Restriction map for pF1KB6834
 (Linear) (Six-base) MAPSORT of: KIBB6834.seq  Check: 3359  from: 1  to: 567



KIBB6834.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     117     567 
   Size:        117     450
 
AflIII A'CryG_T

Cuts at:      0     470     567 
   Size:        470      97
 
AleI CACnn'nnGTG

Cuts at:      0     475     567 
   Size:        475      92
 
BanI G'GyrC_C

Cuts at:      0      28     567 
   Size:         28     539
 
BbeI G_GCGC'C

Cuts at:      0      32     567 
   Size:         32     535
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     162     567 
   Size:        162     405
 
Bme1580I G_kGCm'C

Cuts at:      0     329     567 
   Size:        329     238
 
BmgBI CAC'GTC

Cuts at:      0     149     324     567 
   Size:        149     175     243

  Fragments arranged by size: 

                 243     175     149
 
Bpu10I CC'TnA_GC

Cuts at:      0     389     567 
   Size:        389     178
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     421     567 
   Size:        421     146
 
BsaHI Gr'CG_yC

Cuts at:      0      29     567 
   Size:         29     538
 
BsaWI w'CCGG_w

Cuts at:      0     119     567 
   Size:        119     448
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199     567 
   Size:        199     368
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     567 
   Size:        347     220
 
BsiHKAI G_wGCw'C

Cuts at:      0     563     567 
   Size:        563       4
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     139     567 
   Size:        139     428
 
Bsp1286I G_dGCh'C

Cuts at:      0     329     563     567 
   Size:        329     234       4

  Fragments arranged by size: 

                 329     234       4
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     117     567 
   Size:        117     450
 
BsrFI r'CCGG_y

Cuts at:      0      31     175     567 
   Size:         31     144     392

  Fragments arranged by size: 

                 392     144      31
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     501     548     567 
   Size:        501      47      19

  Fragments arranged by size: 

                 501      47      19
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     567 
   Size:        336     231
 
BstYI r'GATC_y

Cuts at:      0     485     567 
   Size:        485      82
 
BtgI C'CryG_G

Cuts at:      0     329     567 
   Size:        329     238
 
DraIII CAC_nnn'GTG

Cuts at:      0      84     567 
   Size:         84     483
 
EaeI y'GGCC_r

Cuts at:      0     177     434     567 
   Size:        177     257     133

  Fragments arranged by size: 

                 257     177     133
 
EcoO109I rG'GnC_Cy

Cuts at:      0     393     567 
   Size:        393     174
 
FspI TGC'GCA

Cuts at:      0     217     567 
   Size:        217     350
 
FspAI rTGC'GCAy

Cuts at:      0     217     567 
   Size:        217     350
 
HaeII r_GCGC'y

Cuts at:      0      32     197     567 
   Size:         32     165     370

  Fragments arranged by size: 

                 370     165      32
 
KasI G'GCGC_C

Cuts at:      0      28     567 
   Size:         28     539
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     567 
   Size:        137     430
 
MslI CAynn'nnrTG

Cuts at:      0     475     567 
   Size:        475      92
 
MspA1I CmG'CkG

Cuts at:      0      66     567 
   Size:         66     501
 
NaeI GCC'GGC

Cuts at:      0      33     177     567 
   Size:         33     144     390

  Fragments arranged by size: 

                 390     144      33
 
NarI GG'CG_CC

Cuts at:      0      29     567 
   Size:         29     538
 
NcoI C'CATG_G

Cuts at:      0     329     567 
   Size:        329     238
 
NgoMIV G'CCGG_C

Cuts at:      0      31     175     567 
   Size:         31     144     392

  Fragments arranged by size: 

                 392     144      31
 
NspI r_CATG'y

Cuts at:      0     216     567 
   Size:        216     351
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      84     567 
   Size:         84     483
 
PfoI T'CCnGG_A

Cuts at:      0     164     567 
   Size:        164     403
 
PpuMI rG'GwC_Cy

Cuts at:      0     393     567 
   Size:        393     174
 
PvuII CAG'CTG

Cuts at:      0      66     567 
   Size:         66     501
 
SfcI C'TryA_G

Cuts at:      0     104     567 
   Size:        104     463
 
SfoI GGC'GCC

Cuts at:      0      30     567 
   Size:         30     537
 
SgrAI Cr'CCGG_yG

Cuts at:      0      31     567 
   Size:         31     536
 
StyI C'CwwG_G

Cuts at:      0     329     567 
   Size:        329     238
 
TatI w'GTAC_w

Cuts at:      0     188     209     284     567 
   Size:        188      21      75     283

  Fragments arranged by size: 

                 283     188      75      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     336     368     567 
   Size:        336      32     199

  Fragments arranged by size: 

                 336     199      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     336     567 
   Size:        336     231
 

 Enzymes that do cut:

     AarI   AflIII     AleI     BanI     BbeI    BciVI Bme1580I    BmgBI
   Bpu10I     BsaI    BsaHI    BsaWI    BseRI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrFI   BstAPI    BstXI    BstYI     BtgI   DraIII
     EaeI EcoO109I     FspI    FspAI    HaeII     KasI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI    PflMI     PfoI
    PpuMI    PvuII     SfcI     SfoI    SgrAI     StyI     TatI     TstI
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaXI    BseYI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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