Restriction map for pF1KB9387
 (Linear) (Six-base) MAPSORT of: KIBB9387.seq  Check: 445  from: 1  to: 864



KIBB9387.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     144     864 
   Size:        144     720
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     307     339     864 
   Size:        307      32     525

  Fragments arranged by size: 

                 525     307      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     219     570     864 
   Size:        219     351     294

  Fragments arranged by size: 

                 351     294     219
 
BamHI G'GATC_C

Cuts at:      0     440     864 
   Size:        440     424
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     253     500     864 
   Size:        211      42     247     364

  Fragments arranged by size: 

                 364     247     211      42
 
BclI T'GATC_A

Cuts at:      0     797     864 
   Size:        797      67
 
BglI GCCn_nnn'nGGC

Cuts at:      0     181     864 
   Size:        181     683
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     402     864 
   Size:        402     462
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     475     523     631     864 
   Size:        241     234      48     108     233

  Fragments arranged by size: 

                 241     234     233     108      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     607     864 
   Size:        607     257
 
BsaAI yAC'GTr

Cuts at:      0      66     726     864 
   Size:         66     660     138

  Fragments arranged by size: 

                 660     138      66
 
BsaHI Gr'CG_yC

Cuts at:      0     835     864 
   Size:        835      29
 
BsaWI w'CCGG_w

Cuts at:      0     437     864 
   Size:        437     427
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     307     337     864 
   Size:        307      30     527

  Fragments arranged by size: 

                 527     307      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     319     864 
   Size:         67     252     545

  Fragments arranged by size: 

                 545     252      67
 
BseYI C'CCAG_C

Cuts at:      0     350     819     855     864 
   Size:        350     469      36       9

  Fragments arranged by size: 

                 469     350      36       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     864 
   Size:        616     248
 
BsiEI CG_ry'CG

Cuts at:      0     284     864 
   Size:        284     580
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     577     864 
   Size:        577     287
 
BspHI T'CATG_A

Cuts at:      0     794     864 
   Size:        794      70
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     144     341     864 
   Size:        144     197     523

  Fragments arranged by size: 

                 523     197     144
 
BsrBI CCG'CTC

Cuts at:      0     509     864 
   Size:        509     355
 
BstYI r'GATC_y

Cuts at:      0     113     225     440     864 
   Size:        113     112     215     424

  Fragments arranged by size: 

                 424     215     113     112
 
BtgI C'CryG_G

Cuts at:      0     326     864 
   Size:        326     538
 
BtsI GCAGTG_nn'

Cuts at:      0     230     764     864 
   Size:        230     534     100

  Fragments arranged by size: 

                 534     230     100
 
EaeI y'GGCC_r

Cuts at:      0     182     251     518     653     864 
   Size:        182      69     267     135     211

  Fragments arranged by size: 

                 267     211     182     135      69
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     104     864 
   Size:         44      60     760

  Fragments arranged by size: 

                 760      60      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     475     523     631     864 
   Size:        241     234      48     108     233

  Fragments arranged by size: 

                 241     234     233     108      48
 
FspI TGC'GCA

Cuts at:      0     337     864 
   Size:        337     527
 
HaeII r_GCGC'y

Cuts at:      0     176     571     864 
   Size:        176     395     293

  Fragments arranged by size: 

                 395     293     176
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     864 
   Size:        121      32     711

  Fragments arranged by size: 

                 711     121      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     864 
   Size:        119     745
 
MscI TGG'CCA

Cuts at:      0     520     655     864 
   Size:        520     135     209

  Fragments arranged by size: 

                 520     209     135
 
MslI CAynn'nnrTG

Cuts at:      0     541     655     864 
   Size:        541     114     209

  Fragments arranged by size: 

                 541     209     114
 
MspA1I CmG'CkG

Cuts at:      0     328     428     447     864 
   Size:        328     100      19     417

  Fragments arranged by size: 

                 417     328     100      19
 
NspI r_CATG'y

Cuts at:      0     501     864 
   Size:        501     363
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      84     864 
   Size:         84     780
 
PfoI T'CCnGG_A

Cuts at:      0     367     864 
   Size:        367     497
 
PmlI CAC'GTG

Cuts at:      0      66     864 
   Size:         66     798
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     307     339     864 
   Size:        307      32     525

  Fragments arranged by size: 

                 525     307      32
 
PstI C_TGCA'G

Cuts at:      0     237     864 
   Size:        237     627
 
PvuI CG_AT'CG

Cuts at:      0     284     864 
   Size:        284     580
 
SacII CC_GC'GG

Cuts at:      0     329     864 
   Size:        329     535
 
SfcI C'TryA_G

Cuts at:      0     233     864 
   Size:        233     631
 
SmlI C'TyrA_G

Cuts at:      0      14     864 
   Size:         14     850
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     204     254     406     864 
   Size:        204      50     152     458

  Fragments arranged by size: 

                 458     204     152      50
 
TatI w'GTAC_w

Cuts at:      0     698     864 
   Size:        698     166
 
Tth111I GACn'n_nGTC

Cuts at:      0     408     864 
   Size:        408     456
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216     486     864 
   Size:        216     270     378

  Fragments arranged by size: 

                 378     270     216
 

 Enzymes that do cut:

     AarI     AloI    AlwNI    BamHI     BbsI     BclI     BglI     BmrI
     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsmBI    BspHI    BspMI    BsrBI    BstYI     BtgI
     BtsI     EaeI     EarI  Eco57MI     FspI    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NspI    PflMI     PfoI     PmlI     PpiI
     PstI     PvuI    SacII     SfcI     SmlI    TaqII     TatI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsaBI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI
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