Restriction map for pF1KA0708
 (Linear) (Six-base) MAPSORT of: KIAA0708.seq  Check: 5712  from: 1  to: 7551



KIAA0708.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    6349    6547    7551 
   Size:       6349     198    1004

  Fragments arranged by size: 

                6349    1004     198
 
AccI GT'mk_AC

Cuts at:      0     215    1264    1747    6629    7551 
   Size:        215    1049     483    4882     922

  Fragments arranged by size: 

                4882    1049     922     483     215
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     154     250     468     728    1325    2413    4323 
   Size:         86      68      96     218     260     597    1088    1910

Cuts at:   4323    4705    5401    7285    7551 
   Size:        382     696    1884     266

  Fragments arranged by size: 

                1910    1884    1088     696     597     382     266     260
                 218      96      86      68
 
AfeI AGC'GCT

Cuts at:      0     784    1096    3838    6703    7551 
   Size:        784     312    2742    2865     848

  Fragments arranged by size: 

                2865    2742     848     784     312
 
AflIII A'CryG_T

Cuts at:      0     518    2684    3940    7551 
   Size:        518    2166    1256    3611

  Fragments arranged by size: 

                3611    2166    1256     518
 
AhdI GACnn_n'nnGTC

Cuts at:      0    6029    7551 
   Size:       6029    1522
 
AleI CACnn'nnGTG

Cuts at:      0    3632    4634    5486    7551 
   Size:       3632    1002     852    2065

  Fragments arranged by size: 

                3632    2065    1002     852
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     583     757    4073    7145    7246    7292    7551 
   Size:        583     174    3316    3072     101      46     259

  Fragments arranged by size: 

                3316    3072     583     259     174     101      46
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     886     918    7551 
   Size:        886      32    6633

  Fragments arranged by size: 

                6633     886      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363     576    1348    1935    2253    3168    3285    4098 
   Size:        363     213     772     587     318     915     117     813

Cuts at:   4098    4908    5304    5844    5944    6114    6522    7014    7551 
   Size:        810     396     540     100     170     408     492     537

  Fragments arranged by size: 

                 915     813     810     772     587     540     537     492
                 408     396     363     318     213     170     117     100
            
 
ApaI G_GGCC'C

Cuts at:      0     470     960    1315    2875    3020    3892    4482    5516 
   Size:        470     490     355    1560     145     872     590    1034

Cuts at:   5516    5876    7551 
   Size:        360    1675

  Fragments arranged by size: 

                1675    1560    1034     872     590     490     470     360
                 355     145
 
ApaLI G'TGCA_C

Cuts at:      0    1285    6477    6897    7551 
   Size:       1285    5192     420     654

  Fragments arranged by size: 

                5192    1285     654     420
 
ApoI r'AATT_y

Cuts at:      0    1102    4053    5167    5341    7551 
   Size:       1102    2951    1114     174    2210

  Fragments arranged by size: 

                2951    2210    1114    1102     174
 
AvaI C'yCGr_G

Cuts at:      0     302    2520    3863    4259    4553    7420    7551 
   Size:        302    2218    1343     396     294    2867     131

  Fragments arranged by size: 

                2867    2218    1343     396     302     294     131
 
AvrII C'CTAG_G

Cuts at:      0    1599    7551 
   Size:       1599    5952
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1752    1785    7551 
   Size:       1752      33    5766

  Fragments arranged by size: 

                5766    1752      33
 
BamHI G'GATC_C

Cuts at:      0    7241    7551 
   Size:       7241     310
 
BanI G'GyrC_C

Cuts at:      0      33    2935    4001    4374    4594    4727    6743    7551 
   Size:         33    2902    1066     373     220     133    2016     808

  Fragments arranged by size: 

                2902    2016    1066     808     373     220     133      33
            
 
BanII G_rGCy'C

Cuts at:      0     470     501     606     960    1315    1874    2105    2527 
   Size:        470      31     105     354     355     559     231     422

Cuts at:   2527    2875    3020    3116    3478    3746    3892    4372    4482 
   Size:        348     145      96     362     268     146     480     110

