Restriction map for
pF1KB6772
(Circular) (Six-base) MAPSORT of: pF1KB6772.seq Check: 8691 from: 1 to: 3566
pF1KB6772 3566 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 604 604
Size: 3566
Acc65I G'GTAC_C
Cuts at: 582 582
Size: 3566
AclI AA'CG_TT
Cuts at: 3406 3406
Size: 3566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1391 1823 2570 2838 1391
Size: 432 747 268 2119
Fragments arranged by size:
2119 747 432 268
AflIII A'CryG_T
Cuts at: 918 2022 2999 918
Size: 1104 977 1485
Fragments arranged by size:
1485 1104 977
AgeI A'CCGG_T
Cuts at: 1954 1954
Size: 3566
AlwNI CAG_nnn'CTG
Cuts at: 871 2438 871
Size: 1567 1999
ApaI G_GGCC'C
Cuts at: 222 222
Size: 3566
ApaLI G'TGCA_C
Cuts at: 2336 2336
Size: 3566
ApoI r'AATT_y
Cuts at: 570 2919 3175 570
Size: 2349 256 961
Fragments arranged by size:
2349 961 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
AvaI C'yCGr_G
Cuts at: 586 586
Size: 3566
BamHI G'GATC_C
Cuts at: 591 591
Size: 3566
BanI G'GyrC_C
Cuts at: 358 412 582 996 1244 1279 358
Size: 54 170 414 248 35 2645
Fragments arranged by size:
2645 414 248 170 54 35
BanII G_rGCy'C
Cuts at: 222 580 1610 222
Size: 358 1030 2178
Fragments arranged by size:
2178 1030 358
BbeI G_GCGC'C
Cuts at: 1000 1248 1000
Size: 248 3318
BbsI GAAGACnn'nnnn_
Cuts at: 131 131
Size: 3566
BciVI GTATCCnnnnn_n'
Cuts at: 1464 2225 3566 1464
Size: 761 1341 1464
Fragments arranged by size:
1464 1341 761
BclI T'GATC_A
Cuts at: 1086 1086
Size: 3566
BglII A'GATC_T
Cuts at: 1081 3118 1081
Size: 2037 1529
BlpI GC'TnA_GC
Cuts at: 678 678
Size: 3566
Bme1580I G_kGCm'C
Cuts at: 222 361 1191 1284 2340 222
Size: 139 830 93 1056 1448
Fragments arranged by size:
1448 1056 830 139 93
BmrI ACTGGGnnnn_n'
Cuts at: 463 778 859 1194 3338 463
Size: 315 81 335 2144 691
Fragments arranged by size:
2144 691 335 315 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 742 1621 2113 2411 238
Size: 504 879 492 298 1393
Fragments arranged by size:
1393 879 504 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3270 3270
Size: 3566
BsaAI yAC'GTr
Cuts at: 919 919
Size: 3566
BsaBI GATnn'nnATC
Cuts at: 6 1080 3117 6
Size: 1074 2037 455
Fragments arranged by size:
2037 1074 455
BsaHI Gr'CG_yC
Cuts at: 997 1245 1947 3443 997
Size: 248 702 1496 1120
Fragments arranged by size:
1496 1120 702 248
BsaWI w'CCGG_w
Cuts at: 755 1276 1954 2228 2375 2786 755
Size: 521 678 274 147 411 1535
Fragments arranged by size:
1535 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 991 1749 2326 2822 991
Size: 758 577 496 1735
Fragments arranged by size:
1735 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1108 1154 2362 104
Size: 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 46
BsiHKAI G_wGCw'C
Cuts at: 254 314 580 1358 1548 2340 254
Size: 60 266 778 190 792 1480
Fragments arranged by size:
1480 792 778 266 190 60
Bsp1286I G_dGCh'C
Cuts at: 222 254 314 361 580 1191 1284 1358 1548
Size: 32 60 47 219 611 93 74 190
Cuts at: 1548 1610 2340 222
Size: 62 730 1448
Fragments arranged by size:
1448 730 611 219 190 93 74 62
60 47 32
BspEI T'CCGG_A
Cuts at: 2786 2786
Size: 3566
BspHI T'CATG_A
Cuts at: 2742 2742
Size: 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 616 830 1132 1513 616
Size: 214 302 381 2669
Fragments arranged by size:
2669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1858 1912 3396 3561 50
Size: 1808 54 1484 165 55
Fragments arranged by size:
1808 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 519 1478 519
Size: 959 2607
BsrFI r'CCGG_y
Cuts at: 1564 1745 1954 1564
Size: 181 209 3176
Fragments arranged by size:
3176 209 181
BsrGI T'GTAC_A
Cuts at: 191 191
Size: 3566
BssSI C'ACGA_G
Cuts at: 1837 2195 1837
Size: 358 3208
BstAPI GCAn_nnn'nTGC
Cuts at: 318 943 318
Size: 625 2941
BstBI TT'CG_AA
Cuts at: 1927 1927
Size: 3566
BstYI r'GATC_y
Cuts at: 1 185 591 1058 1081 1416 1662 2674 2760
Size: 184 406 467 23 335 246 1012 86
Cuts at: 2760 2772 3118 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 406 346 335 246 184
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1516 1697 1860 1516
Size: 181 163 3222
Fragments arranged by size:
3222 181 163
BtsI GCAGTG_nn'
