Restriction map for pF1KB6268
 (Linear) (Six-base) MAPSORT of: KIBB6268.seq  Check: 6705  from: 1  to: 2316



KIBB6268.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1593    2316 
   Size:       1593     723
 
AatII G_ACGT'C

Cuts at:      0    2027    2316 
   Size:       2027     289
 
AccI GT'mk_AC

Cuts at:      0     119     572    2316 
   Size:        119     453    1744

  Fragments arranged by size: 

                1744     453     119
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     944     946    1262    1882    2316 
   Size:        482     462       2     316     620     434

  Fragments arranged by size: 

                 620     482     462     434     316       2
 
AhdI GACnn_n'nnGTC

Cuts at:      0     507    2316 
   Size:        507    1809
 
AleI CACnn'nnGTG

Cuts at:      0     689    2316 
   Size:        689    1627
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2290    2316 
   Size:       2290      26
 
AlwNI CAG_nnn'CTG

Cuts at:      0     505    2316 
   Size:        505    1811
 
ApoI r'AATT_y

Cuts at:      0    1195    1590    2316 
   Size:       1195     395     726

  Fragments arranged by size: 

                1195     726     395
 
AscI GG'CGCG_CC

Cuts at:      0      43    2316 
   Size:         43    2273
 
AseI AT'TA_AT

Cuts at:      0    1795    2316 
   Size:       1795     521
 
AvaI C'yCGr_G

Cuts at:      0     293     415     664    1501    1907    2316 
   Size:        293     122     249     837     406     409

  Fragments arranged by size: 

                 837     409     406     293     249     122
 
AvrII C'CTAG_G

Cuts at:      0    2236    2316 
   Size:       2236      80
 
BanI G'GyrC_C

Cuts at:      0    1425    2202    2316 
   Size:       1425     777     114

  Fragments arranged by size: 

                1425     777     114
 
BanII G_rGCy'C

Cuts at:      0     416     608    1159    2316 
   Size:        416     192     551    1157

  Fragments arranged by size: 

                1157     551     416     192
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     503    2316 
   Size:        503    1813
 
BbvCI CC'TCA_GC

Cuts at:      0    1231    2316 
   Size:       1231    1085
 
BclI T'GATC_A

Cuts at:      0    1758    2316 
   Size:       1758     558
 
BfrBI ATG'CAT

Cuts at:      0    1664    2316 
   Size:       1664     652
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2200    2316 
   Size:       2200     116
 
BlpI GC'TnA_GC

Cuts at:      0     245    2316 
   Size:        245    2071
 
Bme1580I G_kGCm'C

Cuts at:      0    2196    2316 
   Size:       2196     120
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     146     204    1162    1586    2316 
   Size:        146      58     958     424     730

  Fragments arranged by size: 

                 958     730     424     146      58
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     804    2316 
   Size:        804    1512
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610    1097    1418    2316 
   Size:        610     487     321     898

  Fragments arranged by size: 

                 898     610     487     321
 
Bpu10I CC'TnA_GC

Cuts at:      0    1231    2316 
   Size:       1231    1085
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     414     986    1058    2316 
   Size:        414     572      72    1258

  Fragments arranged by size: 

                1258     572     414      72
 
BsaHI Gr'CG_yC

Cuts at:      0    1904    2024    2316 
   Size:       1904     120     292

  Fragments arranged by size: 

                1904     292     120
 
BsaWI w'CCGG_w

Cuts at:      0    2027    2316 
   Size:       2027     289
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2051    2081    2290    2316 
   Size:       2051      30     209      26

  Fragments arranged by size: 

                2051     209      30      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328     331     465     607     847    1189    1724    2046 
   Size:        328       3     134     142     240     342     535     322

Cuts at:   2046    2072    2173    2316 
   Size:         26     101     143

  Fragments arranged by size: 

                 535     342     328     322     240     143     142     134
                 101      26       3
 
BseYI C'CCAG_C

Cuts at:      0    2149    2316 
   Size:       2149     167
 
BsiHKAI G_wGCw'C

Cuts at:      0     416     734    2316 
   Size:        416     318    1582

  Fragments arranged by size: 

                1582     416     318
 
Bsp1286I G_dGCh'C

Cuts at:      0     416     608     734    1159    2196    2316 
   Size:        416     192     126     425    1037     120

  Fragments arranged by size: 

