Restriction map for pF1KB6193
 (Linear) (Six-base) MAPSORT of: KIBB6193.seq  Check: 9071  from: 1  to: 1986



KIBB6193.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:      0    1085    1592    1610    1986 
   Size:       1085     507      18     376

  Fragments arranged by size: 

                1085     507     376      18
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     392     788     832    1819    1909    1948    1986 
   Size:        352      40     396      44     987      90      39      38

  Fragments arranged by size: 

                 987     396     352      90      44      40      39      38
            
 
AlwNI CAG_nnn'CTG

Cuts at:      0      43     201    1740    1857    1986 
   Size:         43     158    1539     117     129

  Fragments arranged by size: 

                1539     158     129     117      43
 
ApoI r'AATT_y

Cuts at:      0    1280    1303    1470    1986 
   Size:       1280      23     167     516

  Fragments arranged by size: 

                1280     516     167      23
 
AseI AT'TA_AT

Cuts at:      0     428    1136    1986 
   Size:        428     708     850

  Fragments arranged by size: 

                 850     708     428
 
AvaI C'yCGr_G

Cuts at:      0     240     503     709    1986 
   Size:        240     263     206    1277

  Fragments arranged by size: 

                1277     263     240     206
 
BanI G'GyrC_C

Cuts at:      0    1961    1986 
   Size:       1961      25
 
BanII G_rGCy'C

Cuts at:      0     183     241     253     992    1986 
   Size:        183      58      12     739     994

  Fragments arranged by size: 

                 994     739     183      58      12
 
BbeI G_GCGC'C

Cuts at:      0    1965    1986 
   Size:       1965      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     776    1103    1202    1526    1986 
   Size:        776     327      99     324     460

  Fragments arranged by size: 

                 776     460     327     324      99
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     282     316    1986 
   Size:        282      34    1670

  Fragments arranged by size: 

                1670     282      34
 
BclI T'GATC_A

Cuts at:      0    1793    1986 
   Size:       1793     193
 
BglII A'GATC_T

Cuts at:      0     899    1727    1986 
   Size:        899     828     259

  Fragments arranged by size: 

                 899     828     259
 
BlpI GC'TnA_GC

Cuts at:      0     868    1986 
   Size:        868    1118
 
BmgBI CAC'GTC

Cuts at:      0     786    1986 
   Size:        786    1200
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     647    1968    1986 
   Size:        647    1321      18

  Fragments arranged by size: 

                1321     647      18
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     516    1986 
   Size:        516    1470
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     958    1761    1798    1986 
   Size:        511     447     803      37     188

  Fragments arranged by size: 

                 803     511     447     188      37
 
BsaHI Gr'CG_yC

Cuts at:      0    1962    1986 
   Size:       1962      24
 
BsaWI w'CCGG_w

Cuts at:      0    1092    1986 
   Size:       1092     894
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     253     283    1986 
   Size:        253      30    1703

  Fragments arranged by size: 

                1703     253      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151    1078    1738    1986 
   Size:        151     927     660     248

  Fragments arranged by size: 

                 927     660     248     151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144    1986 
   Size:        144    1842
 
BsiHKAI G_wGCw'C

Cuts at:      0     241     253     992    1986 
   Size:        241      12     739     994

  Fragments arranged by size: 

                 994     739     241      12
 
BsiWI C'GTAC_G

Cuts at:      0    1820    1986 
   Size:       1820     166
 
BsmI GAATG_Cn'

Cuts at:      0     447    1986 
   Size:        447    1539
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     241     253     992    1986 
   Size:        183      58      12     739     994

  Fragments arranged by size: 

                 994     739     183      58      12
 
BspHI T'CATG_A

Cuts at:      0     474    1986 
   Size:        474    1512
 
BsrBI CCG'CTC

Cuts at:      0    1896    1935    1986 
   Size:       1896      39      51

  Fragments arranged by size: 

                1896      51      39
 
BsrDI GCAATG_nn'

Cuts at:      0     665    1621    1986 
   Size:        665     956     365

  Fragments arranged by size: 

                 956     665     365
 
BsrFI r'CCGG_y

Cuts at:      0    1401    1964    1986 
   Size:       1401     563      22

  Fragments arranged by size: 

                1401     563      22
 
BssSI C'ACGA_G

Cuts at:      0     799    1225    1986 
   Size:        799     426     761

  Fragments arranged by size: 

                 799     761     426
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1857    1986 
   Size:       1857     129
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     660    1986 
   Size:        660    1326
 
BstYI r'GATC_y

Cuts at:      0     516     854     899     930    1727    1986 
   Size:        516     338      45      31     797     259

  Fragments arranged by size: 

                 797     516     338     259      45      31
 
BstZ17I GTA'TAC

Cuts at:      0    1611    1986 
   Size:       1611     375
 
BtgI C'CryG_G

Cuts at:      0     953    1718    1986 
   Size:        953     765     268

  Fragments arranged by size: 

                 953     765     268
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       6     201     432    1986 
   Size:          6     195     231    1554

  Fragments arranged by size: 

                1554     231     195       6
 
BtsI GCAGTG_nn'

