Restriction map for pF1KB7322
 (Linear) (Six-base) MAPSORT of: KIBB7322.seq  Check: 8025  from: 1  to: 7056



KIBB7322.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2301    3361    7056 
   Size:       2301    1060    3695

  Fragments arranged by size: 

                3695    2301    1060
 
AccI GT'mk_AC

Cuts at:      0    6598    6662    7056 
   Size:       6598      64     394

  Fragments arranged by size: 

                6598     394      64
 
AclI AA'CG_TT

Cuts at:      0    1766    3986    6537    7056 
   Size:       1766    2220    2551     519

  Fragments arranged by size: 

                2551    2220    1766     519
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     614    1834    1901    2420    3062    3559    3617 
   Size:        205     409    1220      67     519     642     497      58

Cuts at:   3617    3848    5599    5810    7056 
   Size:        231    1751     211    1246

  Fragments arranged by size: 

                1751    1246    1220     642     519     497     409     231
                 211     205      67      58
 
AflII C'TTAA_G

Cuts at:      0    5916    7056 
   Size:       5916    1140
 
AflIII A'CryG_T

Cuts at:      0     936    1134    7056 
   Size:        936     198    5922

  Fragments arranged by size: 

                5922     936     198
 
AleI CACnn'nnGTG

Cuts at:      0    5251    7056 
   Size:       5251    1805
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2957    3389    6818    7056 
   Size:       2957     432    3429     238

  Fragments arranged by size: 

                3429    2957     432     238
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1751    1783    6522    6554    6555    6587    7056 
   Size:       1751      32    4739      32       1      32     469

  Fragments arranged by size: 

                4739    1751     469      32      32      32       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0     472     592    2866    2944    3360    3493    7056 
   Size:        472     120    2274      78     416     133    3563

  Fragments arranged by size: 

                3563    2274     472     416     133     120      78
 
ApaI G_GGCC'C

Cuts at:      0      85    6005    7056 
   Size:         85    5920    1051

  Fragments arranged by size: 

                5920    1051      85
 
ApaLI G'TGCA_C

Cuts at:      0    4514    6440    7056 
   Size:       4514    1926     616

  Fragments arranged by size: 

                4514    1926     616
 
ApoI r'AATT_y

Cuts at:      0    1195    3247    4689    5124    5817    5835    5940    6123 
   Size:       1195    2052    1442     435     693      18     105     183

Cuts at:   6123    7056 
   Size:        933

  Fragments arranged by size: 

                2052    1442    1195     933     693     435     183     105
                  18
 
AseI AT'TA_AT

Cuts at:      0    1178    4613    7056 
   Size:       1178    3435    2443

  Fragments arranged by size: 

                3435    2443    1178
 
AvaI C'yCGr_G

Cuts at:      0    1523    2759    6429    7056 
   Size:       1523    1236    3670     627

  Fragments arranged by size: 

                3670    1523    1236     627
 
BamHI G'GATC_C

Cuts at:      0    4629    7056 
   Size:       4629    2427
 
BanI G'GyrC_C

Cuts at:      0     328    1369    5082    5184    5691    6312    7017    7056 
   Size:        328    1041    3713     102     507     621     705      39

  Fragments arranged by size: 

                3713    1041     705     621     507     328     102      39
            
 
BanII G_rGCy'C

Cuts at:      0      58      85     135    2232    2971    3279    5047    6005 
   Size:         58      27      50    2097     739     308    1768     958

Cuts at:   6005    7056 
   Size:       1051

  Fragments arranged by size: 

                2097    1768    1051     958     739     308      58      50
                  27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     983    2736    3046    5263    7056 
   Size:        983    1753     310    2217    1793

  Fragments arranged by size: 

                2217    1793    1753     983     310
 
BbvCI CC'TCA_GC

Cuts at:      0    4683    7056 
   Size:       4683    2373
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     677     711    7056 
   Size:        677      34    6345

  Fragments arranged by size: 

                6345     677      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     826    1785    6435    7056 
   Size:        826     959    4650     621

  Fragments arranged by size: 

                4650     959     826     621
 
BclI T'GATC_A

Cuts at:      0    4622    7056 
   Size:       4622    2434
 
BfrBI ATG'CAT

Cuts at:      0     867    7056 
   Size:        867    6189
 
BglII A'GATC_T

Cuts at:      0    1863    7056 
   Size:       1863    5193
 
Bme1580I G_kGCm'C

Cuts at:      0      85    1212    1374    4518    5988    6005    6315    6444 
   Size:         85    1127     162    3144    1470      17     310     129

