Restriction map for pF1KB6193
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbvCI       BciVI       
BfrI        BglI        BlnI        BsaAI       BsaBI       
BsaI        BseAI       BsiEI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrGI       
BssHII      BstBI       BstEII      BstPI       BstUI       
Bsu36I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacII       ScaI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcyI         1961  GGCGCC               G[GA]^CG[CT]C
BanI         1961  GGCGCC               G^G[CT][GA]CC
BbeI         1961  GGCGCC               GGCGC^C
BcgI          293  GCAGCGGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1793  TGATCA               T^GATCA
BfaI          184  CTAG                 C^TAG
BlpI          867  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      867  GCTGAGC              GC^T[ACGT]AGC
BsaHI        1961  GGCGCC               G[GA]^CG[CT]C
BsaWI        1092  ACCGGA               [AT]^CCGG[AT]
BsgI          123  GTGCAG               GTGCAG(16/14)
BsiWI        1820  CGTACG               C^GTACG
BsmI          441  GAATGC               GAATGC(1/-1)
BspHI         474  TCATGA               T^CATGA
Bst1107I     1609  GTATAC               GTA^TAC
BstAPI       1851  GCAGCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         653  CCAGTAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1609  GTATAC               GTA^TAC
CelII         867  GCTGAGC              GC^T[ACGT]AGC
DraII         197  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1420  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1824  CGGCCA               [CT]^GGCC[GA]
EcoNI         810  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      197  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I      1718  CCATGG               C^C[AT][AT]GG
FbaI         1793  TGATCA               T^GATCA
HaeII        1961  GGCGCC               [GA]GCGC^[CT]
HgaI         1950  GACGC                GACGC(5/10)
Hin1I        1961  GGCGCC               G[GA]^CG[CT]C
HincII       1084  GTCGAC               GT[CT]^[GA]AC
HindII       1084  GTCGAC               GT[CT]^[GA]AC
KasI         1961  GGCGCC               G^GCGCC
MaeI          184  CTAG                 C^TAG
NarI         1961  GGCGCC               GG^CGCC
NcoI         1718  CCATGG               C^CATGG
PflMI         696  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         197  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          474  TCATGA               T^CATGA
SalI         1084  GTCGAC               G^TCGAC
SapI         1396  GAAGAGC              GCTCTTC(1/4)
SbfI         1857  CCTGCAGG             CCTGCA^GG
SexAI        1604  ACCAGGT              A^CC[AT]GGT
SfoI         1961  GGCGCC               GGC^GCC
Sse8387I     1857  CCTGCAGG             CCTGCA^GG
StyI         1718  CCATGG               C^C[AT][AT]GG
Van91I        696  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AseI          427  ATTAAT               AT^TAAT
             1135  ATTAAT              
AsnI          427  ATTAAT               AT^TAAT
             1135  ATTAAT              
Asp700I       897  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1195  GAAGACATTC          
BglII         899  AGATCT               A^GATCT
             1727  AGATCT              
BsrBI        1894  GAGCGG               CCGCTC(-3/-3)
             1933  GAGCGG              
BsrDI         666  CATTGC               GCAATG(2/0)
             1622  CATTGC              
BsrFI        1401  GCCGGC               [GA]^CCGG[CT]
             1964  GCCGGC              
BssSI        1225  CACGAG               CACGAG(-5/-1)
              799  CTCGTG              
Cfr10I       1401  GCCGGC               [GA]^CCGG[CT]
             1964  GCCGGC              
DsaI          953  CCACGG               C^C[GA][CT]GG
             1718  CCATGG              
MaeII         785  ACGT                 A^CGT
             1819  ACGT                
NaeI         1401  GCCGGC               GCC^GGC
             1964  GCCGGC              
NgoMIV       1401  GCCGGC               G^CCGGC
             1964  GCCGGC              
PshBI         427  ATTAAT               AT^TAAT
             1135  ATTAAT              
TfiI          467  GAATC                G^A[AT]TC
              731  GAATC               
Tsp45I        304  GTGAC                ^GT[GC]AC
             1426  GTGAC               
XcmI          308  CCAGATGCCCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              548  CCAGAGTGGCTGTGG     
XmnI          897  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1195  GAAGACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EarI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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