Cuts at:   4482    4695    5516    5867    5876    5995    6226    7551 
   Size:        213     821     351       9     119     231    1325

  Fragments arranged by size: 

                1325     821     559     480     470     422     362     355
                 354     351     348     268     231     231     213     146
                 145     119     110     105      96      31       9
 
BbeI G_GCGC'C

Cuts at:      0    4005    4378    4598    7551 
   Size:       4005     373     220    2953

  Fragments arranged by size: 

                4005    2953     373     220
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     474    1077    3410    5614    7291    7406    7551 
   Size:        474     603    2333    2204    1677     115     145

  Fragments arranged by size: 

                2333    2204    1677     603     474     145     115
 
BbvCI CC'TCA_GC

Cuts at:      0     967    3046    7213    7551 
   Size:        967    2079    4167     338

  Fragments arranged by size: 

                4167    2079     967     338
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3913    3947    7551 
   Size:       3913      34    3604

  Fragments arranged by size: 

                3913    3604      34
 
BclI T'GATC_A

Cuts at:      0    5670    7551 
   Size:       5670    1881
 
BfrBI ATG'CAT

Cuts at:      0    3201    7551 
   Size:       3201    4350
 
BglI GCCn_nnn'nGGC

Cuts at:      0      42    1118    2921    3134    3848    4093    5871    6518 
   Size:         42    1076    1803     213     714     245    1778     647

Cuts at:   6518    7327    7425    7551 
   Size:        809      98     126

  Fragments arranged by size: 

                1803    1778    1076     809     714     647     245     213
                 126      98      42
 
BglII A'GATC_T

Cuts at:      0    1607    1700    2489    7551 
   Size:       1607      93     789    5062

  Fragments arranged by size: 

                5062    1607     789      93
 
BlpI GC'TnA_GC

Cuts at:      0    3278    4429    5279    6071    7551 
   Size:       3278    1151     850     792    1480

  Fragments arranged by size: 

                3278    1480    1151     850     792
 
Bme1580I G_kGCm'C

Cuts at:      0      38     252     470     960    1289    1315    1384    2875 
   Size:         38     214     218     490     329      26      69    1491

Cuts at:   2875    3020    3892    3966    4179    4482    5429    5516    5876 
   Size:        145     872      74     213     303     947      87     360

Cuts at:   5876    6481    6901    6971    7421    7551 
   Size:        605     420      70     450     130

  Fragments arranged by size: 

                1491     947     872     605     490     450     420     360
                 329     303     218     214     213     145     130      87
                  74      70      69      38      26
 
BmgBI CAC'GTC

Cuts at:      0     609     800    5264    7551 
   Size:        609     191    4464    2287

  Fragments arranged by size: 

                4464    2287     609     191
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1290    3588    4108    4413    6105    7551 
   Size:       1290    2298     520     305    1692    1446

  Fragments arranged by size: 

                2298    1692    1446    1290     520     305
 
BmtI G_CTAG'C

Cuts at:      0    6605    7551 
   Size:       6605     946
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     620    1931    2604    3267    3543    6432    6477    7551 
   Size:        620    1311     673     663     276    2889      45    1074

  Fragments arranged by size: 

                2889    1311    1074     673     663     620     276      45
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700     863     919    1092    1132    1321    1426    2947 
   Size:        700     163      56     173      40     189     105    1521

Cuts at:   2947    3342    3462    3993    3994    4718    4858    4939    5178 
   Size:        395     120     531       1     724     140      81     239

Cuts at:   5178    5398    5713    5843    7036    7135    7168    7551 
   Size:        220     315     130    1193      99      33     383

  Fragments arranged by size: 

                1521    1193     724     700     531     395     383     315
                 239     220     189     173     163     140     130     120
                 105      99      81      56      40      33       1
 
Bpu10I CC'TnA_GC

Cuts at:      0     533     967    2792    2843    3046    3893    4319    4816 
   Size:        533     434    1825      51     203     847     426     497

Cuts at:   4816    4909    6137    6179    7213    7551 
   Size:         93    1228      42    1034     338

  Fragments arranged by size: 

                1825    1228    1034     847     533     497     434     426
                 338     203      93      51      42
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2938    3663    4254    5634    6781    7551 
   Size:       2938     725     591    1380    1147     770

