Restriction map for pF1KB6730
 (Linear) (Six-base) MAPSORT of: KIBB6730.seq  Check: 694  from: 1  to: 414



KIBB6730.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     174     414 
   Size:        174     240
 
ApaI G_GGCC'C

Cuts at:      0      45     414 
   Size:         45     369
 
BanI G'GyrC_C

Cuts at:      0     134     414 
   Size:        134     280
 
BanII G_rGCy'C

Cuts at:      0      45     286     413     414 
   Size:         45     241     127       1

  Fragments arranged by size: 

                 241     127      45       1
 
BbeI G_GCGC'C

Cuts at:      0     138     414 
   Size:        138     276
 
BbvCI CC'TCA_GC

Cuts at:      0     349     414 
   Size:        349      65
 
Bme1580I G_kGCm'C

Cuts at:      0      45     119     414 
   Size:         45      74     295

  Fragments arranged by size: 

                 295      74      45
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     362     414 
   Size:        362      52
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     414 
   Size:        160     254
 
Bpu10I CC'TnA_GC

Cuts at:      0     349     414 
   Size:        349      65
 
BsaHI Gr'CG_yC

Cuts at:      0      89     135     414 
   Size:         89      46     279

  Fragments arranged by size: 

                 279      89      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      79     109     414 
   Size:         79      30     305

  Fragments arranged by size: 

                 305      79      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     122     157     160     196     414 
   Size:        122      35       3      36     218

  Fragments arranged by size: 

                 218     122      36      35       3
 
BseYI C'CCAG_C

Cuts at:      0      44     261     354     390     394     414 
   Size:         44     217      93      36       4      20

  Fragments arranged by size: 

                 217      93      44      36      20       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     414 
   Size:        202     212
 
BsiHKAI G_wGCw'C

Cuts at:      0     127     413     414 
   Size:        127     286       1

  Fragments arranged by size: 

                 286     127       1
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     119     127     286     413     414 
   Size:         45      74       8     159     127       1

  Fragments arranged by size: 

                 159     127      74      45       8       1
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      18     414 
   Size:         18     396
 
Bsu36I CC'TnA_GG

Cuts at:      0     332     414 
   Size:        332      82
 
BtgI C'CryG_G

Cuts at:      0      55     414 
   Size:         55     359
 
EaeI y'GGCC_r

Cuts at:      0     191     414 
   Size:        191     223
 
EcoICRI GAG'CTC

Cuts at:      0     411     414 
   Size:        411       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     414 
   Size:        160     254
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     104     142     414 
   Size:        104      38     272

  Fragments arranged by size: 

                 272     104      38
 
EcoO109I rG'GnC_Cy

Cuts at:      0      28      41      81     336     414 
   Size:         28      13      40     255      78

  Fragments arranged by size: 

                 255      78      40      28      13
 
FspI TGC'GCA

Cuts at:      0     163     414 
   Size:        163     251
 
HaeII r_GCGC'y

Cuts at:      0      51     138     312     414 
   Size:         51      87     174     102

  Fragments arranged by size: 

                 174     102      87      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     414 
   Size:         79      32     303

  Fragments arranged by size: 

                 303      79      32
 
KasI G'GCGC_C

Cuts at:      0     134     414 
   Size:        134     280
 
MscI TGG'CCA

Cuts at:      0     193     414 
   Size:        193     221
 
MspA1I CmG'CkG

Cuts at:      0      57     394     414 
   Size:         57     337      20

  Fragments arranged by size: 

                 337      57      20
 
NarI GG'CG_CC

Cuts at:      0     135     414 
   Size:        135     279
 
PasI CC'CwG_GG

Cuts at:      0      33     414 
   Size:         33     381
 
PspOMI G'GGCC_C

Cuts at:      0      41     414 
   Size:         41     373
 
PstI C_TGCA'G

Cuts at:      0      11     241     414 
   Size:         11     230     173

  Fragments arranged by size: 

                 230     173      11
 
PvuII CAG'CTG

Cuts at:      0     394     414 
   Size:        394      20
 
SacI G_AGCT'C

Cuts at:      0     413     414 
   Size:        413       1
 
SacII CC_GC'GG

Cuts at:      0      58     414 
   Size:         58     356
 
SfcI C'TryA_G

Cuts at:      0       7     237     414 
   Size:          7     230     177

  Fragments arranged by size: 

                 230     177       7
 
SfoI GGC'GCC

Cuts at:      0     136     414 
   Size:        136     278
 
StyI C'CwwG_G

Cuts at:      0      65     414 
   Size:         65     349
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     211     414 
   Size:        211     203
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     354     386     414 
   Size:        354      32      28

  Fragments arranged by size: 

                 354      32      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     135     414 
   Size:        135     279
 

 Enzymes that do cut:

    AlwNI     ApaI     BanI    BanII     BbeI    BbvCI Bme1580I     BmrI
     BpmI   Bpu10I    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I   BstAPI   Bsu36I     BtgI     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FspI    HaeII    Hin4I     KasI     MscI   MspA1I     NarI
     PasI   PspOMI     PstI    PvuII     SacI    SacII     SfcI     SfoI
     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoRI    EcoRV     FalI     FseI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com