Restriction map for pF1KB6805
 (Linear) (Six-base) MAPSORT of: KIBB6805.seq  Check: 8496  from: 1  to: 477



KIBB6805.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AleI CACnn'nnGTG

Cuts at:      0     400     477 
   Size:        400      77
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     120     152     477 
   Size:        120      32     325

  Fragments arranged by size: 

                 325     120      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     353     477 
   Size:        353     124
 
Bme1580I G_kGCm'C

Cuts at:      0     114     477 
   Size:        114     363
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     231     477 
   Size:        231     246
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     355     421     477 
   Size:        355      66      56

  Fragments arranged by size: 

                 355      66      56
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     477 
   Size:        114     363
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     373     477 
   Size:        373     104
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     438     477 
   Size:        438      39
 
BstYI r'GATC_y

Cuts at:      0     147     477 
   Size:        147     330
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     211     243     477 
   Size:        211      32     234

  Fragments arranged by size: 

                 234     211      32
 
HincII GTy'rAC

Cuts at:      0      93     477 
   Size:         93     384
 
MslI CAynn'nnrTG

Cuts at:      0     400     477 
   Size:        400      77
 
MspA1I CmG'CkG

Cuts at:      0     400     477 
   Size:        400      77
 
PfoI T'CCnGG_A

Cuts at:      0     124     477 
   Size:        124     353
 
PvuII CAG'CTG

Cuts at:      0     400     477 
   Size:        400      77
 
SfcI C'TryA_G

Cuts at:      0     287     356     477 
   Size:        287      69     121

  Fragments arranged by size: 

                 287     121      69
 

 Enzymes that do cut:

     AleI     AloI     BbsI Bme1580I     BplI     BsaI Bsp1286I    BspMI
    BstXI    BstYI    Hin4I   HincII     MslI   MspA1I     PfoI    PvuII
     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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