Restriction map for pF1KB6292
 (Linear) (Six-base) MAPSORT of: KIBB6292.seq  Check: 464  from: 1  to: 2367



KIBB6292.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1751    1847    2367 
   Size:       1751      96     520

  Fragments arranged by size: 

                1751     520      96
 
AccI GT'mk_AC

Cuts at:      0    1229    2367 
   Size:       1229    1138
 
Acc65I G'GTAC_C

Cuts at:      0    2322    2367 
   Size:       2322      45
 
AclI AA'CG_TT

Cuts at:      0    1625    2367 
   Size:       1625     742
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1609    1681    2367 
   Size:       1609      72     686

  Fragments arranged by size: 

                1609     686      72
 
AfeI AGC'GCT

Cuts at:      0    1170    2367 
   Size:       1170    1197
 
AflIII A'CryG_T

Cuts at:      0     223    1025    1470    2367 
   Size:        223     802     445     897

  Fragments arranged by size: 

                 897     802     445     223
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     382    2367 
   Size:        382    1985
 
AlwNI CAG_nnn'CTG

Cuts at:      0      46    2367 
   Size:         46    2321
 
ApaI G_GGCC'C

Cuts at:      0     801     985    2367 
   Size:        801     184    1382

  Fragments arranged by size: 

                1382     801     184
 
ApaLI G'TGCA_C

Cuts at:      0    1678    1906    2367 
   Size:       1678     228     461

  Fragments arranged by size: 

                1678     461     228
 
ApoI r'AATT_y

Cuts at:      0    2165    2367 
   Size:       2165     202
 
AvaI C'yCGr_G

Cuts at:      0     395     776    1212    1290    1937    2116    2367 
   Size:        395     381     436      78     647     179     251

  Fragments arranged by size: 

                 647     436     395     381     251     179      78
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     104     137    2367 
   Size:        104      33    2230

  Fragments arranged by size: 

                2230     104      33
 
BamHI G'GATC_C

Cuts at:      0    2361    2367 
   Size:       2361       6
 
BanI G'GyrC_C

Cuts at:      0    1362    2034    2322    2367 
   Size:       1362     672     288      45

  Fragments arranged by size: 

                1362     672     288      45
 
BanII G_rGCy'C

Cuts at:      0     757     801     940     985    1054    1297    1317    2367 
   Size:        757      44     139      45      69     243      20    1050

  Fragments arranged by size: 

                1050     757     243     139      69      45      44      20
            
 
BbeI G_GCGC'C

Cuts at:      0    1366    2038    2367 
   Size:       1366     672     329

  Fragments arranged by size: 

                1366     672     329
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     750     818    2367 
   Size:        750      68    1549

  Fragments arranged by size: 

                1549     750      68
 
BbvCI CC'TCA_GC

Cuts at:      0    1494    2367 
   Size:       1494     873
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1224    1258    1281    1315    2367 
   Size:       1224      34      23      34    1052

  Fragments arranged by size: 

                1224    1052      34      34      23
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1462    2367 
   Size:       1462     905
 
BglII A'GATC_T

Cuts at:      0    2055    2277    2367 
   Size:       2055     222      90

  Fragments arranged by size: 

                2055     222      90
 
Bme1580I G_kGCm'C

Cuts at:      0     223     801     985    1225    1682    1885    1910    2367 
   Size:        223     578     184     240     457     203      25     457

  Fragments arranged by size: 

                 578     457     457     240     223     203     184      25
            
 
BmgBI CAC'GTC

Cuts at:      0     748    2124    2367 
   Size:        748    1376     243

  Fragments arranged by size: 

                1376     748     243
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1893    1956    2367 
   Size:       1893      63     411

  Fragments arranged by size: 

                1893     411      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     924    1312    1874    2367 
   Size:        924     388     562     493

  Fragments arranged by size: 

                 924     562     493     388
 
Bpu10I CC'TnA_GC

Cuts at:      0    1165    1494    2367 
   Size:       1165     329     873

  Fragments arranged by size: 

                1165     873     329
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     916     977    1813    1981    2367 
   Size:        916      61     836     168     386

  Fragments arranged by size: 

                 916     836     386     168      61
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     843    1442    2367 
   Size:        843     599     925

  Fragments arranged by size: 

                 925     843     599
 
BsaAI yAC'GTr

Cuts at:      0     857    2367 
   Size:        857    1510
 
BsaBI GATnn'nnATC

Cuts at:      0     433    2367 
   Size:        433    1934
 
BsaHI Gr'CG_yC

Cuts at:      0    1363    2035    2367 
   Size:       1363     672     332

  Fragments arranged by size: 