Cuts at:   6444    7020    7056 
   Size:        576      36

  Fragments arranged by size: 

                3144    1470    1127     576     310     162     129      85
                  36      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2673    4846    5701    7056 
   Size:       2673    2173     855    1355

  Fragments arranged by size: 

                2673    2173    1355     855
 
BmtI G_CTAG'C

Cuts at:      0     964    1475    3325    7056 
   Size:        964     511    1850    3731

  Fragments arranged by size: 

                3731    1850     964     511
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2892    6209    7056 
   Size:       2892    3317     847

  Fragments arranged by size: 

                3317    2892     847
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1049    3173    3962    4571    5666    6146    6857    6920 
   Size:       1049    2124     789     609    1095     480     711      63

Cuts at:   6920    7056 
   Size:        136

  Fragments arranged by size: 

                2124    1095    1049     789     711     609     480     136
                  63
 
Bpu10I CC'TnA_GC

Cuts at:      0    1218    4376    4683    5069    7056 
   Size:       1218    3158     307     386    1987

  Fragments arranged by size: 

                3158    1987    1218     386     307
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1583    1700    3186    3776    7056 
   Size:       1583     117    1486     590    3280

  Fragments arranged by size: 

                3280    1583    1486     590     117
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3106    4547    6180    7056 
   Size:       3106    1441    1633     876

  Fragments arranged by size: 

                3106    1633    1441     876
 
BsaAI yAC'GTr

Cuts at:      0    3292    6547    7056 
   Size:       3292    3255     509

  Fragments arranged by size: 

                3292    3255     509
 
BsaBI GATnn'nnATC

Cuts at:      0    5442    7056 
   Size:       5442    1614
 
BsaWI w'CCGG_w

Cuts at:      0      18     230    1066    4556    7056 
   Size:         18     212     836    3490    2500

  Fragments arranged by size: 

                3490    2500     836     212      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     923     953    1022    1052    1751    1781    1949    1979 
   Size:        923      30      69      30     699      30     168      30

Cuts at:   1979    2397    2427    2737    2767    3828    3858    5505    5535 
   Size:        418      30     310      30    1061      30    1647      30

Cuts at:   5535    5643    5673    6047    6077    6555    6585    7056 
   Size:        108      30     374      30     478      30     471

  Fragments arranged by size: 

                1647    1061     923     699     478     471     418     374
                 310     168     108      69      30      30      30      30
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     140     394    1481    2222    2576    2897    3189    3401 
   Size:        140     254    1087     741     354     321     292     212

Cuts at:   3401    3643    4490    4671    4895    6338    7056 
   Size:        242     847     181     224    1443     718

  Fragments arranged by size: 

                1443    1087     847     741     718     354     321     292
                 254     242     224     212     181     140
 
BseYI C'CCAG_C

Cuts at:      0     247    5396    5747    6487    7056 
   Size:        247    5149     351     740     569

  Fragments arranged by size: 

                5149     740     569     351     247
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     635    2036    2684    3395    5297    6398    6527 
   Size:        503     132    1401     648     711    1902    1101     129

Cuts at:   6527    7056 
   Size:        529

  Fragments arranged by size: 

                1902    1401    1101     711     648     529     503     132
                 129
 
BsiHKAI G_wGCw'C

Cuts at:      0     135    1058    2579    2780    3279    4518    6330    6444 
   Size:        135     923    1521     201     499    1239    1812     114

Cuts at:   6444    7056 
   Size:        612

  Fragments arranged by size: 

                1812    1521    1239     923     612     499     201     135
                 114
 
BsmI GAATG_Cn'

Cuts at:      0    1185    6864    7056 
   Size:       1185    5679     192

  Fragments arranged by size: 

                5679    1185     192
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     152    7056 
   Size:        152    6904
 
Bsp1286I G_dGCh'C

Cuts at:      0      58      85     135    1058    1212    1374    2232    2579 
   Size:         58      27      50     923     154     162     858     347

Cuts at:   2579    2780    2971    3279    4518    5047    5988    6005    6315 
   Size:        201     191     308    1239     529     941      17     310

Cuts at:   6315    6330    6444    7020    7056 
   Size:         15     114     576      36

  Fragments arranged by size: 

                1239     941     923     858     576     529     347     310
                 308     201     191     162     154     114      58      50
                  36      27      17      15
 
BspHI T'CATG_A

Cuts at:      0    2493    7056 
   Size:       2493    4563
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     185    2301    3361    3892    5122    5473    6198    7056 
   Size:        185    2116    1060     531    1230     351     725     858

  Fragments arranged by size: 

                2116    1230    1060     858     725     531     351     185
            
 
BsrDI GCAATG_nn'

Cuts at:      0     869    2360    6498    6749    6869    7056 
   Size:        869    1491    4138     251     120     187

  Fragments arranged by size: 

                4138    1491     869     251     187     120
 
BsrGI T'GTAC_A

Cuts at:      0    4315    7056 
   Size:       4315    2741
 
BssSI C'ACGA_G

Cuts at:      0     152    2234    2530    7056 
   Size:        152    2082     296    4526

  Fragments arranged by size: 

                4526    2082     296     152
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     868    7056 
   Size:        868    6188
 
BstYI r'GATC_y

Cuts at:      0     501     588    1863    2064    4629    4952    5584    6058 
   Size:        501      87    1275     201    2565     323     632     474

Cuts at:   6058    6129    7056 
   Size:         71     927

  Fragments arranged by size: 

                2565    1275     927     632     501     474     323     201
                  87      71
 
Bsu36I CC'TnA_GG

Cuts at:      0     490    2273    6204    7056 
   Size:        490    1783    3931     852

  Fragments arranged by size: 

                3931    1783     852     490
 
BtgI C'CryG_G

Cuts at:      0      89     563    2258    5291    6974    7056 
   Size:         89     474    1695    3033    1683      82

  Fragments arranged by size: 

                3033    1695    1683     474      89      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4130    7056 
   Size:       4130    2926
 
BtsI GCAGTG_nn'

Cuts at:      0    1304    2039    2251    2548    3005    4721    5115    5309 
   Size:       1304     735     212     297     457    1716     394     194

Cuts at:   5309    7056 
   Size:       1747

  Fragments arranged by size: 

                1747    1716    1304     735     457     394     297     212
                 194
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2591    2626    2693    2728    4949    4984    5438    5473 
   Size:       2591      35      67      35    2221      35     454      35

Cuts at:   5473    6982    7017    7056 
   Size:       1509      35      39

  Fragments arranged by size: 

                2591    2221    1509     454      67      39      35      35
                  35      35      35
 
DraI TTT'AAA

Cuts at:      0    1200    2222    7056 
   Size:       1200    1022    4834

  Fragments arranged by size: 

                4834    1200    1022
 
DraIII CAC_nnn'GTG

Cuts at:      0    5082    5929    7056 
   Size:       5082     847    1127

  Fragments arranged by size: 

                5082    1127     847
 
EaeI y'GGCC_r

Cuts at:      0    1332    2121    2526    7056 
   Size:       1332     789     405    4530

  Fragments arranged by size: 

                4530    1332     789     405
 
EarI CTCTTCn'nnn_

Cuts at:      0     350     371    1475    2318    2337    3700    4226    4467 
   Size:        350      21    1104     843      19    1363     526     241

Cuts at:   4467    4892    5618    5898    7056 
   Size:        425     726     280    1158

  Fragments arranged by size: 

                1363    1158    1104     843     726     526     425     350
                 280     241      21      19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     112     326    7056 
   Size:        112     214    6730

  Fragments arranged by size: 

                6730     214     112
 
EcoICRI GAG'CTC

Cuts at:      0     133    3277    7056 
   Size:        133    3144    3779

  Fragments arranged by size: 

                3779    3144     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     614    1049    1834    1901    2420    3062    3173 
   Size:        205     409     435     785      67     519     642     111

Cuts at:   3173    3559    3617    3848    3962    4571    5599    5666    5810 
   Size:        386      58     231     114     609    1028      67     144

Cuts at:   5810    6146    6857    6920    7056 
   Size:        336     711      63     136

  Fragments arranged by size: 

                1028     785     711     642     609     519     435     409
                 386     336     231     205     144     136     114     111
                  67      67      63      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1480    7056 
   Size:       1480    5576
 
EcoO109I rG'GnC_Cy

Cuts at:      0      82    6002    6904    7056 
   Size:         82    5920     902     152

  Fragments arranged by size: 

                5920     902     152      82
 
EcoRI G'AATT_C

Cuts at:      0    5124    5817    5940    7056 
   Size:       5124     693     123    1116

  Fragments arranged by size: 

                5124    1116     693     123
 
EcoRV GAT'ATC

Cuts at:      0    4457    7056 
   Size:       4457    2599
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     477    1074    1913    3823    5237    7056 
   Size:        477     597     839    1910    1414    1819

  Fragments arranged by size: 

                1910    1819    1414     839     597     477
 
HaeII r_GCGC'y

Cuts at:      0    2506    7056 
   Size:       2506    4550
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     382     414     486     518     923     955    1559    1591 
   Size:        382      32      72      32     405      32     604      32

Cuts at:   1591    2397    2429    2953    2985    3396    3428    3828    3860 
   Size:        806      32     524      32     411      32     400      32

Cuts at:   3860    4140    4172    4225    4257    4614    4646    5422    5454 
   Size:        280      32      53      32     357      32     776      32

Cuts at:   5454    5643    5675    6672    6704    7056 
   Size:        189      32     997      32     352

  Fragments arranged by size: 

                 997     806     776     604     524     411     405     400
                 382     357     352     280     189      72      53      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     611    2201    4361    6080    6380    7056 
   Size:        611    1590    2160    1719     300     676

  Fragments arranged by size: 

                2160    1719    1590     676     611     300
 
HindIII A'AGCT_T

Cuts at:      0     527    3805    7056 
   Size:        527    3278    3251

  Fragments arranged by size: 

                3278    3251     527
 
HpaI GTT'AAC

Cuts at:      0    2201    4361    7056 
   Size:       2201    2160    2695

  Fragments arranged by size: 

                2695    2201    2160
 
MfeI C'AATT_G

Cuts at:      0    4606    7056 
   Size:       4606    2450
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     677    2434    3643    7056 
   Size:        614      63    1757    1209    3413

  Fragments arranged by size: 

                3413    1757    1209     614      63
 
MscI TGG'CCA

Cuts at:      0    1334    2123    2528    7056 
   Size:       1334     789     405    4528

  Fragments arranged by size: 

                4528    1334     789     405
 
MslI CAynn'nnrTG

Cuts at:      0     872    1190    1829    2129    2633    2783    3284    3344 
   Size:        872     318     639     300     504     150     501      60

Cuts at:   3344    5251    6794    7056 
   Size:       1907    1543     262

  Fragments arranged by size: 

                1907    1543     872     639     504     501     318     300
                 262     150      60
 
MspA1I CmG'CkG

Cuts at:      0      91     284    1367    2623    2941    4109    4168    6337 
   Size:         91     193    1083    1256     318    1168      59    2169

Cuts at:   6337    7056 
   Size:        719

  Fragments arranged by size: 

                2169    1256    1168    1083     719     318     193      91
                  59
 
NcoI C'CATG_G

Cuts at:      0    2258    7056 
   Size:       2258    4798
 
NdeI CA'TA_TG

Cuts at:      0    1017    1806    6231    7040    7056 
   Size:       1017     789    4425     809      16

  Fragments arranged by size: 

                4425    1017     809     789      16
 
NheI G'CTAG_C

Cuts at:      0     960    1471    3321    7056 
   Size:        960     511    1850    3735

  Fragments arranged by size: 

                3735    1850     960     511
 
NsiI A_TGCA'T

Cuts at:      0     869    7056 
   Size:        869    6187
 
NspI r_CATG'y

Cuts at:      0     871     940    1138    1386    1427    1438    4924    6799 
   Size:        871      69     198     248      41      11    3486    1875

Cuts at:   6799    7056 
   Size:        257

  Fragments arranged by size: 

                3486    1875     871     257     248     198      69      41
                  11
 
PasI CC'CwG_GG

Cuts at:      0    6676    7013    7056 
   Size:       6676     337      43

  Fragments arranged by size: 

                6676     337      43
 
PciI A'CATG_T

Cuts at:      0     936    1134    7056 
   Size:        936     198    5922

  Fragments arranged by size: 

                5922     936     198
 
PfoI T'CCnGG_A

Cuts at:      0    5580    7056 
   Size:       5580    1476
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1751    1783    6555    6587    7056 
   Size:       1751      32    4772      32     469

  Fragments arranged by size: 

                4772    1751     469      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    6904    7056 
   Size:       6904     152
 
PshAI GACnn'nnGTC

Cuts at:      0    6708    7056 
   Size:       6708     348
 
PspOMI G'GGCC_C

Cuts at:      0      81    6001    7056 
   Size:         81    5920    1055

  Fragments arranged by size: 

                5920    1055      81
 
PspXI vC'TCGA_Gb

Cuts at:      0    2759    7056 
   Size:       2759    4297
 
PstI C_TGCA'G

Cuts at:      0      42     546    1758    4114    6807    6933    7056 
   Size:         42     504    1212    2356    2693     126     123