                1037     425     416     192     126     120
 
BspEI T'CCGG_A

Cuts at:      0    2027    2316 
   Size:       2027     289
 
BspHI T'CATG_A

Cuts at:      0     444    2316 
   Size:        444    1872
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1593    1898    2316 
   Size:       1593     305     418

  Fragments arranged by size: 

                1593     418     305
 
BsrDI GCAATG_nn'

Cuts at:      0     651    1666    2316 
   Size:        651    1015     650

  Fragments arranged by size: 

                1015     651     650
 
BsrFI r'CCGG_y

Cuts at:      0    1392    2163    2316 
   Size:       1392     771     153

  Fragments arranged by size: 

                1392     771     153
 
BsrGI T'GTAC_A

Cuts at:      0    1750    2316 
   Size:       1750     566
 
BssHII G'CGCG_C

Cuts at:      0      43    2316 
   Size:         43    2273
 
BssSI C'ACGA_G

Cuts at:      0     172    2018    2316 
   Size:        172    1846     298

  Fragments arranged by size: 

                1846     298     172
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     628    2316 
   Size:        628    1688
 
BstYI r'GATC_y

Cuts at:      0     583    2316 
   Size:        583    1733
 
Bsu36I CC'TnA_GG

Cuts at:      0     852    2156    2316 
   Size:        852    1304     160

  Fragments arranged by size: 

                1304     852     160
 
BtgI C'CryG_G

Cuts at:      0     353     699     761    1283    2105    2316 
   Size:        353     346      62     522     822     211

  Fragments arranged by size: 

                 822     522     353     346     211      62
 
BtsI GCAGTG_nn'

Cuts at:      0    1489    2196    2316 
   Size:       1489     707     120

  Fragments arranged by size: 

                1489     707     120
 
ClaI AT'CG_AT

Cuts at:      0    1688    2316 
   Size:       1688     628
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1272    1307    2316 
   Size:       1272      35    1009

  Fragments arranged by size: 

                1272    1009      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     432    2020    2316 
   Size:        432    1588     296

  Fragments arranged by size: 

                1588     432     296
 
EaeI y'GGCC_r

Cuts at:      0      50     522    2006    2273    2316 
   Size:         50     472    1484     267      43

  Fragments arranged by size: 

                1484     472     267      50      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     265     501    1548    1856    2316 
   Size:        265     236    1047     308     460

  Fragments arranged by size: 

                1047     460     308     265     236
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      22    2115    2316 
   Size:         22    2093     201

  Fragments arranged by size: 

                2093     201      22
 
EcoICRI GAG'CTC

Cuts at:      0     414    2316 
   Size:        414    1902
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     610     944     946    1097    1262    1418    1882 
   Size:        482     128     334       2     151     165     156     464

Cuts at:   1882    2316 
   Size:        434

  Fragments arranged by size: 

                 482     464     434     334     165     156     151     128
                   2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     307     592     632    2316 
   Size:        307     285      40    1684

  Fragments arranged by size: 

                1684     307     285      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0     198     819    2316 
   Size:        198     621    1497

  Fragments arranged by size: 

                1497     621     198
 
EcoRI G'AATT_C

Cuts at:      0    1195    1590    2316 
   Size:       1195     395     726

  Fragments arranged by size: 

                1195     726     395
 
EcoRV GAT'ATC

Cuts at:      0    1511    2316 
   Size:       1511     805
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2007    2316 
   Size:       2007     309
 
HaeII r_GCGC'y

Cuts at:      0      19     291    2316 
   Size:         19     272    2025

  Fragments arranged by size: 

                2025     272      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     493     525     721     753    1132    1164    1495    1527 
   Size:        493      32     196      32     379      32     331      32

Cuts at:   1527    1855    1887    2051    2083    2316 
   Size:        328      32     164      32     233

  Fragments arranged by size: 

                 493     379     331     328     233     196     164      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     573    1998    2316 
   Size:        573    1425     318

  Fragments arranged by size: 

                1425     573     318
 
HindIII A'AGCT_T

Cuts at:      0     186    2288    2316 
   Size:        186    2102      28

  Fragments arranged by size: 

                2102     186      28
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     162    2316 
   Size:        162    2154
 
MscI TGG'CCA

Cuts at:      0     524    2008    2275    2316 
   Size:        524    1484     267      41

  Fragments arranged by size: 

                1484     524     267      41
 
MslI CAynn'nnrTG

Cuts at:      0     176     443     644     689    2316 
   Size:        176     267     201      45    1627

  Fragments arranged by size: 

