Restriction map for pF1KB6248
 (Linear) (Six-base) MAPSORT of: KIBB6248.seq  Check: 7475  from: 1  to: 2043



KIBB6248.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     423     755    1233    2043 
   Size:        423     332     478     810

  Fragments arranged by size: 

                 810     478     423     332
 
AflIII A'CryG_T

Cuts at:      0     619    2043 
   Size:        619    1424
 
AgeI A'CCGG_T

Cuts at:      0    1935    2043 
   Size:       1935     108
 
AleI CACnn'nnGTG

Cuts at:      0      61     595    2043 
   Size:         61     534    1448

  Fragments arranged by size: 

                1448     534      61
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      79    2043 
   Size:         79    1964
 
AlwNI CAG_nnn'CTG

Cuts at:      0     226     364    1626    2043 
   Size:        226     138    1262     417

  Fragments arranged by size: 

                1262     417     226     138
 
ApaLI G'TGCA_C

Cuts at:      0     838    1006    2043 
   Size:        838     168    1037

  Fragments arranged by size: 

                1037     838     168
 
ApoI r'AATT_y

Cuts at:      0      44     298     500    2043 
   Size:         44     254     202    1543

  Fragments arranged by size: 

                1543     254     202      44
 
AvaI C'yCGr_G

Cuts at:      0     843    2043 
   Size:        843    1200
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     903     936    1071    1104    2043 
   Size:        903      33     135      33     939

  Fragments arranged by size: 

                 939     903     135      33      33
 
BamHI G'GATC_C

Cuts at:      0     668    2043 
   Size:        668    1375
 
BanI G'GyrC_C

Cuts at:      0     584    1443    1512    2043 
   Size:        584     859      69     531

  Fragments arranged by size: 

                 859     584     531      69
 
BanII G_rGCy'C

Cuts at:      0      37    2043 
   Size:         37    2006
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1932    2043 
   Size:       1932     111
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1099    2043 
   Size:       1099     944
 
BglI GCCn_nnn'nGGC

Cuts at:      0     593    1579    2043 
   Size:        593     986     464

  Fragments arranged by size: 

                 986     593     464
 
BglII A'GATC_T

Cuts at:      0     701    2043 
   Size:        701    1342
 
Bme1580I G_kGCm'C

Cuts at:      0     842    1010    1448    1469    1517    2043 
   Size:        842     168     438      21      48     526

  Fragments arranged by size: 

                 842     526     438     168      48      21
 
BmgBI CAC'GTC

Cuts at:      0     620    1371    2043 
   Size:        620     751     672

  Fragments arranged by size: 

                 751     672     620
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1464    2043 
   Size:       1464     579
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1050    2043 
   Size:       1050     993
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     711    1041    1366    1423    1435    2043 
   Size:        278     433     330     325      57      12     608

  Fragments arranged by size: 

                 608     433     330     325     278      57      12
 
Bpu10I CC'TnA_GC

Cuts at:      0    1156    2043 
   Size:       1156     887
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889    2043 
   Size:        889    1154
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     262    2043 
   Size:        262    1781
 
BsaHI Gr'CG_yC

Cuts at:      0     767    2043 
   Size:        767    1276
 
BsaWI w'CCGG_w

Cuts at:      0     989    1935    2043 
   Size:        989     946     108

  Fragments arranged by size: 

                 989     946     108
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1039    1069    2043 
   Size:       1039      30     974

  Fragments arranged by size: 

                1039     974      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1935    1938    2043 
   Size:       1935       3     105

  Fragments arranged by size: 

                1935     105       3
 
BseYI C'CCAG_C

Cuts at:      0     190    1147    1399    1456    1694    1955    2043 
   Size:        190     957     252      57     238     261      88

  Fragments arranged by size: 

                 957     261     252     238     190      88      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     870    1080    1729    2043 
   Size:        870     210     649     314

  Fragments arranged by size: 

                 870     649     314     210
 
BsiEI CG_ry'CG

Cuts at:      0    1104    2043 
   Size:       1104     939
 
BsiHKAI G_wGCw'C

Cuts at:      0     842    1010    1036    2043 
   Size:        842     168      26    1007

  Fragments arranged by size: 

                1007     842     168      26
 
BsmI GAATG_Cn'

Cuts at:      0    1045    2043 
   Size:       1045     998
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     842    1010    1036    1448    1469    1517    2043 
   Size:         37     805     168      26     412      21      48     526

  Fragments arranged by size: 

                 805     526     412     168      48      37      26      21
            
 
BspEI T'CCGG_A

Cuts at:      0     989    2043 
   Size:        989    1054
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     338     423     755    1233    2043 
   Size:        338      85     332     478     810

  Fragments arranged by size: 

                 810     478     338     332      85
 
BsrBI CCG'CTC

Cuts at:      0     489     684    2043 
   Size:        489     195    1359

  Fragments arranged by size: 

                1359     489     195
 
BsrDI GCAATG_nn'

Cuts at:      0     882     954    1694    2043 
   Size:        882      72     740     349

  Fragments arranged by size: 

                 882     740     349      72
 
BsrFI r'CCGG_y

Cuts at:      0    1222    1238    1613    1698    1935    2043 
   Size:       1222      16     375      85     237     108

  Fragments arranged by size: 

                1222     375     237     108      85      16
 
BssSI C'ACGA_G

Cuts at:      0    1081    1720    2043 
   Size:       1081     639     323

  Fragments arranged by size: 

                1081     639     323
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1124    1512    1626    2043 
   Size:       1124     388     114     417

  Fragments arranged by size: 

                1124     417     388     114
 
BstYI r'GATC_y

Cuts at:      0     668     701    1622    2043 
   Size:        668      33     921     421

  Fragments arranged by size: 

                 921     668     421      33
 
Bsu36I CC'TnA_GG

Cuts at:      0     664    1770    2043 
   Size:        664    1106     273

  Fragments arranged by size: 

                1106     664     273
 
BtgI C'CryG_G

Cuts at:      0     335    1179    1763    2043 
   Size:        335     844     584     280

  Fragments arranged by size: 

                 844     584     335     280
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1304    1896    1910    2043 
   Size:       1304     592      14     133

  Fragments arranged by size: 

                1304     592     133      14
 
BtsI GCAGTG_nn'

Cuts at:      0    1092    1275    1559    1922    2043 
   Size:       1092     183     284     363     121

  Fragments arranged by size: 

                1092     363     284     183     121
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     135     170    2043 
   Size:        135      35    1873

  Fragments arranged by size: 

                1873     135      35
 
DraI TTT'AAA

Cuts at:      0     497    2043 
   Size:        497    1546
 
EaeI y'GGCC_r

Cuts at:      0    1247    1919    2043 
   Size:       1247     672     124

  Fragments arranged by size: 

                1247     672     124
 
EarI CTCTTCn'nnn_

Cuts at:      0    1261    1479    1956    2043 
   Size:       1261     218     477      87

  Fragments arranged by size: 

                1261     477     218      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     711    1041    1366    1423    1435    2043 
   Size:        278     433     330     325      57      12     608

  Fragments arranged by size: 

                 608     433     330     325     278      57      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1630    2043 
   Size:       1630     413
 
FspI TGC'GCA

Cuts at:      0    1075    2043 
   Size:       1075     968
 
HaeII r_GCGC'y

Cuts at:      0     797    1933    2043 
   Size:        797    1136     110

  Fragments arranged by size: 

                1136     797     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1188    1220    1478    1510    2043 
   Size:       1188      32     258      32     533

  Fragments arranged by size: 

                1188     533     258      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     995    2043 
   Size:        995    1048
 
MscI TGG'CCA

Cuts at:      0    1249    1921    2043 
   Size:       1249     672     122

  Fragments arranged by size: 

                1249     672     122
 
MslI CAynn'nnrTG

Cuts at:      0      61     527     595     835    1915    2043 
   Size:         61     466      68     240    1080     128

  Fragments arranged by size: 

                1080     466     240     128      68      61
 
MspA1I CmG'CkG

Cuts at:      0     165     634     884     932    1549    2043 
   Size:        165     469     250      48     617     494

  Fragments arranged by size: 

                 617     494     469     250     165      48
 
NaeI GCC'GGC

Cuts at:      0    1240    1615    1700    2043 
   Size:       1240     375      85     343

  Fragments arranged by size: 

                1240     375     343      85
 
NcoI C'CATG_G

Cuts at:      0    1179    2043 
   Size:       1179     864
 
NgoMIV G'CCGG_C

Cuts at:      0    1238    1613    1698    2043 
   Size:       1238     375      85     345

  Fragments arranged by size: 

                1238     375     345      85
 
NspI r_CATG'y

Cuts at:      0     582     834    2043 
   Size:        582     252    1209

  Fragments arranged by size: 

                1209     582     252
 
PasI CC'CwG_GG

Cuts at:      0    1603    2043 
   Size:       1603     440
 
PstI C_TGCA'G

Cuts at:      0    1129    1554    1622    2043 
   Size:       1129     425      68     421

  Fragments arranged by size: 

                1129     425     421      68
 
PvuII CAG'CTG

Cuts at:      0     165    2043 
   Size:        165    1878
 
SfcI C'TryA_G

Cuts at:      0     789    1125    1550    1618    2043 
   Size:        789     336     425      68     425

  Fragments arranged by size: 

                 789     425     425     336      68
 
SmlI C'TyrA_G

Cuts at:      0     904    2043 
   Size:        904    1139
 
StuI AGG'CCT

Cuts at:      0    1847    2043 
   Size:       1847     196
 
StyI C'CwwG_G

Cuts at:      0    1179    1574    2043 
   Size:       1179     395     469

  Fragments arranged by size: 

                1179     469     395
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     247     958    1732    2043 
   Size:        247     711     774     311

  Fragments arranged by size: 

                 774     711     311     247
 
TatI w'GTAC_w

Cuts at:      0    1346    2043 
   Size:       1346     697
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     736     768    2043 
   Size:        736      32    1275

  Fragments arranged by size: 

                1275     736      32
 
XbaI T'CTAG_A

Cuts at:      0    1206    2043 
   Size:       1206     837
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1789    2043 
   Size:       1789     254
 
XmnI GAAnn'nnTTC

Cuts at:      0     856     940    1174    1270    2043 
   Size:        856      84     234      96     773

  Fragments arranged by size: 

                 856     773     234      96      84
 

 Enzymes that do cut:

     AarI   AflIII     AgeI     AleI     AlfI    AlwNI    ApaLI     ApoI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BciVI     BglI
    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI     EaeI     EarI  Eco57MI    EcoNI     FspI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI     PstI    PvuII     SfcI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AhdI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BbeI    BbvCI
     BcgI     BclI    BfrBI     BlpI     BmtI    BsaAI    BsaBI    BsiWI
    BsmBI    BspHI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I
     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XhoI     XmaI     ZraI
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