  Fragments arranged by size: 

                2938    1380    1147     770     725     591
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     839    1573    1885    2648    2803    2965    4210    5365 
   Size:        839     734     312     763     155     162    1245    1155

Cuts at:   5365    7166    7551 
   Size:       1801     385

  Fragments arranged by size: 

                1801    1245    1155     839     763     734     385     312
                 162     155
 
BsaAI yAC'GTr

Cuts at:      0     441    5814    7551 
   Size:        441    5373    1737

  Fragments arranged by size: 

                5373    1737     441
 
BsaHI Gr'CG_yC

Cuts at:      0    4002    4375    4595    7551 
   Size:       4002     373     220    2956

  Fragments arranged by size: 

                4002    2956     373     220
 
BsaWI w'CCGG_w

Cuts at:      0    4364    6373    7551 
   Size:       4364    2009    1178

  Fragments arranged by size: 

                4364    2009    1178
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     838     868    1118    1148    1780    1810    2924    2954 
   Size:        838      30     250      30     632      30    1114      30

Cuts at:   2954    3340    3370    3487    3517    3913    3943    5176    5206 
   Size:        386      30     117      30     396      30    1233      30

Cuts at:   5206    5521    5551    6859    6889    7165    7195    7453    7483 
   Size:        315      30    1308      30     276      30     258      30

Cuts at:   7483    7551 
   Size:         68

  Fragments arranged by size: 

                1308    1233    1114     838     632     396     386     315
                 276     258     250     117      68      30      30      30
                  30      30      30      30      30      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1201    1801    2143    2406    2530    2941    3997    4292 
   Size:       1201     600     342     263     124     411    1056     295

Cuts at:   4292    4936    5008    5032    5035    5044    5371    6390    7173 
   Size:        644      72      24       3       9     327    1019     783

Cuts at:   7173    7359    7405    7408    7462    7551 
   Size:        186      46       3      54      89

  Fragments arranged by size: 

                1201    1056    1019     783     644     600     411     342
                 327     295     263     186     124      89      72      54
                  46      24       9       3       3
 
BseYI C'CCAG_C

Cuts at:      0      19     280     592     834     953    1033    1198    2377 
   Size:         19     261     312     242     119      80     165    1179

Cuts at:   2377    2975    3288    3390    3506    4112    4710    5268    5516 
   Size:        598     313     102     116     606     598     558     248

Cuts at:   5516    5746    5932    6157    6365    6554    6709    7551 
   Size:        230     186     225     208     189     155     842

  Fragments arranged by size: 

                1179     842     606     598     598     558     313     312
                 261     248     242     230     225     208     189     186
                 165     155     119     116     102      80      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575    1156    1267    2083    2266    2389    2819    3604 
   Size:        575     581     111     816     183     123     430     785

Cuts at:   3604    4159    4459    5341    5776    6058    6733    7105    7188 
   Size:        555     300     882     435     282     675     372      83

Cuts at:   7188    7551 
   Size:        363

  Fragments arranged by size: 

                 882     816     785     675     581     575     555     435
                 430     372     363     300     282     183     123     111
                  83
 
BsiEI CG_ry'CG

Cuts at:      0     419    2051    2296    6501    7551 
   Size:        419    1632     245    4205    1050

  Fragments arranged by size: 

                4205    1632    1050     419     245
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     446    1289    2590    3224    3746    4520    4784 
   Size:        185     261     843    1301     634     522     774     264

Cuts at:   4784    4915    6481    6901    7551 
   Size:        131    1566     420     650

  Fragments arranged by size: 

                1566    1301     843     774     650     634     522     420
                 264     261     185     131
 
BsiWI C'GTAC_G

Cuts at:      0    6192    7551 
   Size:       6192    1359
 
BsmI GAATG_Cn'

Cuts at:      0     734    1235    3377    7272    7551 
   Size:        734     501    2142    3895     279

  Fragments arranged by size: 

                3895    2142     734     501     279
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4816    5124    6037    6402    7551 
   Size:       4816     308     913     365    1149

  Fragments arranged by size: 

