Restriction map for pF1KB0479
 (Linear) (Six-base) MAPSORT of: KIBB0479.seq  Check: 3266  from: 1  to: 1554



KIBB0479.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     212    1554 
   Size:        212    1342
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     666     946    1554 
   Size:        541     125     280     608

  Fragments arranged by size: 

                 608     541     280     125
 
AleI CACnn'nnGTG

Cuts at:      0     697    1554 
   Size:        697     857
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     385     754    1237    1554 
   Size:        385     369     483     317

  Fragments arranged by size: 

                 483     385     369     317
 
AlwNI CAG_nnn'CTG

Cuts at:      0     204     388     474     924    1410    1533    1554 
   Size:        204     184      86     450     486     123      21

  Fragments arranged by size: 

                 486     450     204     184     123      86      21
 
ApaI G_GGCC'C

Cuts at:      0     642     881    1554 
   Size:        642     239     673

  Fragments arranged by size: 

                 673     642     239
 
AvaI C'yCGr_G

Cuts at:      0     314     650    1501    1517    1554 
   Size:        314     336     851      16      37

  Fragments arranged by size: 

                 851     336     314      37      16
 
BanI G'GyrC_C

Cuts at:      0     708     768     866    1513    1554 
   Size:        708      60      98     647      41

  Fragments arranged by size: 

                 708     647      98      60      41
 
BanII G_rGCy'C

Cuts at:      0     642     881    1235    1554 
   Size:        642     239     354     319

  Fragments arranged by size: 

                 642     354     319     239
 
BbeI G_GCGC'C

Cuts at:      0     870    1517    1554 
   Size:        870     647      37

  Fragments arranged by size: 

                 870     647      37
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      83     117    1554 
   Size:         83      34    1437

  Fragments arranged by size: 

                1437      83      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     348    1554 
   Size:        348    1206
 
BglI GCCn_nnn'nGGC

Cuts at:      0     627    1554 
   Size:        627     927
 
Bme1580I G_kGCm'C

Cuts at:      0       7     642     840     881    1018    1554 
   Size:          7     635     198      41     137     536

  Fragments arranged by size: 

                 635     536     198     137      41       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      27      52    1554 
   Size:         27      25    1502

  Fragments arranged by size: 

                1502      27      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     724    1393    1554 
   Size:        334     390     669     161

  Fragments arranged by size: 

                 669     390     334     161
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     245    1554 
   Size:        177      68    1309

  Fragments arranged by size: 

                1309     177      68
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     195    1120    1554 
   Size:        195     925     434

  Fragments arranged by size: 

                 925     434     195
 
BsaAI yAC'GTr

Cuts at:      0     935    1554 
   Size:        935     619
 
BsaHI Gr'CG_yC

Cuts at:      0     446     867    1514    1554 
   Size:        446     421     647      40

  Fragments arranged by size: 

                 647     446     421      40
 
BsaWI w'CCGG_w

Cuts at:      0    1037    1331    1554 
   Size:       1037     294     223

  Fragments arranged by size: 

                1037     294     223
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     400     430    1117    1147    1435    1465    1554 
   Size:        400      30     687      30     288      30      89

  Fragments arranged by size: 

                 687     400     288      89      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1180    1399    1554 
   Size:       1180     219     155

  Fragments arranged by size: 

                1180     219     155
 
BseYI C'CCAG_C

Cuts at:      0     117     161     562    1199    1554 
   Size:        117      44     401     637     355

  Fragments arranged by size: 

                 637     401     355     117      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1396    1554 
   Size:       1396     158
 
BsiEI CG_ry'CG

Cuts at:      0     634    1554 
   Size:        634     920
 
BsiHKAI G_wGCw'C

Cuts at:      0    1235    1554 
   Size:       1235     319
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     642     840     881    1018    1235    1554 
   Size:          7     635     198      41     137     217     319

  Fragments arranged by size: 

                 635     319     217     198     137      41       7
 
BspEI T'CCGG_A

Cuts at:      0    1037    1554 
   Size:       1037     517
 
BspHI T'CATG_A

Cuts at:      0    1421    1554 
   Size:       1421     133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     212    1554 
   Size:        212    1342
 
BsrBI CCG'CTC

Cuts at:      0     411    1025    1437    1554 
   Size:        411     614     412     117

  Fragments arranged by size: 

                 614     412     411     117
 
BsrDI GCAATG_nn'

Cuts at:      0    1287    1554 
   Size:       1287     267
 
BsrFI r'CCGG_y

Cuts at:      0    1276    1554 
   Size:       1276     278
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     204    1410    1554 
   Size:        204    1206     144

  Fragments arranged by size: 

                1206     204     144
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1137    1554 
   Size:       1137     417
 
BstYI r'GATC_y

Cuts at:      0    1328    1554 
   Size:       1328     226
 
BtgI C'CryG_G

Cuts at:      0     272     330     504     858    1554 
   Size:        272      58     174     354     696

  Fragments arranged by size: 

                 696     354     272     174      58
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     437    1554 
   Size:        437    1117
 
BtsI GCAGTG_nn'

Cuts at:      0      99     214     838    1554 
   Size:         99     115     624     716

  Fragments arranged by size: 

                 716     624     115      99
 
DraIII CAC_nnn'GTG

Cuts at:      0     385    1554 
   Size:        385    1169
 
EaeI y'GGCC_r

Cuts at:      0     631     746    1364    1554 
   Size:        631     115     618     190

  Fragments arranged by size: 

                 631     618     190     115
 
EagI C'GGCC_G

Cuts at:      0     631    1554 
   Size:        631     923
 
EarI CTCTTCn'nnn_

Cuts at:      0     895    1554 
   Size:        895     659
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     268    1443    1554 
   Size:        268    1175     111

  Fragments arranged by size: 

                1175     268     111
 
EcoICRI GAG'CTC

Cuts at:      0    1233    1554 
   Size:       1233     321
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     541     666     724     946    1393    1554 
   Size:        334     207     125      58     222     447     161

  Fragments arranged by size: 

                 447     334     222     207     161     125      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     122     435     509     620     638     878    1498 
   Size:         54      68     313      74     111      18     240     620

Cuts at:   1498    1530    1554 
   Size:         32      24

  Fragments arranged by size: 

                 620     313     240     111      74      68      54      32
                  24      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1035    1554 
   Size:       1035     519
 
HaeII r_GCGC'y

Cuts at:      0     870    1186    1517    1554 
   Size:        870     316     331      37

  Fragments arranged by size: 

                 870     331     316      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     400     432     436     468    1156    1188    1435    1467 
   Size:        400      32       4      32     688      32     247      32

Cuts at:   1467    1554 
   Size:         87

  Fragments arranged by size: 

                 688     400     247      87      32      32      32      32
                   4
 
KasI G'GCGC_C

Cuts at:      0     866    1513    1554 
   Size:        866     647      41

  Fragments arranged by size: 

                 866     647      41
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     422    1554 
   Size:         48     374    1132

  Fragments arranged by size: 

                1132     374      48
 
MslI CAynn'nnrTG

Cuts at:      0     697    1554 
   Size:        697     857
 
MspA1I CmG'CkG

Cuts at:      0     332     744     765     981    1245    1363    1554 
   Size:        332     412      21     216     264     118     191

  Fragments arranged by size: 

                 412     332     264     216     191     118      21
 
NarI GG'CG_CC

Cuts at:      0     867    1514    1554 
   Size:        867     647      40

  Fragments arranged by size: 

                 867     647      40
 
NcoI C'CATG_G

Cuts at:      0     272     504     858    1554 
   Size:        272     232     354     696

  Fragments arranged by size: 

                 696     354     272     232
 
NspI r_CATG'y

Cuts at:      0     497    1554 
   Size:        497    1057
 
PasI CC'CwG_GG

Cuts at:      0     468     624    1554 
   Size:        468     156     930

  Fragments arranged by size: 

                 930     468     156
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     273     474     614    1533    1554 
   Size:        273     201     140     919      21

  Fragments arranged by size: 

                 919     273     201     140      21
 
PfoI T'CCnGG_A

Cuts at:      0     350    1112    1370    1554 
   Size:        350     762     258     184

  Fragments arranged by size: 

                 762     350     258     184
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     435    1554 
   Size:         54     381    1119

  Fragments arranged by size: 

                1119     381      54
 
PshAI GACnn'nnGTC

Cuts at:      0     274    1554 
   Size:        274    1280
 
PspOMI G'GGCC_C

Cuts at:      0     638     877    1554 
   Size:        638     239     677

  Fragments arranged by size: 

                 677     638     239
 
PstI C_TGCA'G

Cuts at:      0     743     833    1406    1554 
   Size:        743      90     573     148

  Fragments arranged by size: 

                 743     573     148      90
 
PvuII CAG'CTG

Cuts at:      0     744     981    1245    1554 
   Size:        744     237     264     309

  Fragments arranged by size: 

                 744     309     264     237
 
SacI G_AGCT'C

Cuts at:      0    1235    1554 
   Size:       1235     319
 
SacII CC_GC'GG

Cuts at:      0     333    1554 
   Size:        333    1221
 
SanDI GG'GwC_CC

Cuts at:      0      54    1554 
   Size:         54    1500
 
SapI GCTCTTCn'nnn_

Cuts at:      0     895    1554 
   Size:        895     659
 
SbfI CC_TGCA'GG

Cuts at:      0    1406    1554 
   Size:       1406     148
 
SexAI A'CCwGG_T

Cuts at:      0     381    1554 
   Size:        381    1173
 
SfcI C'TryA_G

Cuts at:      0     579     739     829    1402    1554 
   Size:        579     160      90     573     152

  Fragments arranged by size: 

                 579     573     160     152      90
 
SfoI GGC'GCC

Cuts at:      0     868    1515    1554 
   Size:        868     647      39

  Fragments arranged by size: 

                 868     647      39
 
SmaI CCC'GGG

Cuts at:      0     652    1519    1554 
   Size:        652     867      35

  Fragments arranged by size: 

                 867     652      35
 
SmlI C'TyrA_G

Cuts at:      0     192     260     314    1554 
   Size:        192      68      54    1240

  Fragments arranged by size: 

                1240     192      68      54
 
StyI C'CwwG_G

Cuts at:      0     272     504     608     858    1554 
   Size:        272     232     104     250     696

  Fragments arranged by size: 

                 696     272     250     232     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     270    1554 
   Size:        270    1284
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1003    1554 
   Size:       1003     551
 
XhoI C'TCGA_G

Cuts at:      0     314    1554 
   Size:        314    1240
 
XmaI C'CCGG_G

Cuts at:      0     650    1517    1554 
   Size:        650     867      37

  Fragments arranged by size: 

                 867     650      37
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BcgI    BciVI     BglI Bme1580I     BmrI     BpmI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I     NarI
     NcoI     NspI     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI
     PstI    PvuII     SacI    SacII    SanDI     SapI     SbfI    SexAI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BsaBI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XmnI     ZraI
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