                1363     672     332
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     991    1021    1942    1972    2367 
   Size:        991      30     921      30     395

  Fragments arranged by size: 

                 991     921     395      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1231    2233    2238    2367 
   Size:       1231    1002       5     129

  Fragments arranged by size: 

                1231    1002     129       5
 
BseYI C'CCAG_C

Cuts at:      0    1513    2302    2367 
   Size:       1513     789      65

  Fragments arranged by size: 

                1513     789      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1498    1903    1915    2277    2367 
   Size:       1498     405      12     362      90

  Fragments arranged by size: 

                1498     405     362      90      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     589     940    1001    1297    1682    1910    1960    2367 
   Size:        589     351      61     296     385     228      50     407

  Fragments arranged by size: 

                 589     407     385     351     296     228      61      50
            
 
BsmI GAATG_Cn'

Cuts at:      0    1624    1792    2367 
   Size:       1624     168     575

  Fragments arranged by size: 

                1624     575     168
 
Bsp1286I G_dGCh'C

Cuts at:      0     223     589     757     801     940     985    1001    1054 
   Size:        223     366     168      44     139      45      16      53

Cuts at:   1054    1225    1297    1317    1682    1885    1910    1960    2367 
   Size:        171      72      20     365     203      25      50     407

  Fragments arranged by size: 

                 407     366     365     223     203     171     168     139
                  72      53      50      45      44      25      20      16
            
 
BspHI T'CATG_A

Cuts at:      0    2288    2367 
   Size:       2288      79
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1751    1847    2367 
   Size:       1751      96     520

  Fragments arranged by size: 

                1751     520      96
 
BsrBI CCG'CTC

Cuts at:      0    1944    2367 
   Size:       1944     423
 
BsrFI r'CCGG_y

Cuts at:      0    1561    2111    2367 
   Size:       1561     550     256

  Fragments arranged by size: 

                1561     550     256
 
BsrGI T'GTAC_A

Cuts at:      0      64    2367 
   Size:         64    2303
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1628    1880    2367 
   Size:       1628     252     487

  Fragments arranged by size: 

                1628     487     252
 
BstYI r'GATC_y

Cuts at:      0     302    2055    2277    2361    2367 
   Size:        302    1753     222      84       6

  Fragments arranged by size: 

                1753     302     222      84       6
 
Bsu36I CC'TnA_GG

Cuts at:      0    2250    2367 
   Size:       2250     117
 
BtgI C'CryG_G

Cuts at:      0    1442    1460    2174    2367 
   Size:       1442      18     714     193

  Fragments arranged by size: 

                1442     714     193      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1516    2367 
   Size:       1516     851
 
EaeI y'GGCC_r

Cuts at:      0    1644    2171    2367 
   Size:       1644     527     196

  Fragments arranged by size: 

                1644     527     196
 
EarI CTCTTCn'nnn_

Cuts at:      0      50     173     344    1213    2367 
   Size:         50     123     171     869    1154

  Fragments arranged by size: 

                1154     869     171     123      50
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     142    2367 
   Size:        142    2225
 
EcoICRI GAG'CTC

Cuts at:      0     938    1295    2367 
   Size:        938     357    1072

  Fragments arranged by size: 

                1072     938     357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     924    1312    1609    1681    1874    2367 
   Size:        924     388     297      72     193     493

  Fragments arranged by size: 

                 924     493     388     297     193      72
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     141    2367 
   Size:        141    2226
 
EcoO109I rG'GnC_Cy

Cuts at:      0     684     726     798     982    2367 
   Size:        684      42      72     184    1385

  Fragments arranged by size: 

                1385     684     184      72      42
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1149    2367 
   Size:       1149    1218
 
HaeII r_GCGC'y

Cuts at:      0    1172    1366    2038    2367 
   Size:       1172     194     672     329

  Fragments arranged by size: 

                1172     672     329     194
 
HindIII A'AGCT_T

Cuts at:      0     824    2367 
   Size:        824    1543
 
KasI G'GCGC_C

Cuts at:      0    1362    2034    2367 
   Size:       1362     672     333

  Fragments arranged by size: 

                1362     672     333
 
KpnI G_GTAC'C

Cuts at:      0    2326    2367 
   Size:       2326      41
 
MluI A'CGCG_T

Cuts at:      0    1025    2367 
   Size:       1025    1342
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    2367 
   Size:        689    1678
 