                4816    1149     913     365     308
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     185     252     446     470     501     606     960 
   Size:         38     147      67     194      24      31     105     354

Cuts at:    960    1289    1315    1384    1874    2105    2527    2590    2875 
   Size:        329      26      69     490     231     422      63     285

Cuts at:   2875    3020    3116    3224    3478    3746    3892    3966    4179 
   Size:        145      96     108     254     268     146      74     213

Cuts at:   4179    4372    4482    4520    4695    4784    4915    5429    5516 
   Size:        193     110      38     175      89     131     514      87

Cuts at:   5516    5867    5876    5995    6226    6481    6901    6971    7421 
   Size:        351       9     119     231     255     420      70     450

Cuts at:   7421    7551 
   Size:        130

  Fragments arranged by size: 

                 514     490     450     422     420     354     351     329
                 285     268     255     254     231     231     213     194
                 193     175     147     146     145     131     130     119
                 110     108     105      96      89      87      74      70
                  69      67      63      38      38      31      26      24
                   9
 
BspHI T'CATG_A

Cuts at:      0    2273    2750    5502    7551 
   Size:       2273     477    2752    2049

  Fragments arranged by size: 

                2752    2273    2049     477
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     515    1239    1635    1694    2096    2276    3181    4117 
   Size:        515     724     396      59     402     180     905     936

Cuts at:   4117    4437    6254    6349    6547    7551 
   Size:        320    1817      95     198    1004

  Fragments arranged by size: 

                1817    1004     936     905     724     515     402     396
                 320     198     180      95      59
 
BsrBI CCG'CTC

Cuts at:      0     246    2142    2298    5857    7551 
   Size:        246    1896     156    3559    1694

  Fragments arranged by size: 

                3559    1896    1694     246     156
 
BsrDI GCAATG_nn'

Cuts at:      0     338     673    3089    6347    7551 
   Size:        338     335    2416    3258    1204

  Fragments arranged by size: 

                3258    2416    1204     338     335
 
BsrFI r'CCGG_y

Cuts at:      0    3370    3598    3998    5831    7551 
   Size:       3370     228     400    1833    1720

  Fragments arranged by size: 

                3370    1833    1720     400     228
 
BsrGI T'GTAC_A

Cuts at:      0    7052    7551 
   Size:       7052     499
 
BssSI C'ACGA_G

Cuts at:      0    2284    3100    4045    5422    7551 
   Size:       2284     816     945    1377    2129

  Fragments arranged by size: 

                2284    2129    1377     945     816
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2253    3728    4109    5304    6027    6509    7551 
   Size:       2253    1475     381    1195     723     482    1042

  Fragments arranged by size: 

                2253    1475    1195    1042     723     482     381
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     387     592     634    1989    2223    3261    3366    3702 
   Size:        387     205      42    1355     234    1038     105     336

Cuts at:   3702    5111    5524    7551 
   Size:       1409     413    2027

  Fragments arranged by size: 

                2027    1409    1355    1038     413     387     336     234
                 205     105      42
 
BstYI r'GATC_y

Cuts at:      0     306     542     749    1304    1607    1700    1776    2423 
   Size:        306     236     207     555     303      93      76     647

Cuts at:   2423    2489    3245    3824    4667    5067    5840    6979    7139 
   Size:         66     756     579     843     400     773    1139     160

Cuts at:   7139    7241    7551 
   Size:        102     310

  Fragments arranged by size: 

                1139     843     773     756     647     579     555     400
                 310     306     303     236     207     160     102      93
                  76      66
 
Bsu36I CC'TnA_GG

Cuts at:      0      39     209    1316    3077    3457    4405    5436    6584 
   Size:         39     170    1107    1761     380     948    1031    1148

Cuts at:   6584    7371    7551 
   Size:        787     180

  Fragments arranged by size: 

                1761    1148    1107    1031     948     787     380     180
                 170      39
 
BtgI C'CryG_G

Cuts at:      0    1619    1898    3630    5646    5867    6774    6842    7551 
   Size:       1619     279    1732    2016     221     907      68     709

  Fragments arranged by size: 

