Restriction map for pF1KB5654
 (Circular) (Six-base) MAPSORT of: pF1KB5654.seq  Check: 3415  from: 1  to: 4106



pF1KB5654  4106 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AatII G_ACGT'C

Cuts at:    255     255 
   Size:       4106
 
AccI GT'mk_AC

Cuts at:    905    1144     905 
   Size:        239    3867
 
Acc65I G'GTAC_C

Cuts at:   1122    1122 
   Size:       4106
 
AclI AA'CG_TT

Cuts at:   3946    3946 
   Size:       4106
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    512     583    1931    2363    3110    3378     512 
   Size:         71    1348     432     747     268    1240

  Fragments arranged by size: 

                1348    1240     747     432     268      71
 
AflIII A'CryG_T

Cuts at:    775    1458    2562    3539     775 
   Size:        683    1104     977    1342

  Fragments arranged by size: 

                1342    1104     977     683
 
AgeI A'CCGG_T

Cuts at:   2494    2494 
   Size:       4106
 
AlwNI CAG_nnn'CTG

Cuts at:    606     853    1411    2978     606 
   Size:        247     558    1567    1734

  Fragments arranged by size: 

                1734    1567     558     247
 
ApaI G_GGCC'C

Cuts at:    549     549 
   Size:       4106
 
ApaLI G'TGCA_C

Cuts at:   2876    2876 
   Size:       4106
 
ApoI r'AATT_y

Cuts at:   1110    3459    3715    1110 
   Size:       2349     256    1501

  Fragments arranged by size: 

                2349    1501     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4106
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4106
 
AvaI C'yCGr_G

Cuts at:   1126    1126 
   Size:       4106
 
BamHI G'GATC_C

Cuts at:   1131    1131 
   Size:       4106
 
BanI G'GyrC_C

Cuts at:   1122    1536    1784    1819    1122 
   Size:        414     248      35    3409

  Fragments arranged by size: 

                3409     414     248      35
 
BanII G_rGCy'C

Cuts at:    549    1120    2150     549 
   Size:        571    1030    2505

  Fragments arranged by size: 

                2505    1030     571
 
BbeI G_GCGC'C

Cuts at:   1540    1788    1540 
   Size:        248    3858
 
BciVI GTATCCnnnnn_n'

Cuts at:   2004    2765    4106    2004 
   Size:        761    1341    2004

  Fragments arranged by size: 

                2004    1341     761
 
BclI T'GATC_A

Cuts at:   1626    1626 
   Size:       4106
 
BglII A'GATC_T

Cuts at:   1621    3658    1621 
   Size:       2037    2069
 
BlpI GC'TnA_GC

Cuts at:   1218    1218 
   Size:       4106
 
Bme1580I G_kGCm'C

Cuts at:    549     600    1731    1824    2880     549 
   Size:         51    1131      93    1056    1775

  Fragments arranged by size: 

                1775    1131    1056      93      51
 
BmrI ACTGGGnnnn_n'

Cuts at:    841    1318    1399    1734    3878     841 
   Size:        477      81     335    2144    1069

  Fragments arranged by size: 

                2144    1069     477     335      81
 
BmtI G_CTAG'C

Cuts at:   1079    1079 
   Size:       4106
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2473    2473 
   Size:       4106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    319     489     319 
   Size:        170    3936
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1282    2161    2653    2951    1282 
   Size:        879     492     298    2437

  Fragments arranged by size: 

                2437     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3810    3810 
   Size:       4106
 
BsaAI yAC'GTr

Cuts at:   1459    1459 
   Size:       4106
 
BsaBI GATnn'nnATC

Cuts at:      6    1620    3657       6 
   Size:       1614    2037     455

  Fragments arranged by size: 

                2037    1614     455
 
BsaHI Gr'CG_yC

Cuts at:    252    1537    1785    2487    3983     252 
   Size:       1285     248     702    1496     375

  Fragments arranged by size: 

                1496    1285     702     375     248
 
BsaWI w'CCGG_w

Cuts at:   1295    1816    2494    2768    2915    3326    1295 
   Size:        521     678     274     147     411    2075

  Fragments arranged by size: 

                2075     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    132     132 
   Size:       4106
 