Cuts at:      0    1157    1986 
   Size:       1157     829
 
DraIII CAC_nnn'GTG

Cuts at:      0    1425    1986 
   Size:       1425     561
 
EaeI y'GGCC_r

Cuts at:      0    1824    1986 
   Size:       1824     162
 
EarI CTCTTCn'nnn_

Cuts at:      0    1391    1472    1719    1986 
   Size:       1391      81     247     267

  Fragments arranged by size: 

                1391     267     247      81
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1552    1986 
   Size:       1552     434
 
EcoICRI GAG'CTC

Cuts at:      0     239     251     990    1986 
   Size:        239      12     739     996

  Fragments arranged by size: 

                 996     739     239      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     392     511     788     832     958    1761    1798 
   Size:        352      40     119     277      44     126     803      37

Cuts at:   1798    1819    1909    1948    1986 
   Size:         21      90      39      38

  Fragments arranged by size: 

                 803     352     277     126     119      90      44      40
                  39      38      37      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     814    1986 
   Size:        814    1172
 
EcoO109I rG'GnC_Cy

Cuts at:      0     198    1986 
   Size:        198    1788
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     921    1986 
   Size:        921    1065
 
HaeII r_GCGC'y

Cuts at:      0    1965    1986 
   Size:       1965      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     253     255     285     353     385     884     916 
   Size:        223      30       2      30      68      32     499      32

Cuts at:    916    1734    1766    1941    1973    1986 
   Size:        818      32     175      32      13

  Fragments arranged by size: 

                 818     499     223     175      68      32      32      32
                  32      30      30      13       2
 
HincII GTy'rAC

Cuts at:      0    1086    1986 
   Size:       1086     900
 
KasI G'GCGC_C

Cuts at:      0    1961    1986 
   Size:       1961      25
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     239    1245    1986 
   Size:        239    1006     741

  Fragments arranged by size: 

                1006     741     239
 
MspA1I CmG'CkG

Cuts at:      0      40     296     318     867    1880    1986 
   Size:         40     256      22     549    1013     106

  Fragments arranged by size: 

                1013     549     256     106      40      22
 
NaeI GCC'GGC

Cuts at:      0    1403    1966    1986 
   Size:       1403     563      20

  Fragments arranged by size: 

                1403     563      20
 
NarI GG'CG_CC

Cuts at:      0    1962    1986 
   Size:       1962      24
 
NcoI C'CATG_G

Cuts at:      0    1718    1986 
   Size:       1718     268
 
NgoMIV G'CCGG_C

Cuts at:      0    1401    1964    1986 
   Size:       1401     563      22

  Fragments arranged by size: 

                1401     563      22
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     702    1986 
   Size:        702    1284
 
PfoI T'CCnGG_A

Cuts at:      0     512     857    1986 
   Size:        512     345    1129

  Fragments arranged by size: 

                1129     512     345
 
PpuMI rG'GwC_Cy

Cuts at:      0     198    1986 
   Size:        198    1788
 
PstI C_TGCA'G

Cuts at:      0     890    1853    1862    1986 
   Size:        890     963       9     124

  Fragments arranged by size: 

                 963     890     124       9
 
PvuII CAG'CTG

Cuts at:      0      40     867    1880    1986 
   Size:         40     827    1013     106

  Fragments arranged by size: 

                1013     827     106      40
 
SacI G_AGCT'C

Cuts at:      0     241     253     992    1986 
   Size:        241      12     739     994

  Fragments arranged by size: 

                 994     739     241      12
 
SalI G'TCGA_C

Cuts at:      0    1084    1986 
   Size:       1084     902
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1391    1986 
   Size:       1391     595
 
SbfI CC_TGCA'GG

Cuts at:      0    1862    1986 
   Size:       1862     124
 
SexAI A'CCwGG_T

Cuts at:      0    1604    1986 
   Size:       1604     382
 
SfcI C'TryA_G

Cuts at:      0     886    1184    1849    1858    1875    1986 
   Size:        886     298     665       9      17     111

  Fragments arranged by size: 

                 886     665     298     111      17       9
 
SfoI GGC'GCC

Cuts at:      0    1963    1986 
   Size:       1963      23
 
StyI C'CwwG_G

Cuts at:      0    1718    1986 
   Size:       1718     268
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     932    1986 
   Size:        932    1054
 
TatI w'GTAC_w

Cuts at:      0     611    1243    1986 
   Size:        611     632     743

  Fragments arranged by size: 

                 743     632     611
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     315     555    1986 
   Size:        315     240    1431

  Fragments arranged by size: 

                1431     315     240
 
XmnI GAAnn'nnTTC

Cuts at:      0     901    1199    1986 
   Size:        901     298     787

  Fragments arranged by size: 

                 901     787     298
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI     AvaI     BanI    BanII
     BbeI     BbsI     BcgI     BclI    BglII     BlpI    BmgBI     BmrI
     BplI     BpmI    BsaHI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII     KasI     MmeI   MspA1I     NaeI     NarI
     NcoI   NgoMIV    PflMI     PfoI    PpuMI     PstI    PvuII     SacI
     SalI     SapI     SbfI    SexAI     SfcI     SfoI     StyI    TaqII
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI    BbvCI    BciVI    BfrBI     BglI Bme1580I
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BseYI    BsiEI
    BsmBI    BspEI    BspMI    BsrGI   BssHII    BstBI   BstEII   Bsu36I
     ClaI    CspCI     DraI     DrdI     EagI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     MslI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     ScaI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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