                2016    1732    1619     907     709     279     221      68
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3169    7551 
   Size:       3169    4382
 
BtsI GCAGTG_nn'

Cuts at:      0     714    3150    4587    4619    5263    5529    6142    6204 
   Size:        714    2436    1437      32     644     266     613      62

Cuts at:   6204    7551 
   Size:       1347

  Fragments arranged by size: 

                2436    1437    1347     714     644     613     266      62
                  32
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    4758    4793    7551 
   Size:       4758      35    2758

  Fragments arranged by size: 

                4758    2758      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1909    7551 
   Size:       1909    5642
 
EaeI y'GGCC_r

Cuts at:      0     377     416     994    1628    1991    2054    2293    2402 
   Size:        377      39     578     634     363      63     239     109

Cuts at:   2402    2442    3368    3665    3907    4766    6609    6683    7551 
   Size:         40     926     297     242     859    1843      74     868

  Fragments arranged by size: 

                1843     926     868     859     634     578     377     363
                 297     242     239     109      74      63      40      39
            
 
EagI C'GGCC_G

Cuts at:      0     416    2293    7551 
   Size:        416    1877    5258

  Fragments arranged by size: 

                5258    1877     416
 
EarI CTCTTCn'nnn_

Cuts at:      0    1778    1814    2595    3952    4951    5009    5021    5030 
   Size:       1778      36     781    1357     999      58      12       9

Cuts at:   5030    5596    7382    7520    7551 
   Size:        566    1786     138      31

  Fragments arranged by size: 

                1786    1778    1357     999     781     566     138      58
                  36      31      12       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     535    1078    4819    7254    7551 
   Size:        535     543    3741    2435     297

  Fragments arranged by size: 

                3741    2435     543     535     297
 
EcoICRI GAG'CTC

Cuts at:      0    3744    7551 
   Size:       3744    3807
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     154     250     468     700     728     863     919 
   Size:         86      68      96     218     232      28     135      56

Cuts at:    919    1092    1132    1321    1325    1426    2413    2947    3342 
   Size:        173      40     189       4     101     987     534     395

Cuts at:   3342    3462    3993    3994    4323    4705    4718    4858    4939 
   Size:        120     531       1     329     382      13     140      81

Cuts at:   4939    5178    5398    5401    5713    5843    7036    7135    7168 
   Size:        239     220       3     312     130    1193      99      33

Cuts at:   7168    7285    7551 
   Size:        117     266

  Fragments arranged by size: 

                1193     987     534     531     395     382     329     312
                 266     239     232     220     218     189     173     140
                 135     130     120     117     101      99      96      86
                  81      68      56      40      33      28      13       4
                   3       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2116    2965    5461    7147    7551 
   Size:       2116     849    2496    1686     404

  Fragments arranged by size: 

                2496    2116    1686     849     404
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24      43     401     467    1030    1312    1596    1851 
   Size:         24      19     358      66     563     282     284     255

Cuts at:   1851    2008    2250    2837    2871    2945    3016    3306    3882 
   Size:        157     242     587      34      74      71     290     576

Cuts at:   3882    3889    4119    4478    4479    5513    5532    5658    5872 
   Size:          7     230     359       1    1034      19     126     214

Cuts at:   5872    5873    6132    6519    7336    7551 
   Size:          1     259     387     817     215

  Fragments arranged by size: 

                1034     817     587     576     563     387     359     358
                 290     284     282     259     255     242     230     215
                 214     157     126      74      71      66      34      24
                  19      19       7       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1102    5167    7551 
   Size:       1102    4065    2384

  Fragments arranged by size: 

                4065    2384    1102
 
EcoRV GAT'ATC

Cuts at:      0    1477    2069    7551 
   Size:       1477     592    5482

  Fragments arranged by size: 

                5482    1477     592
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1545    4992    7551 
   Size:       1545    3447    2559

  Fragments arranged by size: 

                3447    2559    1545
 
FspI TGC'GCA

Cuts at:      0    4579    7551 
   Size:       4579    2972
 
HaeII r_GCGC'y

Cuts at:      0     364     786    1098    2961    3840    4005    4378    4598 
   Size:        364     422     312    1863     879     165     373     220

