Restriction map for pF1KB6268
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       Asp718I     
AspI        AviII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BfrI        BglII       BsaAI       
BsaBI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BspLU11I    BsrBI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     CpoI        
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     FseI        FspI        HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacII       SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        2023  GACGTC               GACGT^C
AccIII       2027  TCCGGA               T^CCGGA
AhdI          502  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         500  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AscI           42  GGCGCGCC             GG^CGCGCC
AseI         1794  ATTAAT               AT^TAAT
AsnI         1794  ATTAAT               AT^TAAT
AspEI         502  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2236  CCTAGG               C^CTAGG
BbsI          510  GTCTTC               GAAGAC(2/6)
BbvCI        1230  CCTCAGC              CCTCAGC(-5/-2)
BclI         1758  TGATCA               T^GATCA
BglI         2194  GCCCACTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2236  CCTAGG               C^CTAGG
BlpI          244  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      244  GCTGAGC              GC^T[ACGT]AGC
BpuAI         510  GTCTTC               GAAGAC(2/6)
BsaWI        2027  TCCGGA               [AT]^CCGG[AT]
BseAI        2027  TCCGGA               T^CCGGA
Bsp1407I     1750  TGTACA               T^GTACA
BspDI        1687  ATCGAT               AT^CGAT
BspEI        2027  TCCGGA               T^CCGGA
BspHI         444  TCATGA               T^CATGA
BsrGI        1750  TGTACA               T^GTACA
BssHII         43  GCGCGC               G^CGCGC
BstXI         621  CCAGAAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         583  AGATCC               [GA]^GATC[CT]
CelII         244  GCTGAGC              GC^T[ACGT]AGC
ClaI         1687  ATCGAT               AT^CGAT
Eam1105I      502  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1509  GATATC               GAT^ATC
EcoT22I      1662  ATGCAT               ATGCA^T
FauI         1465  CCCGC                CCCGC(4/6)
FbaI         1758  TGATCA               T^GATCA
HgaI         1903  GACGC                GACGC(5/10)
MflI          583  AGATCC               [GA]^GATC[CT]
MroI         2027  TCCGGA               T^CCGGA
NsiI         1662  ATGCAT               ATGCA^T
PflMI          21  CCAAGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         197  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1794  ATTAAT               AT^TAAT
RcaI          444  TCATGA               T^CATGA
SacI          412  GAGCTC               GAGCT^C
SalI          571  GTCGAC               G^TCGAC
SapI          270  GAAGAGC              GCTCTTC(1/4)
SspBI        1750  TGTACA               T^GTACA
SspI           83  AATATT               AAT^ATT
StuI          785  AGGCCT               AGG^CCT
Van91I         21  CCAAGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          194  CCAGGGACCTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         583  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccI          118  GTAGAC               GT^[AC][GT]AC
              571  GTCGAC              
AcsI         1195  GAATTC               [GA]^AATT[CT]
             1590  GAATTC              
AcyI         1903  GACGCC               G[GA]^CG[CT]C
             2023  GACGTC              
AocI          851  CCTCAGG              CC^T[ACGT]AGG
             2155  CCTGAGG             
ApoI         1195  GAATTC               [GA]^AATT[CT]
             1590  GAATTC              
Asp700I      1586  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2298  GAACAGATTC          
AspHI         412  GAGCTC               G[AT]GC[AT]^C
              730  GAGCAC              
BanI         1425  GGTGCC               G^G[CT][GA]CC
             2202  GGCACC              
BcnI           60  CCCGG                CC^[GC]GG
             1247  CCCGG               
BsaHI        1903  GACGCC               G[GA]^CG[CT]C
             2023  GACGTC              
BsiHKAI       412  GAGCTC               G[AT]GC[AT]^C
              730  GAGCAC              
BspMI        1889  ACCTGC               ACCTGC(4/8)
             1602  GCAGGT              
BsrDI         644  GCAATG               GCAATG(2/0)
             1659  GCAATG              
BsrFI        1392  GCCGGC               [GA]^CCGG[CT]
             2163  GCCGGC              
BssSI         172  CACGAG               CACGAG(-5/-1)
             2018  CTCGTG              
Bsu36I        851  CCTCAGG              CC^T[ACGT]AGG
             2155  CCTGAGG             
Cfr10I       1392  GCCGGC               [GA]^CCGG[CT]
             2163  GCCGGC              
DraII         197  GGGACCT              [GA]G^G[ACGT]CC[CT]
              818  AGGCCCT             
DraIII        427  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2015  CACCTCGTG           
Eco81I        851  CCTCAGG              CC^T[ACGT]AGG
             2155  CCTGAGG             
EcoO109I      197  GGGACCT              [GA]G^G[ACGT]CC[CT]
              818  AGGCCCT             
EcoRI        1195  GAATTC               G^AATTC
             1590  GAATTC              
HaeII          15  GGCGCT               [GA]GCGC^[CT]
              287  AGCGCC              
Hin1I        1903  GACGCC               G[GA]^CG[CT]C
             2023  GACGTC              
HincII        571  GTCGAC               GT[CT]^[GA]AC
             1996  GTCAAC              
HindII        571  GTCGAC               GT[CT]^[GA]AC
             1996  GTCAAC              
HindIII       186  AAGCTT               A^AGCTT
             2288  AAGCTT              
MaeII         569  ACGT                 A^CGT
             2024  ACGT                
NaeI         1392  GCCGGC               GCC^GGC
             2163  GCCGGC              
NciI           60  CCCGG                CC^[GC]GG
             1247  CCCGG               
NcoI          699  CCATGG               C^CATGG
             2105  CCATGG              
NgoMIV       1392  GCCGGC               G^CCGGC
             2163  GCCGGC              
NspI          177  GCATGT               [GA]CATG^[CT]
             1953  ACATGC              
PaeR7I        415  CTCGAG               C^TCGAG
             1501  CTCGAG              
PstI           37  CTGCAG               CTGCA^G
              304  CTGCAG              
PvuII        1762  CAGCTG               CAG^CTG
             2063  CAGCTG              
ScaI         1671  AGTACT               AGT^ACT
             1926  AGTACT              
SmlI          415  CTCGAG               C^T[CT][GA]AG
             1501  CTCGAG              
XhoI          415  CTCGAG               C^TCGAG
             1501  CTCGAG              
XmnI         1586  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2298  GAACAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BalI        BanII       BbvI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoNI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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