  Fragments arranged by size: 

                2693    2356    1212     504     126     123      42
 
PvuII CAG'CTG

Cuts at:      0    2623    2941    4109    4168    6337    7056 
   Size:       2623     318    1168      59    2169     719

  Fragments arranged by size: 

                2623    2169    1168     719     318      59
 
SacI G_AGCT'C

Cuts at:      0     135    3279    7056 
   Size:        135    3144    3777

  Fragments arranged by size: 

                3777    3144     135
 
SacII CC_GC'GG

Cuts at:      0      92    7056 
   Size:         92    6964
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2337    7056 
   Size:       2337    4719
 
SbfI CC_TGCA'GG

Cuts at:      0    6807    7056 
   Size:       6807     249
 
ScaI AGT'ACT

Cuts at:      0     599    7056 
   Size:        599    6457
 
SfcI C'TryA_G

Cuts at:      0      38     542    1754    4110    4763    6803    6929    7056 
   Size:         38     504    1212    2356     653    2040     126     127

  Fragments arranged by size: 

                2356    2040    1212     653     504     127     126      38
            
 
SmlI C'TyrA_G

Cuts at:      0    1523    1598    1715    2759    3201    3791    5916    7056 
   Size:       1523      75     117    1044     442     590    2125    1140

  Fragments arranged by size: 

                2125    1523    1140    1044     590     442     117      75
            
 
SnaBI TAC'GTA

Cuts at:      0    3292    7056 
   Size:       3292    3764
 
SphI G_CATG'C

Cuts at:      0     871    7056 
   Size:        871    6185
 
SspI AAT'ATT

Cuts at:      0    2648    2849    7056 
   Size:       2648     201    4207

  Fragments arranged by size: 

                4207    2648     201
 
StuI AGG'CCT

Cuts at:      0    4869    5037    7056 
   Size:       4869     168    2019

  Fragments arranged by size: 

                4869    2019     168
 
StyI C'CwwG_G

Cuts at:      0    2258    2693    2711    3030    4963    6318    7056 
   Size:       2258     435      18     319    1933    1355     738

  Fragments arranged by size: 

                2258    1933    1355     738     435     319      18
 
SwaI ATTT'AAAT

Cuts at:      0    2222    7056 
   Size:       2222    4834
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    5331    6720    7056 
   Size:       5331    1389     336

  Fragments arranged by size: 

                5331    1389     336
 
TatI w'GTAC_w

Cuts at:      0     597    1930    1998    3570    4315    5920    5946    6757 
   Size:        597    1333      68    1572     745    1605      26     811

Cuts at:   6757    7056 
   Size:        299

  Fragments arranged by size: 

                1605    1572    1333     811     745     597     299      68
                  26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1949    1981    2298    2330    5004    5036    5210    5242 
   Size:       1949      32     317      32    2674      32     174      32

Cuts at:   5242    5505    5537    7056 
   Size:        263      32    1519

  Fragments arranged by size: 

                2674    1949    1519     317     263     174      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     615    7056 
   Size:        615    6441
 
XbaI T'CTAG_A

Cuts at:      0    2878    5413    7056 
   Size:       2878    2535    1643

  Fragments arranged by size: 

                2878    2535    1643
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1093    7056 
   Size:       1093    5963
 
XhoI C'TCGA_G

Cuts at:      0    1523    2759    7056 
   Size:       1523    1236    4297

  Fragments arranged by size: 

                4297    1523    1236
 
XmnI GAAnn'nnTTC

Cuts at:      0     599    3986    4655    7056 
   Size:        599    3387     669    2401

  Fragments arranged by size: 

                3387    2401     669     599
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI    AflII   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
    BssSI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NsiI     NspI     PasI     PciI     PfoI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PstI    PvuII     SacI    SacII     SapI
     SbfI     ScaI     SfcI     SmlI    SnaBI     SphI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI     AgeI     AhdI     AscI    AsiSI    AvrII
     BaeI     BbeI     BglI     BlpI    BmgBI    BsaHI    BsiEI    BsiWI
    BspEI    BsrBI    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I
     ClaI     DrdI     EagI     FseI     FspI    FspAI     KasI     KpnI
     MluI     NaeI     NarI   NgoMIV     NotI     NruI     PacI    PflMI
     PmeI     PmlI     PsiI     PsrI     PvuI    RsrII     SalI    SanDI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI     SrfI
     XmaI     ZraI
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