Restriction map for
pF1KB6837
(Circular) (Six-base) MAPSORT of: pF1KB6837.seq Check: 3430 from: 1 to: 3629
pF1KB6837 3629 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 667 667
Size: 3629
Acc65I G'GTAC_C
Cuts at: 645 645
Size: 3629
AclI AA'CG_TT
Cuts at: 3469 3469
Size: 3629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1454 1886 2633 2901 1454
Size: 432 747 268 2182
Fragments arranged by size:
2182 747 432 268
AflIII A'CryG_T
Cuts at: 981 2085 3062 981
Size: 1104 977 1548
Fragments arranged by size:
1548 1104 977
AgeI A'CCGG_T
Cuts at: 2017 2017
Size: 3629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 617 617
Size: 3629
AlwNI CAG_nnn'CTG
Cuts at: 934 2501 934
Size: 1567 2062
ApaI G_GGCC'C
Cuts at: 144 144
Size: 3629
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 379 633 2982 3238 379
Size: 254 2349 256 770
Fragments arranged by size:
2349 770 256 254
AseI AT'TA_AT
Cuts at: 20 410 20
Size: 390 3239
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 649 649
Size: 3629
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 645 1059 1307 1342 645
Size: 414 248 35 2932
Fragments arranged by size:
2932 414 248 35
BanII G_rGCy'C
Cuts at: 144 643 1673 144
Size: 499 1030 2100
Fragments arranged by size:
2100 1030 499
BbeI G_GCGC'C
Cuts at: 1063 1311 1063
Size: 248 3381
BbsI GAAGACnn'nnnn_
Cuts at: 248 248
Size: 3629
BbvCI CC'TCA_GC
Cuts at: 425 425
Size: 3629
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 1149 1149
Size: 3629
BglII A'GATC_T
Cuts at: 294 1144 3181 294
Size: 850 2037 742
Fragments arranged by size:
2037 850 742
BlpI GC'TnA_GC
Cuts at: 741 741
Size: 3629
Bme1580I G_kGCm'C
Cuts at: 144 1254 1347 2403 144
Size: 1110 93 1056 1370
Fragments arranged by size:
1370 1110 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 841 922 1257 3401 841
Size: 81 335 2144 1069
Fragments arranged by size:
2144 1069 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 191 304 187
Size: 4 113 3512
Fragments arranged by size:
3512 113 4
Bpu10I CC'TnA_GC
Cuts at: 425 425
Size: 3629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1684 2176 2474 805
Size: 879 492 298 1960
Fragments arranged by size:
1960 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3333 3333
Size: 3629
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 1143 3180 6
Size: 1137 2037 455
Fragments arranged by size:
2037 1137 455
BsaHI Gr'CG_yC
Cuts at: 184 1060 1308 2010 3506 184
Size: 876 248 702 1496 307
Fragments arranged by size:
1496 876 702 307 248
BsaWI w'CCGG_w
Cuts at: 132 818 1339 2017 2291 2438 2849 132
Size: 686 521 678 274 147 411 912
Fragments arranged by size:
912 686 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 302 332 302
Size: 30 3599
BseYI C'CCAG_C
Cuts at: 1054 1812 2389 2885 1054
Size: 758 577 496 1798
Fragments arranged by size:
1798 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1171 1217 2425 104
Size: 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 516 643 1421 1611 2403 516
Size: 127 778 190 792 1742
Fragments arranged by size:
1742 792 778 190 127
BsmBI CGTCTCn'nnnn_
Cuts at: 134 134
Size: 3629
Bsp1286I G_dGCh'C
Cuts at: 144 516 643 1254 1347 1421 1611 1673 2403
Size: 372 127 611 93 74 190 62 730
Cuts at: 2403 144
Size: 1370
Fragments arranged by size:
1370 730 611 372 190 127 93 74
62
BspEI T'CCGG_A
Cuts at: 132 2849 132
Size: 2717 912
BspHI T'CATG_A
Cuts at: 483 2805 483
Size: 2322 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 679 893 1195 1576 679
Size: 214 302 381 2732
Fragments arranged by size:
2732 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1921 1975 3459 3624 50
Size: 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 1541
Size: 3629
BsrFI r'CCGG_y
Cuts at: 1627 1808 2017 1627
Size: 181 209 3239
Fragments arranged by size:
3239 209 181
BssSI C'ACGA_G
Cuts at: 1900 2258 1900
Size: 358 3271
BstAPI GCAn_nnn'nTGC
Cuts at: 1006 1006
Size: 3629
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstXI CCAn_nnnn'nTGG
Cuts at: 621 621
Size: 3629
BstYI r'GATC_y
Cuts at: 1 294 654 1121 1144 1479 1725 2737 2823