Cuts at:   4598    4935    6104    6436    6705    6792    7551 
   Size:        337    1169     332     269      87     759

  Fragments arranged by size: 

                1863    1169     879     759     422     373     364     337
                 332     312     269     220     165      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     113     145     838     870    1780    1812    1813    1845 
   Size:        113      32     693      32     910      32       1      32

Cuts at:   1845    2438    2470    3256    3288    5176    5208    5347    5379 
   Size:        593      32     786      32    1888      32     139      32

Cuts at:   5379    5764    5796    6015    6047    6235    6267    7165    7197 
   Size:        385      32     219      32     188      32     898      32

Cuts at:   7197    7453    7485    7551 
   Size:        256      32      66

  Fragments arranged by size: 

                1888     910     898     786     693     593     385     256
                 219     188     139     113      66      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32       1
 
HincII GTy'rAC

Cuts at:      0    1265    6630    7551 
   Size:       1265    5365     921

  Fragments arranged by size: 

                5365    1265     921
 
HindIII A'AGCT_T

Cuts at:      0     294    7551 
   Size:        294    7257
 
KasI G'GCGC_C

Cuts at:      0    4001    4374    4594    7551 
   Size:       4001     373     220    2957

  Fragments arranged by size: 

                4001    2957     373     220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1505    1778    3554    3611    6488    7551 
   Size:       1505     273    1776      57    2877    1063

  Fragments arranged by size: 

                2877    1776    1505    1063     273      57
 
MscI TGG'CCA

Cuts at:      0    1993    2056    3667    3909    4768    6611    6685    7551 
   Size:       1993      63    1611     242     859    1843      74     866

  Fragments arranged by size: 

                1993    1843    1611     866     859     242      74      63
            
 
MslI CAynn'nnrTG

Cuts at:      0     190     439     517     913    1966    2749    3382    3632 
   Size:        190     249      78     396    1053     783     633     250

Cuts at:   3632    4634    5486    6658    7087    7127    7222    7551 
   Size:       1002     852    1172     429      40      95     329

  Fragments arranged by size: 

                1172    1053    1002     852     783     633     429     396
                 329     250     249     190      95      78      40
 
MspA1I CmG'CkG

Cuts at:      0     280     387     421     816     891    1348    1920    2047 
   Size:        280     107      34     395      75     457     572     127

Cuts at:   2047    3282    3728    3927    4725    4893    4959    5676    5970 
   Size:       1235     446     199     798     168      66     717     294

Cuts at:   5970    6111    6157    6713    7551 
   Size:        141      46     556     838

  Fragments arranged by size: 

                1235     838     798     717     572     556     457     446
                 395     294     280     199     168     141     127     107
                  75      66      46      34
 
NaeI GCC'GGC

Cuts at:      0    3372    7551 
   Size:       3372    4179
 
NarI GG'CG_CC

Cuts at:      0    4002    4375    4595    7551 
   Size:       4002     373     220    2956

  Fragments arranged by size: 

                4002    2956     373     220
 
NcoI C'CATG_G

Cuts at:      0    1898    5646    5867    6774    6842    7551 
   Size:       1898    3748     221     907      68     709

  Fragments arranged by size: 

                3748    1898     907     709     221      68
 
NdeI CA'TA_TG

Cuts at:      0    6614    7551 
   Size:       6614     937
 
NgoMIV G'CCGG_C

Cuts at:      0    3370    7551 
   Size:       3370    4181
 
NheI G'CTAG_C

Cuts at:      0    6601    7551 
   Size:       6601     950
 
NotI GC'GGCC_GC

Cuts at:      0    2293    7551 
   Size:       2293    5258
 
NsiI A_TGCA'T

Cuts at:      0    3203    7551 
   Size:       3203    4348
 
NspI r_CATG'y

Cuts at:      0      19     522     984    1299    2041    2343    3627    3690 
   Size:         19     503     462     315     742     302    1284      63

Cuts at:   3690    3944    4294    5311    6267    7347    7551 
   Size:        254     350    1017     956    1080     204

  Fragments arranged by size: 

