(Linear) (Six-base) MAPSORT of: KIBB6772.seq  Check: 5917  from: 1  to: 453



KIBB6772.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

ApaI G_GGCC'C

Cuts at:      0     114     453 
   Size:        114     339
 
BanI G'GyrC_C

Cuts at:      0     250     304     453 
   Size:        250      54     149

  Fragments arranged by size: 

                 250     149      54
 
BanII G_rGCy'C

Cuts at:      0     114     453 
   Size:        114     339
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      23     453 
   Size:         23     430
 
Bme1580I G_kGCm'C

Cuts at:      0     114     253     453 
   Size:        114     139     200

  Fragments arranged by size: 

                 200     139     114
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     355     453 
   Size:        355      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     453 
   Size:        130     323
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     206     453 
   Size:        146      60     247

  Fragments arranged by size: 

                 247     146      60
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     146     206     253     453 
   Size:        114      32      60      47     200

  Fragments arranged by size: 

                 200     114      60      47      32
 
BsrDI GCAATG_nn'

Cuts at:      0     411     453 
   Size:        411      42
 
BsrGI T'GTAC_A

Cuts at:      0      83     453 
   Size:         83     370
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     210     453 
   Size:        210     243
 
BstYI r'GATC_y

Cuts at:      0      77     453 
   Size:         77     376
 
DraIII CAC_nnn'GTG

Cuts at:      0     349     453 
   Size:        349     104
 
EaeI y'GGCC_r

Cuts at:      0     230     453 
   Size:        230     223
 
EcoO109I rG'GnC_Cy

Cuts at:      0     110     111     150     453 
   Size:        110       1      39     303

  Fragments arranged by size: 

                 303     110      39       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      60     453 
   Size:         60     393
 
MscI TGG'CCA

Cuts at:      0     232     453 
   Size:        232     221
 
MslI CAynn'nnrTG

Cuts at:      0     310     453 
   Size:        310     143
 
MspA1I CmG'CkG

Cuts at:      0      51     453 
   Size:         51     402
 
NspI r_CATG'y

Cuts at:      0     334     453 
   Size:        334     119
 
PasI CC'CwG_GG

Cuts at:      0     114     453 
   Size:        114     339
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     453 
   Size:        150     303
 
PspOMI G'GGCC_C

Cuts at:      0     110     453 
   Size:        110     343
 
PstI C_TGCA'G

Cuts at:      0     224     453 
   Size:        224     229
 
PvuII CAG'CTG

Cuts at:      0      51     453 
   Size:         51     402
 
SfcI C'TryA_G

Cuts at:      0     220     453 
   Size:        220     233
 
SmlI C'TyrA_G

Cuts at:      0     145     453 
   Size:        145     308
 
StuI AGG'CCT

Cuts at:      0      43     453 
   Size:         43     410
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      74     304     337     453 
   Size:         74     230      33     116

  Fragments arranged by size: 

                 230     116      74      33
 
TatI w'GTAC_w

Cuts at:      0      83     453 
   Size:         83     370
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     266     298     453 
   Size:        266      32     155

  Fragments arranged by size: 

                 266     155      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     275     453 
   Size:        275     178
 

 Enzymes that do cut:

     ApaI     BanI    BanII     BbsI Bme1580I     BmrI    BpuEI  BsiHKAI
 Bsp1286I    BsrDI    BsrGI   BstAPI    BstYI   DraIII     EaeI EcoO109I
     FalI     MscI     MslI   MspA1I     NspI     PasI    PpuMI   PspOMI
     PstI    PvuII     SfcI     SmlI     StuI    TaqII     TatI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI