Restriction map for pF1KB6248
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NarI        NciI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccIII        989  TCCGGA               T^CCGGA
AcyI          766  GACGCC               G[GA]^CG[CT]C
AfaI         1347  GTAC                 GT^AC
AflIII        619  ACGTGT               A^C[GA][CT]GT
AgeI         1935  ACCGGT               A^CCGGT
AvaI          843  CTCGGG               C^[CT]CG[GA]G
AvaII         198  GGTCC                G^G[AT]CC
AviII        1073  TGCGCA               TGC^GCA
BamHI         668  GGATCC               G^GATCC
BanII          33  GGGCTC               G[GA]GC[CT]^C
BbsI         1939  GTCTTC               GAAGAC(2/6)
BciVI        1088  GTATCC               GTATCC(6/5)
BfaI         1207  CTAG                 C^TAG
BglII         701  AGATCT               A^GATCT
BpuAI        1939  GTCTTC               GAAGAC(2/6)
BsaHI         766  GACGCC               G[GA]^CG[CT]C
BsaI          268  GAGACC               GGTCTC(1/5)
BseAI         989  TCCGGA               T^CCGGA
BsiEI        1101  CGACCG               CG[GA][CT]^CG
BsmI         1039  GAATGC               GAATGC(1/-1)
BsoBI         843  CTCGGG               C^[CT]CG[GA]G
BspEI         989  TCCGGA               T^CCGGA
DraI          495  TTTAAA               TTT^AAA
EcoNI        1626  CCTGCGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1073  TGCGCA               TGC^GCA
HgaI          766  GACGC                GACGC(5/10)
Hin1I         766  GACGCC               G[GA]^CG[CT]C
MaeI         1207  CTAG                 C^TAG
MroI          989  TCCGGA               T^CCGGA
NcoI         1179  CCATGG               C^CATGG
PinAI        1935  ACCGGT               A^CCGGT
PvuII         163  CAGCTG               CAG^CTG
RsaI         1347  GTAC                 GT^AC
SmlI          904  CTCAAG               C^T[CT][GA]AG
StuI         1845  AGGCCT               AGG^CCT
TfiI         1860  GATTC                G^A[AT]TC
XbaI         1206  TCTAGA               T^CTAGA
XcmI         1782  CCAGGAAGGCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I        838  GTGCAC               G^TGCAC
             1006  GTGCAC              
AocI          663  CCTGAGG              CC^T[ACGT]AGG
             1769  CCTCAGG             
ApaLI         838  GTGCAC               G^TGCAC
             1006  GTGCAC              
BalI         1247  TGGCCA               TGG^CCA
             1919  TGGCCA              
BglI          587  GCCACACGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1573  GCCTTGGTGGC         
BsaWI         989  TCCGGA               [AT]^CCGG[AT]
             1935  ACCGGT              
BseRI        1944  CTCCTC               GAGGAG(10/8)
             1947  CTCCTC              
BsrBI         487  GAGCGG               CCGCTC(-3/-3)
              682  GAGCGG              
BssSI        1081  CACGAG               CACGAG(-5/-1)
             1720  CACGAG              
Bsu36I        663  CCTGAGG              CC^T[ACGT]AGG
             1769  CCTCAGG             
EaeI         1247  TGGCCA               [CT]^GGCC[GA]
             1919  TGGCCA              
Eco81I        663  CCTGAGG              CC^T[ACGT]AGG
             1769  CCTCAGG             
EcoT14I      1179  CCATGG               C^C[AT][AT]GG
             1574  CCTTGG              
HaeII         793  AGCGCC               [GA]GCGC^[CT]
             1929  AGCGCC              
MaeII         619  ACGT                 A^CGT
             1370  ACGT                
MluNI        1247  TGGCCA               TGG^CCA
             1919  TGGCCA              
MscI         1247  TGGCCA               TGG^CCA
             1919  TGGCCA              
NspI          578  ACATGC               [GA]CATG^[CT]
              830  ACATGC              
StyI         1179  CCATGG               C^C[AT][AT]GG
             1574  CCTTGG              
TaqI         1133  TCGA                 T^CGA
             1394  TCGA                
TthHB8I      1133  TCGA                 T^CGA
             1394  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       BanI        
BbvI        BmyI        BpmI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DsaI        EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       XmnI        
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