                1284    1080    1017     956     742     503     462     350
                 315     302     254     204      63      19
 
PasI CC'CwG_GG

Cuts at:      0     229    1584    4179    5730    6228    6489    7551 
   Size:        229    1355    2595    1551     498     261    1062

  Fragments arranged by size: 

                2595    1551    1355    1062     498     261     229
 
PciI A'CATG_T

Cuts at:      0     518    3940    7551 
   Size:        518    3422    3611

  Fragments arranged by size: 

                3611    3422     518
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1935    2154    2649    3905    4098    4284    6522    7014 
   Size:       1935     219     495    1256     193     186    2238     492

Cuts at:   7014    7551 
   Size:        537

  Fragments arranged by size: 

                2238    1935    1256     537     495     492     219     193
                 186
 
PfoI T'CCnGG_A

Cuts at:      0     309     806    2891    3260    3917    5228    5721    6802 
   Size:        309     497    2085     369     657    1311     493    1081

Cuts at:   6802    7145    7244    7551 
   Size:        343      99     307

  Fragments arranged by size: 

                2085    1311    1081     657     497     493     369     343
                 309     307      99
 
PmlI CAC'GTG

Cuts at:      0     441    7551 
   Size:        441    7110
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1198    1230    4135    4167    4809    4841    7551 
   Size:       1198      32    2905      32     642      32    2710

  Fragments arranged by size: 

                2905    2710    1198     642      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      24     401    2008    2837    3306    3882    6132    7336 
   Size:         24     377    1607     829     469     576    2250    1204

Cuts at:   7336    7551 
   Size:        215

  Fragments arranged by size: 

                2250    1607    1204     829     576     469     377     215
                  24
 
PshAI GACnn'nnGTC

Cuts at:      0    2359    7551 
   Size:       2359    5192
 
PsiI TTA'TAA

Cuts at:      0    2665    7551 
   Size:       2665    4886
 
PspOMI G'GGCC_C

Cuts at:      0     466     956    1311    2871    3016    3888    4478    5512 
   Size:        466     490     355    1560     145     872     590    1034

Cuts at:   5512    5872    7551 
   Size:        360    1679

  Fragments arranged by size: 

                1679    1560    1034     872     590     490     466     360
                 355     145
 
PstI C_TGCA'G

Cuts at:      0      56     572    1646    2093    2556    2598    2883    4470 
   Size:         56     516    1074     447     463      42     285    1587

Cuts at:   4470    4607    4904    4931    5258    5288    5979    6119    7139 
   Size:        137     297      27     327      30     691     140    1020

Cuts at:   7139    7271    7551 
   Size:        132     280

  Fragments arranged by size: 

                1587    1074    1020     691     516     463     447     327
                 297     285     280     140     137     132      56      42
                  30      27
 
PvuII CAG'CTG

Cuts at:      0     280     387     816    1348    1920    3282    3728    3927 
   Size:        280     107     429     532     572    1362     446     199

Cuts at:   3927    4893    5676    5970    6111    6157    6713    7551 
   Size:        966     783     294     141      46     556     838

  Fragments arranged by size: 

                1362     966     838     783     572     556     532     446
                 429     294     280     199     141     107      46
 
RsrII CG'GwC_CG

Cuts at:      0    4806    7551 
   Size:       4806    2745
 
SacI G_AGCT'C

Cuts at:      0    3746    7551 
   Size:       3746    3805
 
SalI G'TCGA_C

Cuts at:      0    1263    6628    7551 
   Size:       1263    5365     923

  Fragments arranged by size: 

                5365    1263     923
 
SanDI GG'GwC_CC

Cuts at:      0     401    2008    2837    7551 
   Size:        401    1607     829    4714

  Fragments arranged by size: 

                4714    1607     829     401
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2595    7551 
   Size:       2595    4956
 
SbfI CC_TGCA'GG

Cuts at:      0    5979    7551 
   Size:       5979    1572
 
ScaI AGT'ACT

Cuts at:      0    4201    7551 
   Size:       4201    3350
 
SexAI A'CCwGG_T

Cuts at:      0    2219    3308    6472    7551 
   Size:       2219    1089    3164    1079

  Fragments arranged by size: 

