI    AsiSI     BaeI    BamHI    BciVI     BclI    BfrBI    BglII
     BmtI    BsaBI    BsiWI    BspHI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII    EcoNI
    EcoRI    EcoRV    FspAI  HindIII     HpaI     MluI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SgfI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     XbaI     XmnI     ZraI
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