Restriction map for pF1KB6215
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AhdI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
BciVI       BclI        BfrI        BlpI        Bpu1102I    
BsaBI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        Eam1105I    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        HindIII     HpaI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MunI        NcoI        NheI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI         1606  AAATTC               [GA]^AATT[CT]
AflIII       1965  ACGTGT               A^C[GA][CT]GT
ApaI         1050  GGGCCC               GGGCC^C
ApoI         1606  AAATTC               [GA]^AATT[CT]
AviII         491  TGCGCA               TGC^GCA
AvrII         684  CCTAGG               C^CTAGG
BalI         1130  TGGCCA               TGG^CCA
BbeI          831  GGCGCC               GGCGC^C
BbvCI         190  CCTCAGC              CCTCAGC(-5/-2)
BcgI           44  GCAGCCTCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          685  CTAG                 C^TAG
BglII         230  AGATCT               A^GATCT
BlnI          684  CCTAGG               C^CTAGG
BsrDI        1559  GCAATG               GCAATG(2/0)
BssHII         17  GCGCGC               G^CGCGC
BstEII       1303  GGTGACC              G^GT[ACGT]ACC
BstPI        1303  GGTGACC              G^GT[ACGT]ACC
BstXI         521  CCACGCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       1478  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          142  GACGCGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1303  GGTGACC              G^GT[ACGT]ACC
FseI          973  GGCCGGCC             GGCCGG^CC
FspI          491  TGCGCA               TGC^GCA
HinfI        1181  GAGTC                G^A[ACGT]TC
KasI          831  GGCGCC               G^GCGCC
MaeI          685  CTAG                 C^TAG
MluNI        1130  TGGCCA               TGG^CCA
MscI         1130  TGGCCA               TGG^CCA
NaeI          974  GCCGGC               GCC^GGC
NarI          831  GGCGCC               GG^CGCC
NdeI          205  CATATG               CA^TATG
NgoMIV        974  GCCGGC               G^CCGGC
NotI           75  GCGGCCGC             GC^GGCCGC
PleI         1181  GAGTC                GAGTC(4/5)
PstI         1993  CTGCAG               CTGCA^G
SfcI         1993  CTGCAG               C^T[GA][CT]AG
SfoI          831  GGCGCC               GGC^GCC
SmaI         1753  CCCGGG               CCC^GGG
Tsp509I      1607  AATT                 ^AATT
XmaCI        1753  CCCGGG               C^CCGGG
XmaI         1753  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AgeI          283  ACCGGT               A^CCGGT
              643  ACCGGT              
AvaII         150  GGTCC                G^G[AT]CC
              722  GGACC               
BamHI         272  GGATCC               G^GATCC
             1526  GGATCC              
BbrPI        1684  CACGTG               CAC^GTG
             1964  CACGTG              
BbsI          139  GAAGAC               GAAGAC(2/6)
              532  GTCTTC              
BpuAI         139  GAAGAC               GAAGAC(2/6)
              532  GTCTTC              
BsaAI        1684  CACGTG               [CT]AC^GT[GA]
             1964  CACGTG              
BsaI          301  GAGACC               GGTCTC(1/5)
             1487  GAGACC              
BsaWI         283  ACCGGT               [AT]^CCGG[AT]
              643  ACCGGT              
BsrBI        1033  GAGCGG               CCGCTC(-3/-3)
             1078  GAGCGG              
DdeI          191  CTCAG                C^T[ACGT]AG
             1555  CTGAG               
EarI          757  CTCTTC               CTCTTC(1/4)
              298  GAAGAG              
HincII        856  GTTGAC               GT[CT]^[GA]AC
             1813  GTCAAC              
HindII        856  GTTGAC               GT[CT]^[GA]AC
             1813  GTCAAC              
Ksp632I       757  CTCTTC               CTCTTC(1/4)
              298  GAAGAG              
MslI          282  CACCGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1738  CACGGGGGTG          
PinAI         283  ACCGGT               A^CCGGT
              643  ACCGGT              
PmaCI        1684  CACGTG               CAC^GTG
             1964  CACGTG              
PmlI         1684  CACGTG               CAC^GTG
             1964  CACGTG              
SfiI          968  GGCCAGGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              989  GGCCGCCCCGGCC       
SgrAI         282  CACCGGTG             C[GA]^CCGG[CT]G
              642  CACCGGTG            


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

Acc65I      AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       Aor51HI     Asp718I     
AspHI       AvaI        BanI        BanII       BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DpnII       DraII       
DsaI        EaeI        EagI        EclXI       Eco47III    
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        KpnI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XcmI        XhoII       
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