Restriction map for pF1KB6205
 (Linear) (Six-base) MAPSORT of: KIBB6205.seq  Check: 3965  from: 1  to: 1992



KIBB6205.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:      0    1308    1992 
   Size:       1308     684
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    1334    1550    1992 
   Size:        520     814     216     442

  Fragments arranged by size: 

                 814     520     442     216
 
AlwNI CAG_nnn'CTG

Cuts at:      0     796     972    1248    1992 
   Size:        796     176     276     744

  Fragments arranged by size: 

                 796     744     276     176
 
ApoI r'AATT_y

Cuts at:      0    1254    1378    1992 
   Size:       1254     124     614

  Fragments arranged by size: 

                1254     614     124
 
AseI AT'TA_AT

Cuts at:      0    1826    1882    1992 
   Size:       1826      56     110

  Fragments arranged by size: 

                1826     110      56
 
AvaI C'yCGr_G

Cuts at:      0    1568    1992 
   Size:       1568     424
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1640    1673    1992 
   Size:       1640      33     319

  Fragments arranged by size: 

                1640     319      33
 
BamHI G'GATC_C

Cuts at:      0    1860    1992 
   Size:       1860     132
 
BanI G'GyrC_C

Cuts at:      0      91    1992 
   Size:         91    1901
 
BanII G_rGCy'C

Cuts at:      0     150     596    1992 
   Size:        150     446    1396

  Fragments arranged by size: 

                1396     446     150
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     484    1013    1992 
   Size:        484     529     979

  Fragments arranged by size: 

                 979     529     484
 
BclI T'GATC_A

Cuts at:      0    1386    1992 
   Size:       1386     606
 
BglII A'GATC_T

Cuts at:      0    1127    1992 
   Size:       1127     865
 
Bme1580I G_kGCm'C

Cuts at:      0     552    1992 
   Size:        552    1440
 
BmtI G_CTAG'C

Cuts at:      0     410    1992 
   Size:        410    1582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     773     974    1048    1104    1778    1992 
   Size:        106     667     201      74      56     674     214

  Fragments arranged by size: 

                 674     667     214     201     106      74      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1637    1992 
   Size:       1637     355
 
BsaBI GATnn'nnATC

Cuts at:      0    1955    1992 
   Size:       1955      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     514     544    1102    1132    1392    1422    1992 
   Size:        514      30     558      30     260      30     570

  Fragments arranged by size: 

                 570     558     514     260      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     519    1336    1992 
   Size:         43     476     817     656

  Fragments arranged by size: 

                 817     656     476      43
 
BseYI C'CCAG_C

Cuts at:      0     749    1237    1992 
   Size:        749     488     755

  Fragments arranged by size: 

                 755     749     488
 
BsmI GAATG_Cn'

Cuts at:      0     704    1992 
   Size:        704    1288
 
Bsp1286I G_dGCh'C

Cuts at:      0     150     552     596    1992 
   Size:        150     402      44    1396

  Fragments arranged by size: 

                1396     402     150      44
 
BsrBI CCG'CTC

Cuts at:      0    1180    1992 
   Size:       1180     812
 
BsrDI GCAATG_nn'

Cuts at:      0     134     417    1992 
   Size:        134     283    1575

  Fragments arranged by size: 

                1575     283     134
 
BsrFI r'CCGG_y

Cuts at:      0    1010    1992 
   Size:       1010     982
 
BstEII G'GTnAC_C

Cuts at:      0     844     876    1614    1992 
   Size:        844      32     738     378

  Fragments arranged by size: 

                 844     738     378      32
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     276     663     841    1992 
   Size:        276     387     178    1151

  Fragments arranged by size: 

                1151     387     276     178
 
BstYI r'GATC_y

Cuts at:      0     363     390    1127    1641    1860    1992 
   Size:        363      27     737     514     219     132

  Fragments arranged by size: 

                 737     514     363     219     132      27
 
Bsu36I CC'TnA_GG

Cuts at:      0    1148    1992 
   Size:       1148     844
 
BtgI C'CryG_G

Cuts at:      0       8     503     612     947     995    1992 
   Size:          8     495     109     335      48     997

  Fragments arranged by size: 

                 997     495     335     109      48       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1561    1992 
   Size:       1561     431
 
BtsI GCAGTG_nn'

Cuts at:      0    1196    1992 
   Size:       1196     796
 
DraI TTT'AAA

Cuts at:      0     386    1992 
   Size:        386    1606
 
EaeI y'GGCC_r

Cuts at:      0     167     500     581     803     992    1992 
   Size:        167     333      81     222     189    1000

