Restriction map for pF1KB6250
 (Linear) (Six-base) MAPSORT of: KIBB6250.seq  Check: 5712  from: 1  to: 2046



KIBB6250.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

Acc65I G'GTAC_C

Cuts at:      0     284    2046 
   Size:        284    1762
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     837     921    1108    1257    1341    1425    1509 
   Size:        105     732      84     187     149      84      84      84

Cuts at:   1509    1528    1593    1761    1845    1929    2046 
   Size:         19      65     168      84      84     117

  Fragments arranged by size: 

                 732     187     168     149     117     105      84      84
                  84      84      84      84      65      19
 
AfeI AGC'GCT

Cuts at:      0    1774    2046 
   Size:       1774     272
 
AflIII A'CryG_T

Cuts at:      0    1426    2046 
   Size:       1426     620
 
AleI CACnn'nnGTG

Cuts at:      0     476    2046 
   Size:        476    1570
 
BanI G'GyrC_C

Cuts at:      0     284    1643    2046 
   Size:        284    1359     403

  Fragments arranged by size: 

                1359     403     284
 
BanII G_rGCy'C

Cuts at:      0     529     555    1453    1873    1957    2046 
   Size:        529      26     898     420      84      89

  Fragments arranged by size: 

                 898     529     420      89      84      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     610    1851    2046 
   Size:         39     571    1241     195

  Fragments arranged by size: 

                1241     571     195      39
 
BfrBI ATG'CAT

Cuts at:      0    1006    1258    2046 
   Size:       1006     252     788

  Fragments arranged by size: 

                1006     788     252
 
BglII A'GATC_T

Cuts at:      0     317    1455    2046 
   Size:        317    1138     591

  Fragments arranged by size: 

                1138     591     317
 
BmgBI CAC'GTC

Cuts at:      0      36    2046 
   Size:         36    2010
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     543     749    1169    1253    1421    1521    1589 
   Size:        133     410     206     420      84     168     100      68

Cuts at:   1589    1673    1887    2046 
   Size:         84     214     159

  Fragments arranged by size: 

                 420     410     214     206     168     159     133     100
                  84      84      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1941    2046 
   Size:       1941     105
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     138    1821    2046 
   Size:        138    1683     225

  Fragments arranged by size: 

                1683     225     138
 
BsaWI w'CCGG_w

Cuts at:      0    1903    2046 
   Size:       1903     143
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     672     702    1944    1974    2046 
   Size:        672      30    1242      30      72

  Fragments arranged by size: 

                1242     672      72      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70      73     219     785     850    1102    1186    1354 
   Size:         70       3     146     566      65     252      84     168

Cuts at:   1354    1690    1942    2046 
   Size:        336     252     104

  Fragments arranged by size: 

                 566     336     252     252     168     146     104      84
                  70      65       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1610    2046 
   Size:       1610     436
 
BsiHKAI G_wGCw'C

Cuts at:      0     529    1453    1873    1957    2046 
   Size:        529     924     420      84      89

  Fragments arranged by size: 

                 924     529     420      89      84
 
Bsp1286I G_dGCh'C

Cuts at:      0     529     555    1453    1873    1957    2046 
   Size:        529      26     898     420      84      89

  Fragments arranged by size: 

                 898     529     420      89      84      26
 
BspHI T'CATG_A

Cuts at:      0     294     963    2046 
   Size:        294     669    1083

  Fragments arranged by size: 

                1083     669     294
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     296    2046 
   Size:        296    1750
 
BsrDI GCAATG_nn'

Cuts at:      0    1008    2046 
   Size:       1008    1038
 
BsrGI T'GTAC_A

Cuts at:      0    1464    2046 
   Size:       1464     582
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1713    2046 
   Size:       1713     333
 
BstYI r'GATC_y

Cuts at:      0     317    1455    1786    2046 
   Size:        317    1138     331     260

  Fragments arranged by size: 

                1138     331     317     260
 
Bsu36I CC'TnA_GG

Cuts at:      0     231    2046 
   Size:        231    1815
 
BtsI GCAGTG_nn'

Cuts at:      0      26    2046 
   Size:         26    2020
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     990    1025    2046 
   Size:        990      35    1021

  Fragments arranged by size: 

                1021     990      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1723    2046 
   Size:       1723     323
 
EarI CTCTTCn'nnn_

Cuts at:      0     269     293     746     914     925    2046 
   Size:        269      24     453     168      11    1121

  Fragments arranged by size: 

                1121     453     269     168      24      11
 
EcoICRI GAG'CTC

Cuts at:      0     527    1451    1871    1955    2046 
   Size:        527     924     420      84      91

  Fragments arranged by size: 