MslI CAynn'nnrTG

Cuts at:      0    1785    1869    2140    2367 
   Size:       1785      84     271     227

  Fragments arranged by size: 

                1785     271     227      84
 
MspA1I CmG'CkG

Cuts at:      0    1587    2367 
   Size:       1587     780
 
NarI GG'CG_CC

Cuts at:      0    1363    2035    2367 
   Size:       1363     672     332

  Fragments arranged by size: 

                1363     672     332
 
NspI r_CATG'y

Cuts at:      0    1474    2367 
   Size:       1474     893
 
PasI CC'CwG_GG

Cuts at:      0      89     958    1343    2367 
   Size:         89     869     385    1024

  Fragments arranged by size: 

                1024     869     385      89
 
PciI A'CATG_T

Cuts at:      0    1470    2367 
   Size:       1470     897
 
PfoI T'CCnGG_A

Cuts at:      0     528    2367 
   Size:        528    1839
 
PpuMI rG'GwC_Cy

Cuts at:      0     684     726    2367 
   Size:        684      42    1641

  Fragments arranged by size: 

                1641     684      42
 
PspOMI G'GGCC_C

Cuts at:      0     797     981    2367 
   Size:        797     184    1386

  Fragments arranged by size: 

                1386     797     184
 
PspXI vC'TCGA_Gb

Cuts at:      0    2116    2367 
   Size:       2116     251
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1015    1047    2367 
   Size:       1015      32    1320

  Fragments arranged by size: 

                1320    1015      32
 
PstI C_TGCA'G

Cuts at:      0    1748    2367 
   Size:       1748     619
 
SacI G_AGCT'C

Cuts at:      0     940    1297    2367 
   Size:        940     357    1070

  Fragments arranged by size: 

                1070     940     357
 
SexAI A'CCwGG_T

Cuts at:      0    1757    2367 
   Size:       1757     610
 
SfcI C'TryA_G

Cuts at:      0    1230    1419    1744    1989    2150    2367 
   Size:       1230     189     325     245     161     217

  Fragments arranged by size: 

                1230     325     245     217     189     161
 
SfoI GGC'GCC

Cuts at:      0    1364    2036    2367 
   Size:       1364     672     331

  Fragments arranged by size: 

                1364     672     331
 
SmaI CCC'GGG

Cuts at:      0     397    1939    2367 
   Size:        397    1542     428

  Fragments arranged by size: 

                1542     428     397
 
SmlI C'TyrA_G

Cuts at:      0     931     992    1828    1996    2116    2367 
   Size:        931      61     836     168     120     251

  Fragments arranged by size: 

                 931     836     251     168     120      61
 
SspI AAT'ATT

Cuts at:      0     292    2367 
   Size:        292    2075
 
StuI AGG'CCT

Cuts at:      0    1417    2367 
   Size:       1417     950
 
StyI C'CwwG_G

Cuts at:      0     142    2048    2367 
   Size:        142    1906     319

  Fragments arranged by size: 

                1906     319     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1912    2367 
   Size:       1912     455
 
TatI w'GTAC_w

Cuts at:      0      64     632    2367 
   Size:         64     568    1735

  Fragments arranged by size: 

                1735     568      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     991    1023    1942    1974    2367 
   Size:        991      32     919      32     393

  Fragments arranged by size: 

                 991     919     393      32      32
 
XhoI C'TCGA_G

Cuts at:      0    2116    2367 
   Size:       2116     251
 
XmaI C'CCGG_G

Cuts at:      0     395    1937    2367 
   Size:        395    1542     430

  Fragments arranged by size: 

                1542     430     395
 
XmnI GAAnn'nnTTC

Cuts at:      0     512    2367 
   Size:        512    1855
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BglI    BglII Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BstAPI    BstYI   Bsu36I
     BtgI    BtgZI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII  HindIII     KasI     KpnI     MluI     MmeI
     MslI   MspA1I     NarI     NspI     PasI     PciI     PfoI    PpuMI
   PspOMI    PspXI     PsrI     PstI     SacI    SexAI     SfcI     SfoI
     SmaI     SmlI     SspI     StuI     StyI    TaqII     TatI     TstI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AgeI     AhdI     AleI     AloI     AscI     AseI
    AsiSI    AvrII    BciVI     BclI    BfrBI     BlpI     BmtI     BplI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrDI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII     HpaI     MfeI     MscI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XcmI
     ZraI
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