Restriction map for pF1KB6292
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       AccIII      AflII       AgeI        AhdI        
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BalI        BbrPI       BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KspI        MaeI        MfeI        
MluNI       MroI        MscI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        PvuII       RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SwaI        Tth111I     Van91I      
XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       2322  GGTACC               G^GTACC
AccI         1228  GTCTAC               GT^[AC][GT]AC
AccII        1026  CGCG                 CG^CG
AclI         1624  AACGTT               AA^CGTT
AcsI         2165  AAATTC               [GA]^AATT[CT]
AlwNI          41  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2249  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1168  AGCGCT               AGC^GCT
ApoI         2165  AAATTC               [GA]^AATT[CT]
Asp700I       508  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2322  GGTACC               G^GTACC
BamHI        2361  GGATCC               G^GATCC
BbvCI        1493  CCTCAGC              CCTCAGC(-5/-2)
BglI         1456  GCCGCCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         855  CACGTA               [CT]AC^GT[GA]
BsaBI         429  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I       64  TGTACA               T^GTACA
BspHI        2288  TCATGA               T^CATGA
BspLU11I     1470  ACATGT               A^CATGT
BsrBI        1942  GAGCGG               CCGCTC(-3/-3)
BsrGI          64  TGTACA               T^GTACA
BstUI        1026  CGCG                 CG^CG
Bsu36I       2249  CCTCAGG              CC^T[ACGT]AGG
Eco47III     1168  AGCGCT               AGC^GCT
Eco81I       2249  CCTCAGG              CC^T[ACGT]AGG
EcoNI         137  CCTTACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII       824  AAGCTT               A^AGCTT
KpnI         2322  GGTACC               GGTAC^C
MaeIII       2099  GTGAC                ^GT[ACGT]AC
MamI          429  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1025  ACGCGT               A^CGCGT
MspA1I       1585  CCGCTG               C[AC]G^C[GT]G
MvnI         1026  CGCG                 CG^CG
NspI         1470  ACATGT               [GA]CATG^[CT]
PaeR7I       2116  CTCGAG               C^TCGAG
Psp1406I     1624  AACGTT               AA^CGTT
PstI         1744  CTGCAG               CTGCA^G
RcaI         2288  TCATGA               T^CATGA
SexAI        1757  ACCTGGT              A^CC[AT]GGT
SspBI          64  TGTACA               T^GTACA
SspI          290  AATATT               AAT^ATT
StuI         1415  AGGCCT               AGG^CCT
Tsp45I       2099  GTGAC                ^GT[GC]AC
XhoI         2116  CTCGAG               C^TCGAG
XmnI          508  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI         1362  GGCGCC               G[GA]^CG[CT]C
             2034  GGCGCC              
Alw44I       1678  GTGCAC               G^TGCAC
             1906  GTGCAC              
ApaI          797  GGGCCC               GGGCC^C
              981  GGGCCC              
ApaLI        1678  GTGCAC               G^TGCAC
             1906  GTGCAC              
BbeI         1362  GGCGCC               GGCGC^C
             2034  GGCGCC              
BbsI          743  GAAGAC               GAAGAC(2/6)
              811  GAAGAC              
BcgI         1292  CGAGCTCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1235  GCAATCTCCTCG        
BglII        2055  AGATCT               A^GATCT
             2277  AGATCT              
BpuAI         743  GAAGAC               GAAGAC(2/6)
              811  GAAGAC              
BsaHI        1362  GGCGCC               G[GA]^CG[CT]C
             2034  GGCGCC              
BsaI          849  GAGACC               GGTCTC(1/5)
             1436  GGTCTC              
BsmAI         849  GAGAC                GTCTC(1/5)
             1437  GTCTC               
BsmI         1618  GAATGC               GAATGC(1/-1)
             1786  GAATGC              
BspMI        1742  ACCTGC               ACCTGC(4/8)
             1838  ACCTGC              
BsrFI        1561  ACCGGC               [GA]^CCGG[CT]
             2111  ACCGGC              
BstAPI       1622  GCAACGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1874  GCAAGGTGTGC         
Cfr10I       1561  ACCGGC               [GA]^CCGG[CT]
             2111  ACCGGC              
EaeI         1644  CGGCCA               [CT]^GGCC[GA]
             2171  TGGCCG              
EcoT14I       142  CCAAGG               C^C[AT][AT]GG
             2048  CCTTGG              
HgaI         1027  GCGTC                GACGC(5/10)
             1926  GCGTC               
Hin1I        1362  GGCGCC               G[GA]^CG[CT]C
             2034  GGCGCC              
KasI         1362  GGCGCC               G^GCGCC
             2034  GGCGCC              
NarI         1362  GGCGCC               GG^CGCC
             2034  GGCGCC              
PpuMI         683  AGGACCT              [GA]G^G[AT]CC[CT]
              725  AGGACCT             
SacI          936  GAGCTC               GAGCT^C
             1293  GAGCTC              
SfoI         1362  GGCGCC               GGC^GCC
             2034  GGCGCC              
SmaI          395  CCCGGG               CCC^GGG
             1937  CCCGGG              
StyI          142  CCAAGG               C^C[AT][AT]GG
             2048  CCTTGG              
XmaCI         395  CCCGGG               C^CCGGG
             1937  CCCGGG              
XmaI          395  CCCGGG               C^CCGGG
             1937  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       
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