Restriction map for
pF1KB6772
##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AciI 26 CCGC CCGC(-3/-1)
AfaI 84 GTAC GT^AC
AluI 50 AGCT AG^CT
AlwI 78 GATCC GGATC(4/5)
ApaI 110 GGGCCC GGGCC^C
Asp700I 271 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 230 TGGCCA TGG^CCA
BanII 110 GGGCCC G[GA]GC[CT]^C
BbsI 16 GAAGAC GAAGAC(2/6)
BbvI 219 GCTGC GCAGC(8/12)
BpuAI 16 GAAGAC GAAGAC(2/6)
BsiYI 90 CCAGTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 90 CCAGTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 83 TGTACA T^GTACA
BsrDI 404 GCAATG GCAATG(2/0)
BsrGI 83 TGTACA T^GTACA
BstAPI 204 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 99 GGATG GGATG(2/0)
BstYI 77 AGATCC [GA]^GATC[CT]
DpnII 78 GATC ^GATC
DraIII 344 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 230 TGGCCA [CT]^GGCC[GA]
Fnu4HI 219 GCTGC GC^[ACGT]GC
FokI 99 GGATG GGATG(9/13)
HapII 327 CCGG C^CGG
HpaII 327 CCGG C^CGG
ItaI 219 GCTGC GC^[ACGT]GC
MboI 78 GATC ^GATC
MflI 77 AGATCC [GA]^GATC[CT]
MluNI 230 TGGCCA TGG^CCA
MscI 230 TGGCCA TGG^CCA
MslI 306 CACCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 49 CAGCTG C[AC]G^C[GT]G
MspI 327 CCGG C^CGG
NdeII 78 GATC ^GATC
NspI 330 GCATGT [GA]CATG^[CT]
PpuMI 149 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 220 CTGCAG CTGCA^G
PvuII 49 CAGCTG CAG^CTG
RsaI 84 GTAC GT^AC
Sau3AI 78 GATC ^GATC
SfaNI 378 GATGC GCATC(5/9)
SfcI 220 CTGCAG C^T[GA][CT]AG
SmlI 145 CTCAAG C^T[CT][GA]AG
SspBI 83 TGTACA T^GTACA
StuI 41 AGGCCT AGG^CCT
TaqI 269 TCGA T^CGA
TseI 219 GCTGC G^C[AT]GC
TthHB8I 269 TCGA T^CGA
XhoII 77 AGATCC [GA]^GATC[CT]
XmnI 271 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AspHI 142 GTGCTC G[AT]GC[AT]^C
202 GAGCAC
AvaII 150 GGTCC G^G[AT]CC
243 GGACC
BanI 250 GGCACC G^G[CT][GA]CC
304 GGCACC
BsiHKAI 142 GTGCTC G[AT]GC[AT]^C
202 GAGCAC
BsrI 345 ACTGG ACTGG(1/-1)
90 CCAGT
MaeIII 5 GTGAC ^GT[ACGT]AC
340 GTCAC
MwoI 204 GCACTTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
213 GCCCATGCTGC
Tsp45I 5 GTGAC ^GT[GC]AC
340 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
BmyI BsaJI Bsp1286I BssKI BstNI
Cfr13I DraII EcoO109I EcoRII HaeIII
HphI MboII MnlI MvaI NlaIII
NlaIV Sau96I ScrFI TspRI