Restriction map for pF1KB6205
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        MfeI        
MluI        MroI        MslI        MunI        NaeI        
NarI        NgoMIV      NotI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1307  GTCTAC               GT^[AC][GT]AC
AocI         1147  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1438  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1568  CTCGGG               C^[CT]CG[GA]G
AvaII         969  GGACC                G^G[AT]CC
BamHI        1860  GGATCC               G^GATCC
BanI           91  GGCACC               G^G[CT][GA]CC
BclI         1386  TGATCA               T^GATCA
BcnI          509  CCCGG                CC^[GC]GG
BglII        1127  AGATCT               A^GATCT
BsaBI        1951  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI         747  GTCCC                GGGAC(10/14)
BsmI          704  GCATTC               GAATGC(1/-1)
BsoBI        1568  CTCGGG               C^[CT]CG[GA]G
BsrBI        1178  CCGCTC               CCGCTC(-3/-3)
BsrFI        1010  ACCGGC               [GA]^CCGG[CT]
Bsu36I       1147  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1010  ACCGGC               [GA]^CCGG[CT]
DraI          384  TTTAAA               TTT^AAA
DraII         968  AGGACCT              [GA]G^G[ACGT]CC[CT]
EarI         1090  GAAGAG               CTCTTC(1/4)
Eco81I       1147  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      968  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1378  GAATTC               G^AATTC
FbaI         1386  TGATCA               T^GATCA
HaeII        1013  GGCGCT               [GA]GCGC^[CT]
HgaI          137  GCGTC                GACGC(5/10)
Ksp632I      1090  GAAGAG               CTCTTC(1/4)
KspI          503  CCGCGG               CCGC^GG
MaeII         660  ACGT                 A^CGT
MamI         1951  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI          509  CCCGG                CC^[GC]GG
NcoI          612  CCATGG               C^CATGG
NdeI          200  CATATG               CA^TATG
NheI          406  GCTAGC               G^CTAGC
NruI         1157  TCGCGA               TCG^CGA
PpuMI         968  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         480  GACCGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          562  CTGCAG               CTGCA^G
PvuII         115  CAGCTG               CAG^CTG
SacII         503  CCGCGG               CCGC^GG
ScaI          539  AGTACT               AGT^ACT
SmlI         1652  CTCAAG               C^T[CT][GA]AG
TaqI         1355  TCGA                 T^CGA
TthHB8I      1355  TCGA                 T^CGA
XcmI          938  CCAGGGGAGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1438  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI         1254  AAATTC               [GA]^AATT[CT]
             1378  GAATTC              
ApoI         1254  AAATTC               [GA]^AATT[CT]
             1378  GAATTC              
AseI         1825  ATTAAT               AT^TAAT
             1881  ATTAAT              
AsnI         1825  ATTAAT               AT^TAAT
             1881  ATTAAT              
BalI          167  TGGCCA               TGG^CCA
              581  TGGCCA              
BanII         146  GAGCCC               G[GA]GC[CT]^C
              592  GAGCCC              
BbsI          477  GAAGAC               GAAGAC(2/6)
             1006  GAAGAC              
BfaI           25  CTAG                 C^TAG
              407  CTAG                
BpuAI         477  GAAGAC               GAAGAC(2/6)
             1006  GAAGAC              
BsmAI        1551  GAGAC                GTCTC(1/5)
             1719  GAGAC               
BsrDI         127  GCAATG               GCAATG(2/0)
              410  GCAATG              
EcoNI          21  CCTGCTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              887  CCTGGGAGAGG         
EcoT14I       612  CCATGG               C^C[AT][AT]GG
              912  CCAAGG              
HapII         510  CCGG                 C^CGG
             1011  CCGG                
HpaII         510  CCGG                 C^CGG
             1011  CCGG                
MaeI           25  CTAG                 C^TAG
              407  CTAG                
MluNI         167  TGGCCA               TGG^CCA
              581  TGGCCA              
MscI          167  TGGCCA               TGG^CCA
              581  TGGCCA              
MspA1I        115  CAGCTG               C[AC]G^C[GT]G
              503  CCGCGG              
MspI          510  CCGG                 C^CGG
             1011  CCGG                
PshBI        1825  ATTAAT               AT^TAAT
             1881  ATTAAT              
SfaNI          85  GATGC                GCATC(5/9)
              738  GATGC               
SfcI          562  CTGCAG               C^T[GA][CT]AG
             1373  CTACAG              
StyI          612  CCATGG               C^C[AT][AT]GG
              912  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoO65I     EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XhoII       
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