Size: 293 360 467 23 335 246 1012 86
Cuts at: 2823 2835 3181 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 360 346 335 293 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1579 1760 1923 1579
Size: 181 163 3285
Fragments arranged by size:
3285 181 163
BtsI GCAGTG_nn'
Cuts at: 257 872 257
Size: 615 3014
DraI TTT'AAA
Cuts at: 384 628 384
Size: 244 3385
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 151 1214 1388 1779 1806 3151 151
Size: 1063 174 391 27 1345 629
Fragments arranged by size:
1345 1063 629 391 174 27
EagI C'GGCC_G
Cuts at: 1214 1214
Size: 3629
EarI CTCTTCn'nnn_
Cuts at: 526 539 1652 1862 526
Size: 13 1113 210 2293
Fragments arranged by size:
2293 1113 210 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1837 2147 2293 3439 1837
Size: 310 146 1146 2027
Fragments arranged by size:
2027 1146 310 146
EcoICRI GAG'CTC
Cuts at: 641 641
Size: 3629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 191 304 1454 1886 2633 2901 187
Size: 4 113 1150 432 747 268 915
Fragments arranged by size:
1150 915 747 432 268 113 4
EcoO109I rG'GnC_Cy
Cuts at: 140 141 768 140
Size: 1 627 3001
Fragments arranged by size:
3001 627 1
EcoRI G'AATT_C
Cuts at: 633 633
Size: 3629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 218 292 1648 218
Size: 74 1356 2199
Fragments arranged by size:
2199 1356 74
FspI TGC'GCA
Cuts at: 1410 1410
Size: 3629
HaeII r_GCGC'y
Cuts at: 1063 1311 2333 3312 1063
Size: 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1501 1503 1533 1471
Size: 30 2 30 3567
Fragments arranged by size:
3567 30 30 2
HincII GTy'rAC
Cuts at: 599 668 599
Size: 69 3560
HpaI GTT'AAC
Cuts at: 599 599
Size: 3629
KasI G'GCGC_C
Cuts at: 1059 1307 1059
Size: 248 3381
KpnI G_GTAC'C
Cuts at: 649 649
Size: 3629
MluI A'CGCG_T
Cuts at: 3062 3062
Size: 3629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 383 530 2300 2484 383
Size: 147 1770 184 1528
Fragments arranged by size:
1770 1528 184 147
MscI TGG'CCA
Cuts at: 1390 1390
Size: 3629
MslI CAynn'nnrTG
Cuts at: 440 1618 1745 440
Size: 1178 127 2324
Fragments arranged by size:
2324 1178 127
MspA1I CmG'CkG
Cuts at: 740 1054 1414 2427 2672 740
Size: 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1810 1810
Size: 3629
NarI GG'CG_CC
Cuts at: 1060 1308 1060
Size: 248 3381
NgoMIV G'CCGG_C
Cuts at: 1808 1808
Size: 3629
NspI r_CATG'y
Cuts at: 682 1182 2089 682
Size: 500 907 2222
Fragments arranged by size:
2222 907 500
PasI CC'CwG_GG
Cuts at: 118 118
Size: 3629
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PfoI T'CCnGG_A
Cuts at: 320 320
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 3597
PspOMI G'GGCC_C
Cuts at: 140 140
Size: 3629
PstI C_TGCA'G
Cuts at: 302 527 676 1361 302
Size: 225 149 685 2570
Fragments arranged by size:
2570 685 225 149
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 643 643
Size: 3629
SalI G'TCGA_C
Cuts at: 666 666
Size: 3629
SapI GCTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
SbfI CC_TGCA'GG
Cuts at: 676 676
Size: 3629
ScaI AGT'ACT
Cuts at: 3146 3146
Size: 3629
SfcI C'TryA_G
Cuts at: 33 236 298 523 672 1357 2350 2541 33
Size: 203 62 225 149 685 993 191 1121
Fragments arranged by size:
1121 993 685 225 203 191 149 62
SfoI GGC'GCC
Cuts at: 1061 1309 1061
Size: 248 3381
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 651 651
Size: 3629
SmlI C'TyrA_G
Cuts at: 784 1699 2191 2453 784
Size: 915 492 262 1960
Fragments arranged by size:
1960 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 682 1182 682
Size: 500 3129
StyI C'CwwG_G
Cuts at: 763 1117 763
Size: 354 3275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 1345 1804 2013 1212
Size: 133 459 209 2828
Fragments arranged by size:
2828 459 209 133
TatI w'GTAC_w
Cuts at: 3144 3144
Size: 3629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3597
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 660 660
Size: 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1124 1124
Size: 3629
XmaI C'CCGG_G
Cuts at: 649 649
Size: 3629
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI