Restriction map for
pF1KB6752
(Linear) (Six-base) MAPSORT of: KIBB6752.seq Check: 1578 from: 1 to: 435
KIBB6752.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 245 435
Size: 245 190
AccI GT'mk_AC
Cuts at: 0 287 435
Size: 287 148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 435
Size: 190 245
AflIII A'CryG_T
Cuts at: 0 80 435
Size: 80 355
AlwNI CAG_nnn'CTG
Cuts at: 0 75 435
Size: 75 360
AvaI C'yCGr_G
Cuts at: 0 259 325 435
Size: 259 66 110
Fragments arranged by size:
259 110 66
BanI G'GyrC_C
Cuts at: 0 198 435
Size: 198 237
BbeI G_GCGC'C
Cuts at: 0 202 435
Size: 202 233
BbsI GAAGACnn'nnnn_
Cuts at: 0 235 435
Size: 235 200
BclI T'GATC_A
Cuts at: 0 248 435
Size: 248 187
BglI GCCn_nnn'nGGC
Cuts at: 0 392 435
Size: 392 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 435
Size: 415 20
BsaHI Gr'CG_yC
Cuts at: 0 199 284 435
Size: 199 85 151
Fragments arranged by size:
199 151 85
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 402 435
Size: 402 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 435
Size: 245 190
BsrFI r'CCGG_y
Cuts at: 0 386 435
Size: 386 49
BsrGI T'GTAC_A
Cuts at: 0 107 435
Size: 107 328
BssHII G'CGCG_C
Cuts at: 0 115 435
Size: 115 320
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 165 435
Size: 99 66 270
Fragments arranged by size:
270 99 66
BtgI C'CryG_G
Cuts at: 0 51 206 435
Size: 51 155 229
Fragments arranged by size:
229 155 51
EaeI y'GGCC_r
Cuts at: 0 336 384 435
Size: 336 48 51
Fragments arranged by size:
336 51 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 165 435
Size: 165 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 435
Size: 190 245
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 435
Size: 181 254
EcoO109I rG'GnC_Cy
Cuts at: 0 141 435
Size: 141 294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 48 435
Size: 48 387
FseI GG_CCGG'CC
Cuts at: 0 390 435
Size: 390 45
FspI TGC'GCA
Cuts at: 0 181 435
Size: 181 254
HaeII r_GCGC'y
Cuts at: 0 177 202 435
Size: 177 25 233
Fragments arranged by size:
233 177 25
KasI G'GCGC_C
Cuts at: 0 198 435
Size: 198 237
MspA1I CmG'CkG
Cuts at: 0 53 72 96 105 123 435
Size: 53 19 24 9 18 312
Fragments arranged by size:
312 53 24 19 18 9
NaeI GCC'GGC
Cuts at: 0 388 435
Size: 388 47
NarI GG'CG_CC
Cuts at: 0 199 435
Size: 199 236
NcoI C'CATG_G
Cuts at: 0 206 435
Size: 206 229
NgoMIV G'CCGG_C
Cuts at: 0 386 435
Size: 386 49
NspI r_CATG'y
Cuts at: 0 84 435
Size: 84 351
PciI A'CATG_T
Cuts at: 0 80 435
Size: 80 355
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 345 435
Size: 75 270 90
Fragments arranged by size:
270 90 75
PvuII CAG'CTG
Cuts at: 0 72 96 105 435
Size: 72 24 9 330
Fragments arranged by size:
330 72 24 9
SacII CC_GC'GG
Cuts at: 0 54 435
Size: 54 381
SexAI A'CCwGG_T
Cuts at: 0 308 435
Size: 308 127
SfoI GGC'GCC
Cuts at: 0 200 435
Size: 200 235
SmlI C'TyrA_G
Cuts at: 0 430 435
Size: 430 5
StyI C'CwwG_G
Cuts at: 0 206 435
Size: 206 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 435
Size: 421 14
TatI w'GTAC_w
Cuts at: 0 107 435
Size: 107 328
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI AvaI BanI BbeI
BbsI BclI BglI BpuEI BsaHI BseRI BspMI BsrFI
BsrGI BssHII BstAPI BtgI EaeI EciI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII KasI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PvuII SacII
SexAI SfoI SmlI StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbvCI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrDI BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI