Restriction map for pF1KB7322
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# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
AviII       AvrII       BbeI        BbrPI       BglI        
BlnI        BlpI        Bpu1102I    BsaHI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     FseI        
FspI        HgaI        Hin1I       KasI        KpnI        
MluI        MroI        NaeI        NarI        NgoMIV      
NotI        NruI        NspV        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PvuI        
RsrII       SalI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SpeI        Van91I      
XmaCI       XmaI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflII        5916  CTTAAG               C^TTAAG
AspI          612  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        4629  GGATCC               G^GATCC
BbvCI        4682  CCTCAGC              CCTCAGC(-5/-2)
BcgI          688  GCAGGGCAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4622  TGATCA               T^GATCA
BfrI         5916  CTTAAG               C^TTAAG
BglII        1863  AGATCT               A^GATCT
BsaBI        5438  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI         146  CGTCTC               CGTCTC(1/5)
Bsp1407I     4315  TGTACA               T^GTACA
BspHI        2493  TCATGA               T^CATGA
BsrGI        4315  TGTACA               T^GTACA
BstAPI        862  GCAATGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoNI        1476  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        4455  GATATC               GAT^ATC
EcoT22I       865  ATGCAT               ATGCA^T
FbaI         4622  TGATCA               T^GATCA
HaeII        2502  GGCGCT               [GA]GCGC^[CT]
KspI           89  CCGCGG               CCGC^GG
MamI         5438  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4606  CAATTG               C^AATTG
MunI         4606  CAATTG               C^AATTG
NcoI         2258  CCATGG               C^CATGG
NsiI          865  ATGCAT               ATGCA^T
PflFI         612  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        6903  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI        6704  GACCGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2493  TCATGA               T^CATGA
SacII          89  CCGCGG               CCGC^GG
SapI         2342  GAAGAGC              GCTCTTC(1/4)
SbfI         6802  CCTGCAGG             CCTGCA^GG
ScaI          597  AGTACT               AGT^ACT
SnaBI        3290  TACGTA               TAC^GTA
SphI          867  GCATGC               GCATG^C
Sse8387I     6802  CCTGCAGG             CCTGCA^GG
SspBI        4315  TGTACA               T^GTACA
SwaI         2219  ATTTAAAT             ATTT^AAAT
Tth111I       612  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1086  CCATAATGAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         6597  GTCTAC               GT^[AC][GT]AC
             6661  GTAGAC              
AccII          90  CGCG                 CG^CG
             1272  CGCG                
AflIII        936  ACATGT               A^C[GA][CT]GT
             1134  ACATGT              
Alw44I       4514  GTGCAC               G^TGCAC
             6440  GTGCAC              
ApaI           81  GGGCCC               GGGCC^C
             6001  GGGCCC              
ApaLI        4514  GTGCAC               G^TGCAC
             6440  GTGCAC              
AseI         1177  ATTAAT               AT^TAAT
             4612  ATTAAT              
AsnI         1177  ATTAAT               AT^TAAT
             4612  ATTAAT              
BsaAI        3290  TACGTA               [CT]AC^GT[GA]
             6545  CACGTA              
BsmI         6858  GAATGC               GAATGC(1/-1)
             1185  GCATTC              
BspLU11I      936  ACATGT               A^CATGT
             1134  ACATGT              
BstUI          90  CGCG                 CG^CG
             1272  CGCG                
DraI         1198  TTTAAA               TTT^AAA
             2220  TTTAAA              
DraIII       5077  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5924  CACAAAGTG           
HindIII       527  AAGCTT               A^AGCTT
             3805  AAGCTT              
HpaI         2199  GTTAAC               GTT^AAC
             4359  GTTAAC              
MvnI           90  CGCG                 CG^CG
             1272  CGCG                
PaeR7I       1523  CTCGAG               C^TCGAG
             2759  CTCGAG              
PshBI        1177  ATTAAT               AT^TAAT
             4612  ATTAAT              
SacI          131  GAGCTC               GAGCT^C
             3275  GAGCTC              
SspI         2646  AATATT               AAT^ATT
             2847  AATATT              
StuI         4867  AGGCCT               AGG^CCT
             5035  AGGCCT              
XbaI         2878  TCTAGA               T^CTAGA
             5413  TCTAGA              
XhoI         1523  CTCGAG               C^TCGAG
             2759  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AciI        AclI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeI        
NdeII       NheI        NlaIII      NlaIV       NspI        
PleI        Psp1406I    PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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