Restriction map for pF1KB6894
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        Tru9I       Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AlwI          372  GATCC                GGATC(4/5)
AvaII         363  GGACC                G^G[AT]CC
BanI          337  GGCACC               G^G[CT][GA]CC
BanII          66  GAGCCC               G[GA]GC[CT]^C
BbrPI         478  CACGTG               CAC^GTG
BbsI          529  GTCTTC               GAAGAC(2/6)
BbvCI         390  CCTCAGC              CCTCAGC(-5/-2)
BbvI          467  GCAGC                GCAGC(8/12)
BglI          586  GCCGTGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         529  GTCTTC               GAAGAC(2/6)
BsaAI         478  CACGTG               [CT]AC^GT[GA]
BsiYI          30  CCTGCTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           30  CCTGCTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         362  GGGAC                GGGAC(10/14)
BspHI         131  TCATGA               T^CATGA
BstYI         371  AGATCC               [GA]^GATC[CT]
Cac8I         404  GCAAGC               GC[ACGT]^[ACGT]GC
EcoT14I       333  CCTTGG               C^C[AT][AT]GG
HapII         360  CCGG                 C^CGG
HpaII         360  CCGG                 C^CGG
MflI          371  AGATCC               [GA]^GATC[CT]
MspI          360  CCGG                 C^CGG
PmaCI         478  CACGTG               CAC^GTG
PmlI          478  CACGTG               CAC^GTG
RcaI          131  TCATGA               T^CATGA
SmaI          359  CCCGGG               CCC^GGG
StyI          333  CCTTGG               C^C[AT][AT]GG
TseI          467  GCAGC                G^C[AT]GC
XhoII         371  AGATCC               [GA]^GATC[CT]
XmaCI         359  CCCGGG               C^CCGGG
XmaI          359  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaI          359  CCCGGG               C^[CT]CG[GA]G
              516  CTCGGG              
BcnI          359  CCCGG                CC^[GC]GG
              360  CCGGG               
BsgI           43  CTGCAC               GTGCAG(16/14)
              465  GTGCAG              
BsoBI         359  CCCGGG               C^[CT]CG[GA]G
              516  CTCGGG              
BstF5I        426  CATCC                GGATG(2/0)
              525  GGATG               
BstXI         224  CCAACAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              299  CCAAGCTGGTGG        
DdeI          391  CTCAG                C^T[ACGT]AG
              442  CTTAG               
FauI          267  CCCGC                CCCGC(4/6)
               61  GCGGG               
FokI          426  CATCC                GGATG(9/13)
              525  GGATG               
HincII         88  GTCAAC               GT[CT]^[GA]AC
              554  GTCAAC              
HindII         88  GTCAAC               GT[CT]^[GA]AC
              554  GTCAAC              
HphI           80  TCACC                GGTGA(8/7)
              356  TCACC               
MslI          321  CATAGTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              582  CATCGCCGTG          
NciI          359  CCCGG                CC^[GC]GG
              360  CCGGG               
StuI          435  AGGCCT               AGG^CCT
              599  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AfaI        AluI        BmyI        BsaJI       
Bsp1286I    BsrI        BssKI       BstNI       Cfr13I      
DpnII       EcoRII      Fnu4HI      HaeIII      ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       Tsp45I      
TspRI       
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