                3164    2219    1089    1079
 
SfcI C'TryA_G

Cuts at:      0      52     568    1642    2089    2552    2594    2879    3342 
   Size:         52     516    1074     447     463      42     285     463

Cuts at:   3342    4466    4603    4900    4927    5202    5254    5284    5965 
   Size:       1124     137     297      27     275      52      30     681

Cuts at:   5965    5975    6115    6123    6573    7062    7135    7267    7551 
   Size:         10     140       8     450     489      73     132     284

  Fragments arranged by size: 

                1124    1074     681     516     489     463     463     450
                 447     297     285     284     275     140     137     132
                  73      52      52      42      30      27      10       8
            
 
SfoI GGC'GCC

Cuts at:      0    4003    4376    4596    7551 
   Size:       4003     373     220    2955

  Fragments arranged by size: 

                4003    2955     373     220
 
SgrAI Cr'CCGG_yG

Cuts at:      0    3998    7551 
   Size:       3998    3553
 
SmaI CCC'GGG

Cuts at:      0    2522    3865    7551 
   Size:       2522    1343    3686

  Fragments arranged by size: 

                3686    2522    1343
 
SmlI C'TyrA_G

Cuts at:      0     302    2953    3678    4233    4259    5613    6796    7551 
   Size:        302    2651     725     555      26    1354    1183     755

  Fragments arranged by size: 

                2651    1354    1183     755     725     555     302      26
            
 
SphI G_CATG'C

Cuts at:      0      19    2343    6267    7551 
   Size:         19    2324    3924    1284

  Fragments arranged by size: 

                3924    2324    1284      19
 
SrfI GCCC'GGGC

Cuts at:      0    2522    7551 
   Size:       2522    5029
 
StuI AGG'CCT

Cuts at:      0      87     317    5698    7321    7366    7543    7551 
   Size:         87     230    5381    1623      45     177       8

  Fragments arranged by size: 

                5381    1623     230     177      87      45       8
 
StyI C'CwwG_G

Cuts at:      0     773    1599    1898    5646    5867    6774    6842    7551 
   Size:        773     826     299    3748     221     907      68     709

  Fragments arranged by size: 

                3748     907     826     773     709     299     221      68
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148    1272    1576    1903    7551 
   Size:        148    1124     304     327    5648

  Fragments arranged by size: 

                5648    1124     327     304     148
 
TatI w'GTAC_w

Cuts at:      0    4199    5767    7052    7079    7551 
   Size:       4199    1568    1285      27     472

  Fragments arranged by size: 

                4199    1568    1285     472      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1118    1150    1374    1406    2729    2761    2924    2956 
   Size:       1118      32     224      32    1323      32     163      32

Cuts at:   2956    3487    3519    3913    3945    5521    5553    7551 
   Size:        531      32     394      32    1576      32    1998

  Fragments arranged by size: 

                1998    1576    1323    1118     531     394     224     163
                  32      32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1827    7551 
   Size:       1827    5724
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2830    5088    5743    7551 
   Size:       2830    2258     655    1808

  Fragments arranged by size: 

                2830    2258    1808     655
 
XhoI C'TCGA_G

Cuts at:      0     302    4259    7551 
   Size:        302    3957    3292

  Fragments arranged by size: 

                3957    3292     302
 
XmaI C'CCGG_G

Cuts at:      0    2520    3863    7551 
   Size:       2520    1343    3688

  Fragments arranged by size: 

                3688    2520    1343
 
XmnI GAAnn'nnTTC

Cuts at:      0      74    3805    7551 
   Size:         74    3731    3746

  Fragments arranged by size: 

                3746    3731      74
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PstI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI    SgrAI     SmaI
     SmlI     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AscI     AseI    AsiSI
    BciVI    BsaBI    BspEI   BssHII    BstBI   BstEII  BstZ17I     ClaI
     DraI     DrdI     FseI    FspAI     HpaI     KpnI     MfeI     MluI
     NruI     PacI     PmeI    PspXI     PsrI     PvuI    SacII     SfiI
     SgfI    SnaBI     SpeI     SspI     SwaI     XbaI     ZraI
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