Cuts at: 809 809
Size: 3566
DraI TTT'AAA
Cuts at: 565 565
Size: 3566
DraIII CAC_nnn'GTG
Cuts at: 457 457
Size: 3566
DrdI GACnn_nn'nnGTC
Cuts at: 1272 2130 1272
Size: 858 2708
EaeI y'GGCC_r
Cuts at: 338 1151 1325 1716 1743 3088 338
Size: 813 174 391 27 1345 816
Fragments arranged by size:
1345 816 813 391 174 27
EagI C'GGCC_G
Cuts at: 1151 1151
Size: 3566
EarI CTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1774 2084 2230 3376 1774
Size: 310 146 1146 1964
Fragments arranged by size:
1964 1146 310 146
EcoICRI GAG'CTC
Cuts at: 578 578
Size: 3566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1391 1823 2570 2838 1391
Size: 432 747 268 2119
Fragments arranged by size:
2119 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 218 219 258 705 218
Size: 1 39 447 3079
Fragments arranged by size:
3079 447 39 1
EcoRI G'AATT_C
Cuts at: 570 570
Size: 3566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 168 1585 168
Size: 1417 2149
FspI TGC'GCA
Cuts at: 1347 1347
Size: 3566
HaeII r_GCGC'y
Cuts at: 1000 1248 2270 3249 1000
Size: 248 1022 979 1317
Fragments arranged by size:
1317 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1408 1438 1440 1470 1408
Size: 30 2 30 3504
Fragments arranged by size:
3504 30 30 2
HincII GTy'rAC
Cuts at: 605 605
Size: 3566
KasI G'GCGC_C
Cuts at: 996 1244 996
Size: 248 3318
KpnI G_GTAC'C
Cuts at: 586 586
Size: 3566
MluI A'CGCG_T
Cuts at: 2999 2999
Size: 3566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2421 2237
Size: 184 3382
MscI TGG'CCA
Cuts at: 340 1327 340
Size: 987 2579
MslI CAynn'nnrTG
Cuts at: 418 1555 1682 418
Size: 1137 127 2302
Fragments arranged by size:
2302 1137 127
MspA1I CmG'CkG
Cuts at: 159 677 991 1351 2364 2609 159
Size: 518 314 360 1013 245 1116
Fragments arranged by size:
1116 1013 518 360 314 245
NaeI GCC'GGC
Cuts at: 1747 1747
Size: 3566
NarI GG'CG_CC
Cuts at: 997 1245 997
Size: 248 3318
NgoMIV G'CCGG_C
Cuts at: 1745 1745
Size: 3566
NspI r_CATG'y
Cuts at: 442 619 1119 2026 442
Size: 177 500 907 1982
Fragments arranged by size:
1982 907 500 177
PasI CC'CwG_GG
Cuts at: 222 222
Size: 3566
PciI A'CATG_T
Cuts at: 2022 2022
Size: 3566
PmeI GTTT'AAAC
Cuts at: 565 565
Size: 3566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 3534
PpuMI rG'GwC_Cy
Cuts at: 258 258
Size: 3566
PspOMI G'GGCC_C
Cuts at: 218 218
Size: 3566
PstI C_TGCA'G
Cuts at: 332 613 1298 332
Size: 281 685 2600
Fragments arranged by size:
2600 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3566
PvuII CAG'CTG
Cuts at: 159 991 1351 159
Size: 832 360 2374
Fragments arranged by size:
2374 832 360
RsrII CG'GwC_CG
Cuts at: 1761 1761
Size: 3566
SacI G_AGCT'C
Cuts at: 580 580
Size: 3566
SalI G'TCGA_C
Cuts at: 603 603
Size: 3566
SapI GCTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
SbfI CC_TGCA'GG
Cuts at: 613 613
Size: 3566
ScaI AGT'ACT
Cuts at: 3083 3083
Size: 3566
SfcI C'TryA_G
Cuts at: 33 328 609 1294 2287 2478 33
Size: 295 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 295 281 191
SfoI GGC'GCC
Cuts at: 998 1246 998
Size: 248 3318
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
SmaI CCC'GGG
Cuts at: 588 588
Size: 3566
SmlI C'TyrA_G
Cuts at: 253 721 1636 2128 2390 253
Size: 468 915 492 262 1429
Fragments arranged by size:
1429 915 492 468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3566
SphI G_CATG'C
Cuts at: 619 1119 619
Size: 500 3066
StuI AGG'CCT
Cuts at: 151 151
Size: 3566
StyI C'CwwG_G
Cuts at: 700 1054 700
Size: 354 3212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 182 412 445 1149 1282 1741 1950 182
Size: 230 33 704 133 459 209 1798
Fragments arranged by size:
1798 704 459 230 209 133 33
TatI w'GTAC_w
Cuts at: 191 3081 191
Size: 2890 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 374 406 3083 3115 374
Size: 32 2677 32 825
Fragments arranged by size:
2677 825 32 32
Tth111I GACn'n_nGTC
Cuts at: 1363 1363
Size: 3566
XbaI T'CTAG_A
Cuts at: 597 597
Size: 3566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1061 1061
Size: 3566
XmaI C'CCGG_G
Cuts at: 586 586
Size: 3566
XmnI GAAnn'nnTTC
Cuts at: 383 383
Size: 3566
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI