Restriction map for
pF1KB6245
(Circular) (Six-base) MAPSORT of: pF1KB6245.seq Check: 9657 from: 1 to: 5372
pF1KB6245 5372 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 312 327 1515 1536 312
Size: 15 1188 21 4148
Fragments arranged by size:
4148 1188 21 15
AccI GT'mk_AC
Cuts at: 1611 1880 2410 1611
Size: 269 530 4573
Fragments arranged by size:
4573 530 269
Acc65I G'GTAC_C
Cuts at: 1384 2072 2388 1384
Size: 688 316 4368
Fragments arranged by size:
4368 688 316
AclI AA'CG_TT
Cuts at: 5212 5212
Size: 5372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1077 1706 3197 3629 4376 4644 932
Size: 145 629 1491 432 747 268 1660
Fragments arranged by size:
1660 1491 747 629 432 268 145
AflIII A'CryG_T
Cuts at: 1875 2724 3828 4805 1875
Size: 849 1104 977 2442
Fragments arranged by size:
2442 1104 977 849
AgeI A'CCGG_T
Cuts at: 580 3760 580
Size: 3180 2192
AlwNI CAG_nnn'CTG
Cuts at: 498 531 2677 4244 498
Size: 33 2146 1567 1626
Fragments arranged by size:
2146 1626 1567 33
ApaI G_GGCC'C
Cuts at: 539 921 539
Size: 382 4990
ApaLI G'TGCA_C
Cuts at: 4142 4142
Size: 5372
ApoI r'AATT_y
Cuts at: 1306 2025 2157 2376 4725 4981 1306
Size: 719 132 219 2349 256 1697
Fragments arranged by size:
2349 1697 719 256 219 132
AseI AT'TA_AT
Cuts at: 20 20
Size: 5372
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
AvaI C'yCGr_G
Cuts at: 124 475 563 619 757 769 2392 124
Size: 351 88 56 138 12 1623 3104
Fragments arranged by size:
3104 1623 351 138 88 56 12
AvrII C'CTAG_G
Cuts at: 1801 1801
Size: 5372
BamHI G'GATC_C
Cuts at: 884 923 1154 1602 2397 884
Size: 39 231 448 795 3859
Fragments arranged by size:
3859 795 448 231 39
BanI G'GyrC_C
Cuts at: 184 460 853 1384 2072 2388 2802 3050 3085
Size: 276 393 531 688 316 414 248 35
Cuts at: 3085 184
Size: 2471
Fragments arranged by size:
2471 688 531 414 393 316 276 248
35
BanII G_rGCy'C
Cuts at: 215 539 797 921 942 1543 2386 3416 215
Size: 324 258 124 21 601 843 1030 2171
Fragments arranged by size:
2171 1030 843 601 324 258 124 21
BbeI G_GCGC'C
Cuts at: 464 2806 3054 464
Size: 2342 248 2782
Fragments arranged by size:
2782 2342 248
BbsI GAAGACnn'nnnn_
Cuts at: 2072 2265 2072
Size: 193 5179
BbvCI CC'TCA_GC
Cuts at: 137 2243 137
Size: 2106 3266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2022 2056 2022
Size: 34 5338
BciVI GTATCCnnnnn_n'
Cuts at: 3270 4031 5372 3270
Size: 761 1341 3270
Fragments arranged by size:
3270 1341 761
BclI T'GATC_A
Cuts at: 740 2892 740
Size: 2152 3220
BglII A'GATC_T
Cuts at: 2887 4924 2887
Size: 2037 3335
BlpI GC'TnA_GC
Cuts at: 2484 2484
Size: 5372
Bme1580I G_kGCm'C
Cuts at: 187 539 921 2997 3090 4146 187
Size: 352 382 2076 93 1056 1413
Fragments arranged by size:
2076 1413 1056 382 352 93
BmrI ACTGGGnnnn_n'
Cuts at: 1658 2584 2665 3000 5144 1658
Size: 926 81 335 2144 1886
Fragments arranged by size:
2144 1886 926 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 3739 204
Size: 3535 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 579 685 1033 1560 453
Size: 126 106 348 527 4265
Fragments arranged by size:
4265 527 348 126 106
Bpu10I CC'TnA_GC
Cuts at: 137 1586 2243 2356 137
Size: 1449 657 113 3153
Fragments arranged by size:
3153 1449 657 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2118 2548 3427 3919 4217 2118
Size: 430 879 492 298 3273
Fragments arranged by size:
3273 879 492 430 298
BsaI GGTCTCn'nnnn_
Cuts at: 5076 5076
Size: 5372
BsaAI yAC'GTr
Cuts at: 1445 2725 1445
Size: 1280 4092
BsaBI GATnn'nnATC
Cuts at: 6 2886 4923 6
Size: 2880 2037 455
Fragments arranged by size:
2880 2037 455
BsaHI Gr'CG_yC
Cuts at: 286 461 2803 3051 3753 5249 286
Size: 175 2342 248 702 1496 409
Fragments arranged by size:
2342 1496 702 409 248 175
BsaWI w'CCGG_w
Cuts at: 580 850 2561 3082 3760 4034 4181 4592 580
Size: 270 1711 521 678 274 147 411 1360
Fragments arranged by size:
1711 1360 678 521 411 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 784 814 2277 2307 784
Size: 30 1463 30 3849
Fragments arranged by size:
3849 1463 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 688 757 2350 688
Size: 69 1593 3710
Fragments arranged by size:
3710 1593 69
BseYI C'CCAG_C
Cuts at: 531 538 1185 1462 1567 2797 3555 4132 4628
Size: 7 647 277 105 1230 758 577 496
Cuts at: 4628 531
Size: 1275
Fragments arranged by size:
1275 1230 758 647 577 496 277 105
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 5372
BsiEI CG_ry'CG
Cuts at: 104 179 2914 2960 4168 104
Size: 75 2735 46 1208 1308
Fragments arranged by size:
2735 1308 1208 75 46
BsiHKAI G_wGCw'C
Cuts at: 942 1842 2357 2386 3164 3354 4146 942
Size: 900 515 29 778 190 792 2168
Fragments arranged by size:
2168 900 792 778 515 190 29
BsmI GAATG_Cn'
Cuts at: 1680 2053 2146 1680
Size: 373 93 4906
Fragments arranged by size:
4906 373 93
BsmBI CGTCTCn'nnnn_
Cuts at: 230 1578 230
Size: 1348 4024
Bsp1286I G_dGCh'C
Cuts at: 187 215 539 797 921 942 1543 1842 2357
Size: 28 324 258 124 21 601 299 515
Cuts at: 2357 2386 2997 3090 3164 3354 3416 4146 187
Size: 29 611 93 74 190 62 730 1413
Fragments arranged by size:
1413 730 611 601 515 324 299 258
190 124 93 74 62 29 28 21
BspEI T'CCGG_A
Cuts at: 4592 4592
Size: 5372
BspHI T'CATG_A
Cuts at: 680 4548 680
Size: 3868 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 312 327 434 1515 1536 2422 2636 2938 3319
Size: 15 107 1081 21 886 214 302 381
Cuts at: 3319 312
Size: 2365
Fragments arranged by size:
2365 1081 886 381 302 214 107 21
15
BsrBI CCG'CTC
Cuts at: 50 160 1050 1436 3664 3718 5202 5367 50
Size: 110 890 386 2228 54 1484 165 55
Fragments arranged by size:
2228 1484 890 386 165 110 55 54
BsrDI GCAATG_nn'
Cuts at: 3284 3284
Size: 5372
BsrFI r'CCGG_y
Cuts at: 174 580 624 3370 3551 3760 174
Size: 406 44 2746 181 209 1786
Fragments arranged by size:
2746 1786 406 209 181 44
BssSI C'ACGA_G
Cuts at: 3643 4001 3643
Size: 358 5014
BstAPI GCAn_nnn'nTGC
Cuts at: 250 342 489 1762 2749 250
Size: 92 147 1273 987 2873
Fragments arranged by size:
2873 1273 987 147 92
BstBI TT'CG_AA
Cuts at: 3733 3733
Size: 5372
BstXI CCAn_nnnn'nTGG
Cuts at: 810 810
Size: 5372
BstYI r'GATC_y
Cuts at: 1 884 923 1154 1602 2397 2864 2887 3222
Size: 883 39 231 448 795 467 23 335
Cuts at: 3222 3468 4480 4566 4578 4924 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 883 795 467 449 448 346 335
246 231 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 359 880 359
Size: 521 4851
BtgI C'CryG_G
Cuts at: 107 206 302 107
Size: 99 96 5177
Fragments arranged by size:
5177 99 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3322 3503 3666 3322
Size: 181 163 5028
Fragments arranged by size:
5028 181 163
BtsI GCAGTG_nn'
Cuts at: 287 2615 287
Size: 2328 3044
DraI TTT'AAA
Cuts at: 2105 2371 2105
Size: 266 5106
DraIII CAC_nnn'GTG
Cuts at: 489 1701 2152 489
Size: 1212 451 3709
Fragments arranged by size:
3709 1212 451
DrdI GACnn_nn'nnGTC
Cuts at: 3078 3936 3078
Size: 858 4514
EaeI y'GGCC_r
Cuts at: 1899 2957 3131 3522 3549 4894 1899
Size: 1058 174 391 27 1345 2377
Fragments arranged by size:
2377 1345 1058 391 174 27
EagI C'GGCC_G
Cuts at: 2957 2957
Size: 5372
EarI CTCTTCn'nnn_
Cuts at: 3395 3605 3395
Size: 210 5162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 152 685 932 3580 3890 4036 5182 152
Size: 533 247 2648 310 146 1146 342
Fragments arranged by size:
2648 1146 533 342 310 247 146
EcoICRI GAG'CTC
Cuts at: 940 2384 940
Size: 1444 3928
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 579 685 932 1033 1077 1560 1706 3197
Size: 126 106 247 101 44 483 146 1491
Cuts at: 3197 3629 4376 4644 453
Size: 432 747 268 1181
Fragments arranged by size:
1491 1181 747 483 432 268 247 146
126 106 101 44
EcoNI CCTnn'n_nnAGG
Cuts at: 1334 1334
Size: 5372
EcoO109I rG'GnC_Cy
Cuts at: 535 559 616 917 972 2511 535
Size: 24 57 301 55 1539 3396
Fragments arranged by size:
3396 1539 301 57 55 24
EcoRI G'AATT_C
Cuts at: 2376 2376
Size: 5372
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1955 3391 1955
Size: 1436 3936
FseI GG_CCGG'CC
Cuts at: 628 628
Size: 5372
FspI TGC'GCA
Cuts at: 3153 3153
Size: 5372
HaeII r_GCGC'y
Cuts at: 464 505 1736 2806 3054 4076 5055 464
Size: 41 1231 1070 248 1022 979 781
Fragments arranged by size:
1231 1070 1022 979 781 248 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 557 589 784 816 2277 2309 3214 3244 3246
Size: 32 195 32 1461 32 905 30 2
Cuts at: 3246 3276 557
Size: 30 2653
Fragments arranged by size:
2653 1461 905 195 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2411 2411
Size: 5372
KasI G'GCGC_C
Cuts at: 460 2802 3050 460
Size: 2342 248 2782
Fragments arranged by size:
2782 2342 248
KpnI G_GTAC'C
Cuts at: 1388 2076 2392 1388
Size: 688 316 4368
Fragments arranged by size:
4368 688 316
MluI A'CGCG_T
Cuts at: 4805 4805
Size: 5372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 1818 4043 4227 590
Size: 1228 2225 184 1735
Fragments arranged by size:
2225 1735 1228 184
MscI TGG'CCA
Cuts at: 1901 3133 1901
Size: 1232 4140
MslI CAynn'nnrTG
Cuts at: 1874 3361 3488 1874
Size: 1487 127 3758
Fragments arranged by size:
3758 1487 127
MspA1I CmG'CkG
Cuts at: 171 304 382 1518 1677 2247 2483 2797 3157
Size: 133 78 1136 159 570 236 314 360
Cuts at: 3157 4170 4415 171
Size: 1013 245 1128
Fragments arranged by size:
1136 1128 1013 570 360 314 245 236
159 133 78
NaeI GCC'GGC
Cuts at: 626 3553 626
Size: 2927 2445
NarI GG'CG_CC
Cuts at: 461 2803 3051 461
Size: 2342 248 2782
Fragments arranged by size:
2782 2342 248
NcoI C'CATG_G
Cuts at: 107 206 107
Size: 99 5273
NgoMIV G'CCGG_C
Cuts at: 624 3551 624
Size: 2927 2445
NspI r_CATG'y
Cuts at: 511 1879 1994 2425 2925 3832 511
Size: 1368 115 431 500 907 2051
Fragments arranged by size:
2051 1368 907 500 431 115
PasI CC'CwG_GG
Cuts at: 913 913
Size: 5372
PciI A'CATG_T
Cuts at: 1875 3828 1875
Size: 1953 3419
PfoI T'CCnGG_A
Cuts at: 1598 1598
Size: 5372
PmeI GTTT'AAAC
Cuts at: 2371 2371
Size: 5372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 4511
Size: 32 5340
PpuMI rG'GwC_Cy
Cuts at: 972 972
Size: 5372
PshAI GACnn'nnGTC
Cuts at: 970 970
Size: 5372
PspOMI G'GGCC_C
Cuts at: 535 917 535
Size: 382 4990
PspXI vC'TCGA_Gb
Cuts at: 563 769 563
Size: 206 5166
PstI C_TGCA'G
Cuts at: 323 440 781 1533 2419 3104 323
Size: 117 341 752 886 685 2591
Fragments arranged by size:
2591 886 752 685 341 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5372
PvuII CAG'CTG
Cuts at: 1518 1677 2247 2797 3157 1518
Size: 159 570 550 360 3733
Fragments arranged by size:
3733 570 550 360 159
RsrII CG'GwC_CG
Cuts at: 3567 3567
Size: 5372
SacI G_AGCT'C
Cuts at: 942 2386 942
Size: 1444 3928
SacII CC_GC'GG
Cuts at: 305 305
Size: 5372
SalI G'TCGA_C
Cuts at: 2409 2409
Size: 5372
SapI GCTCTTCn'nnn_
Cuts at: 3395 3605 3395
Size: 210 5162
SbfI CC_TGCA'GG
Cuts at: 2419 2419
Size: 5372
ScaI AGT'ACT
Cuts at: 1761 4889 1761
Size: 3128 2244
SfcI C'TryA_G
Cuts at: 33 319 436 777 1468 1529 2415 3100 4093
Size: 286 117 341 691 61 886 685 993
Cuts at: 4093 4284 33
Size: 191 1121
Fragments arranged by size:
1121 993 886 691 685 341 286 191
117 61
SfoI GGC'GCC
Cuts at: 462 2804 3052 462
Size: 2342 248 2782
Fragments arranged by size:
2782 2342 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
SmaI CCC'GGG
Cuts at: 621 2394 621
Size: 1773 3599
SmlI C'TyrA_G
Cuts at: 563 769 2133 2527 3442 3934 4196 563
Size: 206 1364 394 915 492 262 1739
Fragments arranged by size:
1739 1364 915 492 394 262 206
SnaBI TAC'GTA
Cuts at: 1445 1445
Size: 5372
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5372
SphI G_CATG'C
Cuts at: 2425 2925 2425
Size: 500 4872
StyI C'CwwG_G
Cuts at: 107 206 257 968 1801 1896 2261 2506 2860
Size: 99 51 711 833 95 365 245 354
Cuts at: 2860 107
Size: 2619
Fragments arranged by size:
2619 833 711 365 354 245 99 95
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1886 2955 3088 3547 3756 1886
Size: 1069 133 459 209 3502
Fragments arranged by size:
3502 1069 459 209 133
TatI w'GTAC_w
Cuts at: 1759 1987 4887 1759
Size: 228 2900 2244
Fragments arranged by size:
2900 2244 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1496 1517 1528 1549 4889 4921 1496
Size: 21 11 21 3340 32 1947
Fragments arranged by size:
3340 1947 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 450 3169 450
Size: 2719 2653
XbaI T'CTAG_A
Cuts at: 1725 2403 1725
Size: 678 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 1645 2867 255
Size: 1390 1222 2760
Fragments arranged by size:
2760 1390 1222
XhoI C'TCGA_G
Cuts at: 563 769 563
Size: 206 5166
XmaI C'CCGG_G
Cuts at: 619 2392 619
Size: 1773 3599
XmnI GAAnn'nnTTC
Cuts at: 2272 2272
Size: 5372
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
BaeI BfrBI BglI BmgBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PsiI PsrI SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI ZraI