Restriction map for pF1KB6169
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     AvrII       BamHI       
BbeI        BbvCI       BcgI        BclI        BfaI        
BfrI        BglII       BlnI        BseAI       BsgI        
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstBI       BstYI       BstZ17I     ClaI        CpoI        
DraI        Eco47III    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HindIII     HpaI        KasI        
MaeI        MfeI        MflI        MluI        MroI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tsp509I     XbaI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       1052  GGTACC               G^GTACC
AccI          433  GTCGAC               GT^[AC][GT]AC
AlwNI         251  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I      1052  GGTACC               G^GTACC
AviII         509  TGCGCA               TGC^GCA
BbrPI         295  CACGTG               CAC^GTG
BciVI        1063  GGATAC               GTATCC(6/5)
BsaAI         295  CACGTG               [CT]AC^GT[GA]
BsaBI         582  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         592  ACCGGA               [AT]^CCGG[AT]
BsmBI        1073  GAGACG               CGTCTC(1/5)
BspMI        1529  GCAGGT               ACCTGC(4/8)
BssHII         80  GCGCGC               G^CGCGC
BstAPI        239  GCACCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        273  GGTGACC              G^GT[ACGT]ACC
BstPI         273  GGTGACC              G^GT[ACGT]ACC
BstXI         170  CCAGAGGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI          208  GACGCGGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1308  CCTCCACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       273  GGTGACC              G^GT[ACGT]ACC
FspI          509  TGCGCA               TGC^GCA
KpnI         1052  GGTACC               GGTAC^C
KspI         1579  CCGCGG               CCGC^GG
MamI          582  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          267  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1031  CCATGG               C^CATGG
NspI         1571  ACATGC               [GA]CATG^[CT]
PaeR7I       1620  CTCGAG               C^TCGAG
PflMI        1403  CCAGGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         295  CACGTG               CAC^GTG
PmlI          295  CACGTG               CAC^GTG
SacII        1579  CCGCGG               CCGC^GG
SalI          433  GTCGAC               G^TCGAC
SapI          780  GCTCTTC              GCTCTTC(1/4)
SfcI         1170  CTACAG               C^T[GA][CT]AG
Van91I       1403  CCAGGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1268  CCAGTGGCCGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1620  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI         1483  GACGCC               G[GA]^CG[CT]C
             1890  GGCGTC              
AhdI         1660  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1690  GACCCCAGGTC         
Alw44I         58  GTGCAC               G^TGCAC
              246  GTGCAC              
AlwI          303  GATCC                GGATC(4/5)
             1362  GATCC               
ApaLI          58  GTGCAC               G^TGCAC
              246  GTGCAC              
AspEI        1660  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1690  GACCCCAGGTC         
AspI          208  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              276  GACCGTGTC           
BbsI          772  GAAGAC               GAAGAC(2/6)
              217  GTCTTC              
BpuAI         772  GAAGAC               GAAGAC(2/6)
              217  GTCTTC              
BsaHI        1483  GACGCC               G[GA]^CG[CT]C
             1890  GGCGTC              
BsrBI        1473  CCGCTC               CCGCTC(-3/-3)
             1282  GAGCGG              
BsrFI         157  GCCGGC               [GA]^CCGG[CT]
              931  GCCGGC              
BssSI        1312  CACGAG               CACGAG(-5/-1)
             1539  CACGAG              
Cfr10I        157  GCCGGC               [GA]^CCGG[CT]
              931  GCCGGC              
DraIII        240  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              295  CACGTGGTG           
EagI          137  CGGCCG               C^GGCCG
              191  CGGCCG              
Eam1105I     1660  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1690  GACCCCAGGTC         
EclXI         137  CGGCCG               C^GGCCG
              191  CGGCCG              
Eco52I        137  CGGCCG               C^GGCCG
              191  CGGCCG              
HaeII         147  GGCGCT               [GA]GCGC^[CT]
             1676  AGCGCC              
Hin1I        1483  GACGCC               G[GA]^CG[CT]C
             1890  GGCGTC              
HincII        433  GTCGAC               GT[CT]^[GA]AC
             1078  GTTGAC              
HindII        433  GTCGAC               GT[CT]^[GA]AC
             1078  GTTGAC              
NaeI          157  GCCGGC               GCC^GGC
              931  GCCGGC              
NgoMIV        157  GCCGGC               G^CCGGC
              931  GCCGGC              
PflFI         208  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              276  GACCGTGTC           
PvuII         454  CAGCTG               CAG^CTG
             1094  CAGCTG              
SacI          703  GAGCTC               GAGCT^C
              739  GAGCTC              
SexAI         241  ACCTGGT              A^CC[AT]GGT
              747  ACCTGGT             
SmlI         1620  CTCGAG               C^T[CT][GA]AG
             1909  CTCAAG              
StuI          692  AGGCCT               AGG^CCT
             1841  AGGCCT              
TfiI         1026  GATTC                G^A[AT]TC
             1176  GATTC               
Tth111I       208  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              276  GACCGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AocI        
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaI        
BsaJI       BseRI       BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmaI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XmaCI       XmaI        
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