                1627     267     201     176      45
 
MspA1I CmG'CkG

Cuts at:      0    1764    1917    2065    2316 
   Size:       1764     153     148     251

  Fragments arranged by size: 

                1764     251     153     148
 
NaeI GCC'GGC

Cuts at:      0    1394    2165    2316 
   Size:       1394     771     151

  Fragments arranged by size: 

                1394     771     151
 
NcoI C'CATG_G

Cuts at:      0     699    2105    2316 
   Size:        699    1406     211

  Fragments arranged by size: 

                1406     699     211
 
NgoMIV G'CCGG_C

Cuts at:      0    1392    2163    2316 
   Size:       1392     771     153

  Fragments arranged by size: 

                1392     771     153
 
NsiI A_TGCA'T

Cuts at:      0    1666    2316 
   Size:       1666     650
 
NspI r_CATG'y

Cuts at:      0     181    1957    2316 
   Size:        181    1776     359

  Fragments arranged by size: 

                1776     359     181
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      27    2316 
   Size:         27    2289
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2290    2316 
   Size:       2290      26
 
PpuMI rG'GwC_Cy

Cuts at:      0     198    2316 
   Size:        198    2118
 
PspXI vC'TCGA_Gb

Cuts at:      0    1501    2316 
   Size:       1501     815
 
PstI C_TGCA'G

Cuts at:      0      41     308    2316 
   Size:         41     267    2008

  Fragments arranged by size: 

                2008     267      41
 
PvuII CAG'CTG

Cuts at:      0    1764    2065    2316 
   Size:       1764     301     251

  Fragments arranged by size: 

                1764     301     251
 
SacI G_AGCT'C

Cuts at:      0     416    2316 
   Size:        416    1900
 
SalI G'TCGA_C

Cuts at:      0     571    2316 
   Size:        571    1745
 
SapI GCTCTTCn'nnn_

Cuts at:      0     265    2316 
   Size:        265    2051
 
ScaI AGT'ACT

Cuts at:      0    1673    1928    2316 
   Size:       1673     255     388

  Fragments arranged by size: 

                1673     388     255
 
SfcI C'TryA_G

Cuts at:      0      37     304     369     579    2316 
   Size:         37     267      65     210    1737

  Fragments arranged by size: 

                1737     267     210      65      37
 
SmlI C'TyrA_G

Cuts at:      0     415    1501    2316 
   Size:        415    1086     815

  Fragments arranged by size: 

                1086     815     415
 
SspI AAT'ATT

Cuts at:      0      85    2316 
   Size:         85    2231
 
StuI AGG'CCT

Cuts at:      0     787    2316 
   Size:        787    1529
 
StyI C'CwwG_G

Cuts at:      0     699    1076    1364    2105    2236    2316 
   Size:        699     377     288     741     131      80

  Fragments arranged by size: 

                 741     699     377     288     131      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     679    2316 
   Size:        679    1637
 
TatI w'GTAC_w

Cuts at:      0    1671    1750    1926    2316 
   Size:       1671      79     176     390

  Fragments arranged by size: 

                1671     390     176      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     568     600     724     756    2316 
   Size:        568      32     124      32    1560

  Fragments arranged by size: 

                1560     568     124      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     201    2316 
   Size:        201    2115
 
XhoI C'TCGA_G

Cuts at:      0     415    1501    2316 
   Size:        415    1086     815

  Fragments arranged by size: 

                1086     815     415
 
XmnI GAAnn'nnTTC

Cuts at:      0    1590    2302    2316 
   Size:       1590     712      14

  Fragments arranged by size: 

                1590     712      14
 
ZraI GAC'GTC

Cuts at:      0    2025    2316 
   Size:       2025     291
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AhdI     AleI     AloI    AlwNI
     ApoI     AscI     AseI     AvaI    AvrII     BanI    BanII     BbsI
    BbvCI     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI
    CspCI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI
     NspI    PflMI     PpiI    PpuMI    PspXI     PstI    PvuII     SacI
     SalI     SapI     ScaI     SfcI     SmlI     SspI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AlfI     ApaI
    ApaLI    AsiSI     BaeI    BamHI     BbeI     BcgI    BciVI    BglII
    BmgBI     BmtI    BpuEI    BsaAI    BsaBI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BsrBI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
     DraI     DrdI     EagI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI
   PspOMI     PsrI     PvuI    RsrII    SacII    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SwaI  Tth111I     XbaI     XmaI
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