Restriction map for pF1KB6162
 (Linear) (Six-base) MAPSORT of: KIBB6162.seq  Check: 8998  from: 1  to: 1941



KIBB6162.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:      0    1093    1941 
   Size:       1093     848
 
Acc65I G'GTAC_C

Cuts at:      0    1321    1897    1941 
   Size:       1321     576      44

  Fragments arranged by size: 

                1321     576      44
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1479    1941 
   Size:       1479     462
 
AflIII A'CryG_T

Cuts at:      0     266    1941 
   Size:        266    1675
 
AleI CACnn'nnGTG

Cuts at:      0    1549    1941 
   Size:       1549     392
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     517    1941 
   Size:        517    1424
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     104     136    1941 
   Size:        104      32    1805

  Fragments arranged by size: 

                1805     104      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1674    1941 
   Size:       1674     267
 
ApoI r'AATT_y

Cuts at:      0     214    1941 
   Size:        214    1727
 
AvaI C'yCGr_G

Cuts at:      0      35     107     373    1915    1941 
   Size:         35      72     266    1542      26

  Fragments arranged by size: 

                1542     266      72      35      26
 
BamHI G'GATC_C

Cuts at:      0    1301    1941 
   Size:       1301     640
 
BanI G'GyrC_C

Cuts at:      0      87     840     865    1134    1321    1897    1941 
   Size:         87     753      25     269     187     576      44

  Fragments arranged by size: 

                 753     576     269     187      87      44      25
 
BanII G_rGCy'C

Cuts at:      0    1490    1941 
   Size:       1490     451
 
BbeI G_GCGC'C

Cuts at:      0     869    1941 
   Size:        869    1072
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1465    1547    1580    1941 
   Size:       1465      82      33     361

  Fragments arranged by size: 

                1465     361      82      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1143    1177    1941 
   Size:       1143      34     764

  Fragments arranged by size: 

                1143     764      34
 
BglII A'GATC_T

Cuts at:      0    1928    1941 
   Size:       1928      13
 
Bme1580I G_kGCm'C

Cuts at:      0     845    1051    1139    1941 
   Size:        845     206      88     802

  Fragments arranged by size: 

                 845     802     206      88
 
BmgBI CAC'GTC

Cuts at:      0     804    1941 
   Size:        804    1137
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     900    1707    1872    1941 
   Size:        900     807     165      69

  Fragments arranged by size: 

                 900     807     165      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     636     940     954    1384    1941 
   Size:        636     304      14     430     557

  Fragments arranged by size: 

                 636     557     430     304      14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     610    1941 
   Size:        610    1331
 
BsaAI yAC'GTr

Cuts at:      0      20    1941 
   Size:         20    1921
 
BsaHI Gr'CG_yC

Cuts at:      0     866    1187    1941 
   Size:        866     321     754

  Fragments arranged by size: 

                 866     754     321
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     104     134    1118    1148    1941 
   Size:        104      30     984      30     793

  Fragments arranged by size: 

                 984     793     104      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     125     466     976    1941 
   Size:        125     341     510     965

  Fragments arranged by size: 

                 965     510     341     125
 
BseYI C'CCAG_C

Cuts at:      0      68     195     477     760     975    1138    1559    1941 
   Size:         68     127     282     283     215     163     421     382

  Fragments arranged by size: 

                 421     382     283     282     215     163     127      68
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    1429    1941 
   Size:        623     806     512

  Fragments arranged by size: 

                 806     623     512
 
BsiEI CG_ry'CG

Cuts at:      0     255    1941 
   Size:        255    1686
 
BsiHKAI G_wGCw'C

Cuts at:      0     547    1278    1490    1941 
   Size:        547     731     212     451

  Fragments arranged by size: 

                 731     547     451     212
 
BsmI GAATG_Cn'

Cuts at:      0     432    1941 
   Size:        432    1509
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1521    1941 
   Size:       1521     420
 
Bsp1286I G_dGCh'C

Cuts at:      0     547     845    1051    1139    1278    1490    1941 
   Size:        547     298     206      88     139     212     451

  Fragments arranged by size: 

                 547     451     298     212     206     139      88
 
BspHI T'CATG_A

Cuts at:      0     788    1941 
   Size:        788    1153
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     635    1941 
   Size:        635    1306
 
BsrBI CCG'CTC

Cuts at:      0     140    1721    1941 
   Size:        140    1581     220

  Fragments arranged by size: 

                1581     220     140
 
BsrDI GCAATG_nn'

Cuts at:      0     309    1145    1196    1941 
   Size:        309     836      51     745

  Fragments arranged by size: 

                 836     745     309      51
 
BsrFI r'CCGG_y

Cuts at:      0     574    1867    1941 
   Size:        574    1293      74

  Fragments arranged by size: 

                1293     574      74
 
BssSI C'ACGA_G

Cuts at:      0     540    1941 
   Size:        540    1401
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      78    1287    1941 
   Size:         78    1209     654

  Fragments arranged by size: 

                1209     654      78
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     612    1941 
   Size:        612    1329
 
BstYI r'GATC_y

Cuts at:      0    1301    1928    1941 
   Size:       1301     627      13

  Fragments arranged by size: 

                1301     627      13
 
BstZ17I GTA'TAC

Cuts at:      0    1094    1941 
   Size:       1094     847
 
Bsu36I CC'TnA_GG

Cuts at:      0    1879    1941 
   Size:       1879      62
 
BtgI C'CryG_G

Cuts at:      0     316     630    1001    1656    1941 
   Size:        316     314     371     655     285

  Fragments arranged by size: 

                 655     371     316     314     285
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     570     585    1941 
   Size:        570      15    1356

  Fragments arranged by size: 

                1356     570      15
 
BtsI GCAGTG_nn'

Cuts at:      0      53    1383    1640    1941 
   Size:         53    1330     257     301

  Fragments arranged by size: 

                1330     301     257      53
 
DraIII CAC_nnn'GTG

Cuts at:      0    1183    1941 
   Size:       1183     758
 
EaeI y'GGCC_r

Cuts at:      0      14     809    1004    1527    1941 
   Size:         14     795     195     523     414

  Fragments arranged by size: 

                 795     523     414     195      14
 
EarI CTCTTCn'nnn_

Cuts at:      0     341     395    1915    1941 
   Size:        341      54    1520      26

  Fragments arranged by size: 

                1520     341      54      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     262    1615    1941 
   Size:        262    1353     326

  Fragments arranged by size: 

                1353     326     262
 
EcoICRI GAG'CTC

Cuts at:      0    1488    1941 
   Size:       1488     453
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     900    1479    1707    1872    1941 
   Size:        900     579     228     165      69

  Fragments arranged by size: 

                 900     579     228     165      69
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     442    1941 
   Size:        442    1499
 
EcoO109I rG'GnC_Cy

Cuts at:      0     647    1359    1570    1941 
   Size:        647     712     211     371

  Fragments arranged by size: 

                 712     647     371     211
 
EcoRI G'AATT_C

Cuts at:      0     214    1941 
   Size:        214    1727
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     234    1080    1308    1941 
   Size:        234     846     228     633

  Fragments arranged by size: 

                 846     633     234     228
 
FspI TGC'GCA

Cuts at:      0    1696    1941 
   Size:       1696     245
 
HaeII r_GCGC'y

Cuts at:      0     150     438     869    1272    1617    1941 
   Size:        150     288     431     403     345     324

  Fragments arranged by size: 

                 431     403     345     324     288     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249     844     876    1941 
   Size:        217      32     595      32    1065

  Fragments arranged by size: 

                1065     595     217      32      32
 
KasI G'GCGC_C

Cuts at:      0     865    1941 
   Size:        865    1076
 
KpnI G_GTAC'C

Cuts at:      0    1325    1901    1941 
   Size:       1325     576      40

  Fragments arranged by size: 

                1325     576      40
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1250    1941 
   Size:       1250     691
 
MscI TGG'CCA

Cuts at:      0      16     811    1006    1941 
   Size:         16     795     195     935

  Fragments arranged by size: 

                 935     795     195      16
 
MslI CAynn'nnrTG

Cuts at:      0     589     802    1027    1549    1941 
   Size:        589     213     225     522     392

  Fragments arranged by size: 

                 589     522     392     225     213
 
MspA1I CmG'CkG

Cuts at:      0     510     632    1257    1373    1941 
   Size:        510     122     625     116     568

  Fragments arranged by size: 

                 625     568     510     122     116
 
NaeI GCC'GGC

Cuts at:      0    1869    1941 
   Size:       1869      72
 
NarI GG'CG_CC

Cuts at:      0     866    1941 
   Size:        866    1075
 
NcoI C'CATG_G

Cuts at:      0    1001    1941 
   Size:       1001     940
 
NgoMIV G'CCGG_C

Cuts at:      0    1867    1941 
   Size:       1867      74
 
NruI TCG'CGA

Cuts at:      0     257    1941 
   Size:        257    1684
 
NspI r_CATG'y

Cuts at:      0     270    1668    1683    1941 
   Size:        270    1398      15     258

  Fragments arranged by size: 

                1398     270     258      15
 
PasI CC'CwG_GG

Cuts at:      0    1506    1941 
   Size:       1506     435
 
PciI A'CATG_T

Cuts at:      0     266    1941 
   Size:        266    1675
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1566    1941 
   Size:       1566     375
 
PmlI CAC'GTG

Cuts at:      0      20    1941 
   Size:         20    1921
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136    1941 
   Size:        104      32    1805

  Fragments arranged by size: 

                1805     104      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     647    1570    1941 
   Size:        647     923     371

  Fragments arranged by size: 

                 923     647     371
 
PstI C_TGCA'G

Cuts at:      0    1343    1941 
   Size:       1343     598
 
PvuI CG_AT'CG

Cuts at:      0     255    1941 
   Size:        255    1686
 
SacI G_AGCT'C

Cuts at:      0    1490    1941 
   Size:       1490     451
 
SacII CC_GC'GG

Cuts at:      0     633    1941 
   Size:        633    1308
 
SanDI GG'GwC_CC

Cuts at:      0    1570    1941 
   Size:       1570     371
 
SapI GCTCTTCn'nnn_

Cuts at:      0     341    1941 
   Size:        341    1600
 
ScaI AGT'ACT

Cuts at:      0     244    1941 
   Size:        244    1697
 
SfcI C'TryA_G

Cuts at:      0    1339    1941 
   Size:       1339     602
 
SfoI GGC'GCC

Cuts at:      0     867    1941 
   Size:        867    1074
 
SmlI C'TyrA_G

Cuts at:      0     107     651     933     955    1363    1941 
   Size:        107     544     282      22     408     578

  Fragments arranged by size: 

                 578     544     408     282     107      22
 
SphI G_CATG'C

Cuts at:      0    1668    1941 
   Size:       1668     273
 
StuI AGG'CCT

Cuts at:      0      11     337     688    1941 
   Size:         11     326     351    1253

  Fragments arranged by size: 

                1253     351     326      11
 
StyI C'CwwG_G

Cuts at:      0    1001    1941 
   Size:       1001     940
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     428     585    1941 
   Size:        428     157    1356

  Fragments arranged by size: 

                1356     428     157
 
TatI w'GTAC_w

Cuts at:      0     242     714    1343    1941 
   Size:        242     472     629     598

  Fragments arranged by size: 

                 629     598     472     242
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1118    1150    1941 
   Size:       1118      32     791

  Fragments arranged by size: 

                1118     791      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     856    1941 
   Size:        856    1085
 
XbaI T'CTAG_A

Cuts at:      0    1452    1941 
   Size:       1452     489
 
XhoI C'TCGA_G

Cuts at:      0     107    1941 
   Size:        107    1834
 
XmnI GAAnn'nnTTC

Cuts at:      0     214    1941 
   Size:        214    1727
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BglII Bme1580I    BmgBI     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI     NspI
     PasI     PciI    PflMI     PmlI     PpiI    PpuMI     PstI     PvuI
     SacI    SacII    SanDI     SapI     ScaI     SfcI     SfoI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI     BmrI     BmtI     BplI   Bpu10I
    BsaBI    BsaWI    BsiWI    BspEI    BsrGI   BssHII    BstBI   BstEII
     ClaI    CspCI     DraI     DrdI     EagI    EcoRV     FseI    FspAI
   HincII  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI
     NsiI     PacI     PfoI     PmeI    PshAI     PsiI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SalI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XcmI
     XmaI     ZraI
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