Restriction map for pF1KB6829
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI          201  GAATTC               [GA]^AATT[CT]
AlwI           98  GGATC                GGATC(4/5)
ApoI          201  GAATTC               [GA]^AATT[CT]
AspHI         223  GTGCTC               G[AT]GC[AT]^C
AvaII         347  GGTCC                G^G[AT]CC
BanII         380  GAGCCC               G[GA]GC[CT]^C
BbvI          104  GCTGC                GCAGC(8/12)
BcnI           95  CCCGG                CC^[GC]GG
BglII         180  AGATCT               A^GATCT
BpmI          336  CTGGAG               CTGGAG(16/14)
BsaBI         175  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       223  GTGCTC               G[AT]GC[AT]^C
BsmFI         348  GTCCC                GGGAC(10/14)
BsrI          335  ACTGG                ACTGG(1/-1)
BstF5I        450  GGATG                GGATG(2/0)
BstYI         180  AGATCT               [GA]^GATC[CT]
Cac8I         329  GCTGGC               GC[ACGT]^[ACGT]GC
DraII         346  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI          283  GACTTCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      346  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         201  GAATTC               G^AATTC
EcoT14I        60  CCAAGG               C^C[AT][AT]GG
FokI          450  GGATG                GGATG(9/13)
HapII          96  CCGG                 C^CGG
HinfI         434  GATTC                G^A[ACGT]TC
HpaII          96  CCGG                 C^CGG
HphI          227  TCACC                GGTGA(8/7)
MamI          175  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          180  AGATCT               [GA]^GATC[CT]
MspI           96  CCGG                 C^CGG
NciI           95  CCCGG                CC^[GC]GG
PpuMI         346  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         102  CAGCTG               CAG^CTG
SexAI         212  ACCTGGT              A^CC[AT]GGT
SfaNI         175  GATGC                GCATC(5/9)
SmlI          423  CTCAAG               C^T[CT][GA]AG
StyI           60  CCAAGG               C^C[AT][AT]GG
TfiI          434  GATTC                G^A[AT]TC
TseI          104  GCTGC                G^C[AT]GC
Tsp509I       202  AATT                 ^AATT
XhoII         180  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          324  CCGC                 CCGC(-3/-1)
              327  CCGC                
AluI           13  AGCT                 AG^CT
              103  AGCT                
BanI          115  GGCACC               G^G[CT][GA]CC
              241  GGTGCC              
BbsI          154  GAAGAC               GAAGAC(2/6)
              463  GAAGAC              
BglI          107  GCCCAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              244  GCCTTTGTGGC         
BmyI          223  GTGCTC               G[AGT]GC[ACT]^C
              380  GAGCCC              
BpuAI         154  GAAGAC               GAAGAC(2/6)
              463  GAAGAC              
BsaI          163  GAGACC               GGTCTC(1/5)
              354  GGTCTC              
BsiYI          89  CCCAGGCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              231  CCAGGCCGAGG         
BslI           89  CCCAGGCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              231  CCAGGCCGAGG         
Bsp1286I      223  GTGCTC               G[AGT]GC[ACT]^C
              380  GAGCCC              
DdeI            5  CTCAG                C^T[ACGT]AG
               53  CTCAG               
Fnu4HI        104  GCTGC                GC^[ACGT]GC
              326  GCCGC               
ItaI          104  GCTGC                GC^[ACGT]GC
              326  GCCGC               
MboII         154  GAAGA                GAAGA(8/7)
              463  GAAGA               
MspA1I        102  CAGCTG               C[AC]G^C[GT]G
              327  CCGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

BsaJI       BsmAI       BssKI       BstNI       Cfr13I      
DpnII       EcoRII      HaeIII      MboI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       Tru9I       
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com