Restriction map for pF1KB6752
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvrII       BalI        
BamHI       BanII       BbrPI       BbvCI       BcgI        
BciVI       BfaI        BfrI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        Bsp1286I    BspDI       BspEI       BspHI       
BsrBI       BsrDI       BsrI        BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
HincII      HindII      HindIII     HinfI       HpaI        
KpnI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfcI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          286  GTCTAC               GT^[AC][GT]AC
AfaI          108  GTAC                 GT^AC
AflIII         80  ACATGT               A^C[GA][CT]GT
AlwNI          70  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AviII         179  TGCGCA               TGC^GCA
BanI          198  GGCGCC               G^G[CT][GA]CC
BbeI          198  GGCGCC               GGCGC^C
BbsI          228  GAAGAC               GAAGAC(2/6)
BclI          248  TGATCA               T^GATCA
BglI          386  GCCGGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         228  GAAGAC               GAAGAC(2/6)
BseRI         411  CTCCTC               GAGGAG(10/8)
BsmFI         242  GGGAC                GGGAC(10/14)
Bsp1407I      107  TGTACA               T^GTACA
BspLU11I       80  ACATGT               A^CATGT
BspMI         236  ACCTGC               ACCTGC(4/8)
BsrFI         386  GCCGGC               [GA]^CCGG[CT]
BsrGI         107  TGTACA               T^GTACA
BssHII        115  GCGCGC               G^CGCGC
Cfr10I        386  GCCGGC               [GA]^CCGG[CT]
DraII         140  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoNI         177  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      140  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       206  CCATGG               C^C[AT][AT]GG
FbaI          248  TGATCA               T^GATCA
FseI          385  GGCCGGCC             GGCCGG^CC
FspI          179  TGCGCA               TGC^GCA
KasI          198  GGCGCC               G^GCGCC
KspI           51  CCGCGG               CCGC^GG
NaeI          386  GCCGGC               GCC^GGC
NarI          198  GGCGCC               GG^CGCC
NcoI          206  CCATGG               C^CATGG
NgoMIV        386  GCCGGC               G^CCGGC
NspI           80  ACATGT               [GA]CATG^[CT]
RsaI          108  GTAC                 GT^AC
SacII          51  CCGCGG               CCGC^GG
SexAI         308  ACCAGGT              A^CC[AT]GGT
SfoI          198  GGCGCC               GGC^GCC
SmlI          430  CTCAAG               C^T[CT][GA]AG
SspBI         107  TGTACA               T^GTACA
StyI          206  CCATGG               C^C[AT][AT]GG
TaqI           65  TCGA                 T^CGA
TspRI         111  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        65  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
              116  CGCG                
AcyI          198  GGCGCC               G[GA]^CG[CT]C
              283  GGCGTC              
AvaI          259  CCCGAG               C^[CT]CG[GA]G
              325  CCCGAG              
AvaII          29  GGACC                G^G[AT]CC
               60  GGACC               
BcnI          138  CCGGG                CC^[GC]GG
              391  CCCGG               
BsaHI         198  GGCGCC               G[GA]^CG[CT]C
              283  GGCGTC              
BsoBI         259  CCCGAG               C^[CT]CG[GA]G
              325  CCCGAG              
BstAPI         93  GCAGCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              159  GCACTCCCTGC         
BstF5I        252  CATCC                GGATG(2/0)
              423  CATCC               
BstNI         309  CCAGG                CC^[AT]GG
              345  CCTGG               
BstUI          52  CGCG                 CG^CG
              116  CGCG                
DsaI           51  CCGCGG               C^C[GA][CT]GG
              206  CCATGG              
EaeI          336  CGGCCA               [CT]^GGCC[GA]
              384  TGGCCG              
EcoRII        309  CCAGG                ^CC[AT]GG
              345  CCTGG               
FauI          416  CCCGC                CCCGC(4/6)
              240  GCGGG               
FokI          252  CATCC                GGATG(9/13)
              423  CATCC               
HaeII         173  AGCGCC               [GA]GCGC^[CT]
              198  GGCGCC              
HgaI          231  GACGC                GACGC(5/10)
              284  GCGTC               
Hin1I         198  GGCGCC               G[GA]^CG[CT]C
              283  GGCGTC              
MvaI          309  CCAGG                CC^[AT]GG
              345  CCTGG               
MvnI           52  CGCG                 CG^CG
              116  CGCG                
NciI          138  CCGGG                CC^[GC]GG
              391  CCCGG               
PflMI          69  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              339  CCACTACCTGG         
SfaNI          99  GATGC                GCATC(5/9)
              395  GCATC               
Van91I         69  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              339  CCACTACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BssKI       Cac8I       CfoI        Cfr13I      
DpnII       Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MboI        
MboII       MnlI        MspA1I      MspI        MwoI        
NdeII       NlaIII      NlaIV       PvuII       Sau3AI      
Sau96I      ScrFI       TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com