Restriction map for pF1KB6162
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AvrII       
BbvCI       BciVI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaWI       
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BsrGI       BssHII      BstBI       BstEII      BstPI       
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NheI        NotI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuII       RsrII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccI         1092  GTATAC               GT^[AC][GT]AC
AcsI          214  GAATTC               [GA]^AATT[CT]
AflIII        266  ACATGT               A^C[GA][CT]GT
AlwNI        1669  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1878  CCTCAGG              CC^T[ACGT]AGG
ApoI          214  GAATTC               [GA]^AATT[CT]
Asp700I       210  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          853  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1694  TGCGCA               TGC^GCA
BamHI        1301  GGATCC               G^GATCC
BanII        1486  GAGCTC               G[GA]GC[CT]^C
BbeI          865  GGCGCC               GGCGC^C
BbrPI          18  CACGTG               CAC^GTG
BcgI         1154  GCAAGATTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1928  AGATCT               A^GATCT
BsaAI          18  CACGTG               [CT]AC^GT[GA]
BsaI          616  GAGACC               GGTCTC(1/5)
BsiEI         252  CGATCG               CG[GA][CT]^CG
BsmBI        1515  CGTCTC               CGTCTC(1/5)
BsmI          426  GAATGC               GAATGC(1/-1)
BspHI         788  TCATGA               T^CATGA
BspLU11I      266  ACATGT               A^CATGT
BspMI         644  GCAGGT               ACCTGC(4/8)
BssSI         540  CACGAG               CACGAG(-5/-1)
Bst1107I     1092  GTATAC               GTA^TAC
BstXI         605  CCAATGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1092  GTATAC               GTA^TAC
Bsu36I       1878  CCTCAGG              CC^T[ACGT]AGG
DraIII       1178  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1878  CCTCAGG              CC^T[ACGT]AGG
EcoNI         438  CCTGGCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         214  GAATTC               G^AATTC
EcoT14I      1001  CCATGG               C^C[AT][AT]GG
FspI         1694  TGCGCA               TGC^GCA
HgaI         1187  GCGTC                GACGC(5/10)
KasI          865  GGCGCC               G^GCGCC
KspI          630  CCGCGG               CCGC^GG
NaeI         1867  GCCGGC               GCC^GGC
NarI          865  GGCGCC               GG^CGCC
NcoI         1001  CCATGG               C^CATGG
NgoMIV       1867  GCCGGC               G^CCGGC
NruI          255  TCGCGA               TCG^CGA
PaeR7I        107  CTCGAG               C^TCGAG
PflFI         853  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1560  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          232  GACTC                GAGTC(4/5)
PmaCI          18  CACGTG               CAC^GTG
PmlI           18  CACGTG               CAC^GTG
PstI         1339  CTGCAG               CTGCA^G
PvuI          252  CGATCG               CGAT^CG
RcaI          788  TCATGA               T^CATGA
SacI         1486  GAGCTC               GAGCT^C
SacII         630  CCGCGG               CCGC^GG
SapI          346  GAAGAGC              GCTCTTC(1/4)
ScaI          242  AGTACT               AGT^ACT
SfcI         1339  CTGCAG               C^T[GA][CT]AG
SfoI          865  GGCGCC               GGC^GCC
SphI         1664  GCATGC               GCATG^C
StyI         1001  CCATGG               C^C[AT][AT]GG
Tsp509I       215  AATT                 ^AATT
Tth111I       853  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1560  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1452  TCTAGA               T^CTAGA
XhoI          107  CTCGAG               C^TCGAG
XmnI          210  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I       1321  GGTACC               G^GTACC
             1897  GGTACC              
AcyI          865  GGCGCC               G[GA]^CG[CT]C
             1186  GGCGTC              
AlwI         1302  GATCC                GGATC(4/5)
             1301  GGATC               
Asp718I      1321  GGTACC               G^GTACC
             1897  GGTACC              
BfaI          379  CTAG                 C^TAG
             1453  CTAG                
BsaHI         865  GGCGCC               G[GA]^CG[CT]C
             1186  GGCGTC              
BsgI          638  CTGCAC               GTGCAG(16/14)
             1444  CTGCAC              
BsrBI        1719  CCGCTC               CCGCTC(-3/-3)
              138  GAGCGG              
BsrFI         574  ACCGGC               [GA]^CCGG[CT]
             1867  GCCGGC              
BsrI         1213  CCAGT                ACTGG(1/-1)
             1564  CCAGT               
BstAPI         72  GCAACCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1281  GCAGAAGGTGC         
BstYI        1301  GGATCC               [GA]^GATC[CT]
             1928  AGATCT              
Cfr10I        574  ACCGGC               [GA]^CCGG[CT]
             1867  GCCGGC              
FauI          519  GCGGG                CCCGC(4/6)
             1257  GCGGG               
Hin1I         865  GGCGCC               G[GA]^CG[CT]C
             1186  GGCGTC              
KpnI         1321  GGTACC               GGTAC^C
             1897  GGTACC              
MaeI          379  CTAG                 C^TAG
             1453  CTAG                
MflI         1301  GGATCC               [GA]^GATC[CT]
             1928  AGATCT              
PpuMI         646  AGGTCCT              [GA]G^G[AT]CC[CT]
             1569  GGGACCC             
TfiI          531  GAATC                G^A[AT]TC
              939  GATTC               
XhoII        1301  GGATCC               [GA]^GATC[CT]
             1928  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaI        AvaII       BalI        BanI        BbsI        
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrDI       
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StuI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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