Restriction map for pF1KE1013
 (Linear) (Six-base) MAPSORT of: KIEE1013.seq  Check: 470  from: 1  to: 855



KIEE1013.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

AccI GT'mk_AC

Cuts at:      0     284     855 
   Size:        284     571
 
AflIII A'CryG_T

Cuts at:      0     362     855 
   Size:        362     493
 
AhdI GACnn_n'nnGTC

Cuts at:      0     421     855 
   Size:        421     434
 
ApaLI G'TGCA_C

Cuts at:      0     349     855 
   Size:        349     506
 
AvaI C'yCGr_G

Cuts at:      0     187     855 
   Size:        187     668
 
BanII G_rGCy'C

Cuts at:      0     650     855 
   Size:        650     205
 
BbvCI CC'TCA_GC

Cuts at:      0     613     855 
   Size:        613     242
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     528     855 
   Size:        528     327
 
Bme1580I G_kGCm'C

Cuts at:      0      51     353     497     632     855 
   Size:         51     302     144     135     223

  Fragments arranged by size: 

                 302     223     144     135      51
 
BmgBI CAC'GTC

Cuts at:      0     678     855 
   Size:        678     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     766     838     855 
   Size:        258     508      72      17

  Fragments arranged by size: 

                 508     258      72      17
 
Bpu10I CC'TnA_GC

Cuts at:      0     613     855 
   Size:        613     242
 
BsaHI Gr'CG_yC

Cuts at:      0     590     722     855 
   Size:        590     132     133

  Fragments arranged by size: 

                 590     133     132
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      26      29      49      52     220     855 
   Size:         26       3      20       3     168     635

  Fragments arranged by size: 

                 635     168      26      20       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     808     855 
   Size:         24     784      47

  Fragments arranged by size: 

                 784      47      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     353     605     650     855 
   Size:        188     165     252      45     205

  Fragments arranged by size: 

                 252     205     188     165      45
 
BsmI GAATG_Cn'

Cuts at:      0     311     855 
   Size:        311     544
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     742     855 
   Size:        742     113
 
Bsp1286I G_dGCh'C

Cuts at:      0      51     188     353     497     605     632     650     855 
   Size:         51     137     165     144     108      27      18     205

  Fragments arranged by size: 

                 205     165     144     137     108      51      27      18
            
 
BspHI T'CATG_A

Cuts at:      0     485     855 
   Size:        485     370
 
BsrBI CCG'CTC

Cuts at:      0     783     855 
   Size:        783      72
 
BsrFI r'CCGG_y

Cuts at:      0     552     855 
   Size:        552     303
 
BtsI GCAGTG_nn'

Cuts at:      0     217     855 
   Size:        217     638
 
EaeI y'GGCC_r

Cuts at:      0     582     713     855 
   Size:        582     131     142

  Fragments arranged by size: 

                 582     142     131
 
EarI CTCTTCn'nnn_

Cuts at:      0     517     755     855 
   Size:        517     238     100

  Fragments arranged by size: 

                 517     238     100
 
EcoICRI GAG'CTC

Cuts at:      0     648     855 
   Size:        648     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     766     838     855 
   Size:        258     508      72      17

  Fragments arranged by size: 

                 508     258      72      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     514     855 
   Size:        514     341
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60     136     246     771     855 
   Size:         60      76     110     525      84

  Fragments arranged by size: 

                 525     110      84      76      60
 
FspI TGC'GCA

Cuts at:      0      27     855 
   Size:         27     828
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     407     439     855 
   Size:        256      32     119      32     416

  Fragments arranged by size: 

                 416     256     119      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     490     855 
   Size:        490     365
 
MspA1I CmG'CkG

Cuts at:      0     387     855 
   Size:        387     468
 
NaeI GCC'GGC

Cuts at:      0     554     855 
   Size:        554     301
 
NgoMIV G'CCGG_C

Cuts at:      0     552     855 
   Size:        552     303
 
NspI r_CATG'y

Cuts at:      0     828     855 
   Size:        828      27
 
PasI CC'CwG_GG

Cuts at:      0      51     855 
   Size:         51     804
 
PfoI T'CCnGG_A

Cuts at:      0     515     671     855 
   Size:        515     156     184

  Fragments arranged by size: 

                 515     184     156
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     587     619     855 
   Size:        587      32     236

  Fragments arranged by size: 

                 587     236      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     136     246     855 
   Size:        136     110     609

  Fragments arranged by size: 

                 609     136     110
 
PstI C_TGCA'G

Cuts at:      0     224     855 
   Size:        224     631
 
SacI G_AGCT'C

Cuts at:      0     650     855 
   Size:        650     205
 
SanDI GG'GwC_CC

Cuts at:      0     136     855 
   Size:        136     719
 
SfcI C'TryA_G

Cuts at:      0     220     855 
   Size:        220     635
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     414     855 
   Size:        414     441
 
XmnI GAAnn'nnTTC

Cuts at:      0     199     364     855 
   Size:        199     165     491

  Fragments arranged by size: 

                 491     199     165
 

 Enzymes that do cut:

     AccI   AflIII     AhdI    ApaLI     AvaI    BanII    BbvCI    BciVI
 Bme1580I    BmgBI     BpmI   Bpu10I    BsaHI    BseRI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI     BtsI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    Hin4I     MslI
   MspA1I     NaeI   NgoMIV     NspI     PasI     PfoI     PpiI    PpuMI
     PstI     SacI    SanDI     SfcI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI    BsiEI
    BsiWI    BspEI    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoRI
    EcoRV     FalI     FseI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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