                 924     527     420      91      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     133     543     749     837     921    1108    1169 
   Size:        105      28     410     206      88      84     187      61

Cuts at:   1169    1253    1257    1341    1421    1425    1509    1521    1528 
   Size:         84       4      84      80       4      84      12       7

Cuts at:   1528    1589    1593    1673    1761    1845    1887    1929    2046 
   Size:         61       4      80      88      84      42      42     117

  Fragments arranged by size: 

                 410     206     187     117     105      88      88      84
                  84      84      84      84      80      80      61      61
                  42      42      28      12       7       4       4       4
            
 
HaeII r_GCGC'y

Cuts at:      0    1776    2046 
   Size:       1776     270
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     278     329     361     435     467     516 
   Size:        214      32      32      51      32      74      32      49

Cuts at:    516     548     672     684     704     716     852     884    1440 
   Size:         32     124      12      20      12     136      32     556

Cuts at:   1440    1472    1944    1976    2046 
   Size:         32     472      32      70

  Fragments arranged by size: 

                 556     472     214     136     124      74      70      51
                  49      32      32      32      32      32      32      32
                  32      20      12      12
 
KpnI G_GTAC'C

Cuts at:      0     288    2046 
   Size:        288    1758
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     374    1426    1930    2046 
   Size:        374    1052     504     116

  Fragments arranged by size: 

                1052     504     374     116
 
MslI CAynn'nnrTG

Cuts at:      0     476     996    1164    1248    1584    1752    1836    1920 
   Size:        476     520     168      84     336     168      84      84

Cuts at:   1920    1988    2046 
   Size:         68      58

  Fragments arranged by size: 

                 520     476     336     168     168      84      84      84
                  68      58
 
MspA1I CmG'CkG

Cuts at:      0     521    1865    2046 
   Size:        521    1344     181

  Fragments arranged by size: 

                1344     521     181
 
NsiI A_TGCA'T

Cuts at:      0    1008    1260    2046 
   Size:       1008     252     786

  Fragments arranged by size: 

                1008     786     252
 
NspI r_CATG'y

Cuts at:      0    1010    1262    1430    2046 
   Size:       1010     252     168     616

  Fragments arranged by size: 

                1010     616     252     168
 
PciI A'CATG_T

Cuts at:      0    1426    2046 
   Size:       1426     620
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     429     461    2046 
   Size:        429      32    1585

  Fragments arranged by size: 

                1585     429      32
 
PstI C_TGCA'G

Cuts at:      0     959    2046 
   Size:        959    1087
 
PvuII CAG'CTG

Cuts at:      0     521    1865    2046 
   Size:        521    1344     181

  Fragments arranged by size: 

                1344     521     181
 
SacI G_AGCT'C

Cuts at:      0     529    1453    1873    1957    2046 
   Size:        529     924     420      84      89

  Fragments arranged by size: 

                 924     529     420      89      84
 
SapI GCTCTTCn'nnn_

Cuts at:      0     293     925    2046 
   Size:        293     632    1121

  Fragments arranged by size: 

                1121     632     293
 
SfcI C'TryA_G

Cuts at:      0     955    1470    2046 
   Size:        955     515     576

  Fragments arranged by size: 

                 955     576     515
 
SmlI C'TyrA_G

Cuts at:      0    1956    2046 
   Size:       1956      90
 
SspI AAT'ATT

Cuts at:      0     372    2046 
   Size:        372    1674
 
StuI AGG'CCT

Cuts at:      0    1366    1522    2046 
   Size:       1366     156     524

  Fragments arranged by size: 

                1366     524     156
 
TatI w'GTAC_w

Cuts at:      0    1464    2046 
   Size:       1464     582
 
Tth111I GACn'n_nGTC

Cuts at:      0       6    2046 
   Size:          6    2040
 
XmnI GAAnn'nnTTC

Cuts at:      0     514     934    1102    1186    1438    1606    1690    1858 
   Size:        514     420     168      84     252     168      84     168

Cuts at:   1858    1942    2046 
   Size:         84     104

  Fragments arranged by size: 

                 514     420     252     168     168     168     104      84
                  84      84
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI   AflIII     AleI     BanI    BanII     BbsI
    BfrBI    BglII    BmgBI     BpmI    BpuEI     BsaI    BsaWI    BsaXI
    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
    BstXI    BstYI   Bsu36I     BtsI    CspCI   DraIII     EarI  EcoICRI
  Eco57MI    HaeII    Hin4I     KpnI     MmeI     MslI   MspA1I     NsiI
     NspI     PciI     PsrI     PstI    PvuII     SacI     SapI     SfcI
     SmlI     SspI     StuI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BsaHI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I     BtgI    BtgZI     ClaI     DraI     DrdI     EaeI     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StyI     SwaI    TaqII
     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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