  Fragments arranged by size: 

                1000     333     222     189     167      81
 
EarI CTCTTCn'nnn_

Cuts at:      0    1085    1992 
   Size:       1085     907
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     528     745    1992 
   Size:        528     217    1247

  Fragments arranged by size: 

                1247     528     217
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     520     773     974    1048    1104    1334    1550 
   Size:        106     414     253     201      74      56     230     216

Cuts at:   1550    1778    1992 
   Size:        228     214

  Fragments arranged by size: 

                 414     253     230     228     216     214     201     106
                  74      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25     891    1992 
   Size:         25     866    1101

  Fragments arranged by size: 

                1101     866      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     969    1992 
   Size:        969    1023
 
EcoRI G'AATT_C

Cuts at:      0    1378    1992 
   Size:       1378     614
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     778    1480    1992 
   Size:        778     702     512

  Fragments arranged by size: 

                 778     702     512
 
HaeII r_GCGC'y

Cuts at:      0    1017    1992 
   Size:       1017     975
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     947     967     979     999    1423    1455    1992 
   Size:        947      20      12      20     424      32     537

  Fragments arranged by size: 

                 947     537     424      32      20      20      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     468    1992 
   Size:        468    1524
 
MscI TGG'CCA

Cuts at:      0     169     583    1992 
   Size:        169     414    1409

  Fragments arranged by size: 

                1409     414     169
 
MspA1I CmG'CkG

Cuts at:      0     117     505    1992 
   Size:        117     388    1487

  Fragments arranged by size: 

                1487     388     117
 
NcoI C'CATG_G

Cuts at:      0     612    1992 
   Size:        612    1380
 
NdeI CA'TA_TG

Cuts at:      0     201    1992 
   Size:        201    1791
 
NheI G'CTAG_C

Cuts at:      0     406    1992 
   Size:        406    1586
 
NruI TCG'CGA

Cuts at:      0    1159    1992 
   Size:       1159     833
 
PasI CC'CwG_GG

Cuts at:      0     938    1992 
   Size:        938    1054
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     887     972    1611    1992 
   Size:        887      85     639     381

  Fragments arranged by size: 

                 887     639     381      85
 
PfoI T'CCnGG_A

Cuts at:      0     965    1992 
   Size:        965    1027
 
PpuMI rG'GwC_Cy

Cuts at:      0     969    1992 
   Size:        969    1023
 
PshAI GACnn'nnGTC

Cuts at:      0     484    1992 
   Size:        484    1508
 
PstI C_TGCA'G

Cuts at:      0     566    1992 
   Size:        566    1426
 
PvuII CAG'CTG

Cuts at:      0     117    1992 
   Size:        117    1875
 
SacII CC_GC'GG

Cuts at:      0     506    1992 
   Size:        506    1486
 
ScaI AGT'ACT

Cuts at:      0     541    1992 
   Size:        541    1451
 
SfcI C'TryA_G

Cuts at:      0     562    1373    1992 
   Size:        562     811     619

  Fragments arranged by size: 

                 811     619     562
 
SmlI C'TyrA_G

Cuts at:      0    1652    1992 
   Size:       1652     340
 
StyI C'CwwG_G

Cuts at:      0     612     912    1992 
   Size:        612     300    1080

  Fragments arranged by size: 

                1080     612     300
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     496     925    1153    1992 
   Size:        496     429     228     839

  Fragments arranged by size: 

                 839     496     429     228
 
TatI w'GTAC_w

Cuts at:      0     539     566    1774    1920    1992 
   Size:        539      27    1208     146      72

  Fragments arranged by size: 

                1208     539     146      72      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     250     282     482     514     546    1992 
   Size:        250      32     200      32      32    1446

  Fragments arranged by size: 

                1446     250     200      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     945    1992 
   Size:        945    1047
 
XmnI GAAnn'nnTTC

Cuts at:      0    1442    1992 
   Size:       1442     550
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI     BclI    BglII Bme1580I     BmtI     BpmI
    BpuEI    BsaBI    BsaXI    BseRI    BseYI     BsmI Bsp1286I    BsrBI
    BsrDI    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I     MmeI     MscI   MspA1I     NcoI
     NdeI     NheI     NruI     PasI    PflMI     PfoI    PpuMI    PshAI
     PstI    PvuII    SacII     ScaI     SfcI     SmlI     StyI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I     ClaI    CspCI
   DraIII     DrdI     EagI  EcoICRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI   NgoMIV     NotI     NsiI     NspI     PacI     PciI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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