BseYI C'CCAG_C

Cuts at:    400     524    1004    1531    2289    2866    3362     400 
   Size:        124     480     527     758     577     496    1144

  Fragments arranged by size: 

                1144     758     577     527     496     480     124
 
BsiEI CG_ry'CG

Cuts at:    104    1648    1694    2902     104 
   Size:       1544      46    1208    1308

  Fragments arranged by size: 

                1544    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    131     142    1120    1898    2088    2880     131 
   Size:         11     978     778     190     792    1357

  Fragments arranged by size: 

                1357     978     792     778     190      11
 
BsmI GAATG_Cn'

Cuts at:    303     320     303 
   Size:         17    4089
 
BsmBI CGTCTCn'nnnn_

Cuts at:    259     259 
   Size:       4106
 
Bsp1286I G_dGCh'C

Cuts at:    131     142     549     600    1120    1731    1824    1898    2088 
   Size:         11     407      51     520     611      93      74     190

Cuts at:   2088    2150    2880     131 
   Size:         62     730    1357

  Fragments arranged by size: 

                1357     730     611     520     407     190      93      74
                  62      51      11
 
BspEI T'CCGG_A

Cuts at:   3326    3326 
   Size:       4106
 
BspHI T'CATG_A

Cuts at:    659    3282     659 
   Size:       2623    1483
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1156    1370    1672    2053    1156 
   Size:        214     302     381    3209

  Fragments arranged by size: 

                3209     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2398    2452    3936    4101      50 
   Size:       2348      54    1484     165      55

  Fragments arranged by size: 

                2348    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    654    2018     654 
   Size:       1364    2742
 
BsrFI r'CCGG_y

Cuts at:   2104    2285    2494    2104 
   Size:        181     209    3716

  Fragments arranged by size: 

                3716     209     181
 
BssSI C'ACGA_G

Cuts at:   2377    2735    2377 
   Size:        358    3748
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1483    1483 
   Size:       4106
 
BstBI TT'CG_AA

Cuts at:   2467    2467 
   Size:       4106
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1018    1018 
   Size:       4106
 
BstYI r'GATC_y

Cuts at:      1     168    1131    1598    1621    1956    2202    3214    3300 
   Size:        167     963     467      23     335     246    1012      86

Cuts at:   3300    3312    3658       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     963     467     449     346     335     246     167
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    731     731 
   Size:       4106
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2056    2237    2400    2056 
   Size:        181     163    3762

  Fragments arranged by size: 

                3762     181     163
 
BtsI GCAGTG_nn'

Cuts at:    155    1349     155 
   Size:       1194    2912
 
DraI TTT'AAA

Cuts at:   1105    1105 
   Size:       4106
 
DrdI GACnn_nn'nnGTC

Cuts at:   1812    2670    1812 
   Size:        858    3248
 
EaeI y'GGCC_r

Cuts at:    833    1691    1865    2256    2283    3628     833 
   Size:        858     174     391      27    1345    1311

  Fragments arranged by size: 

                1345    1311     858     391     174      27
 
EagI C'GGCC_G

Cuts at:   1691    1691 
   Size:       4106
 
EarI CTCTTCn'nnn_

Cuts at:   2129    2339    2129 
   Size:        210    3896
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2314    2624    2770    3916    2314 
   Size:        310     146    1146    2504

  Fragments arranged by size: 

                2504    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1118    1118 
   Size:       4106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    319     489     512     583    1931    2363    3110    3378     319 
   Size:        170      23      71    1348     432     747     268    1047

  Fragments arranged by size: 

                1348    1047     747     432     268     170      71      23
            
 
EcoO109I rG'GnC_Cy

Cuts at:    545     546    1245     545 
   Size:          1     699    3406

  Fragments arranged by size: 

                3406     699       1
 
EcoRI G'AATT_C

Cuts at:   1110    1110 
   Size:       4106
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2125    2125 
   Size:       4106
 
FspI TGC'GCA

Cuts at:   1887    1887 
   Size:       4106
 
HaeII r_GCGC'y

Cuts at:   1540    1788    2810    3789    1540 
   Size:        248    1022     979    1857

  Fragments arranged by size: 

                1857    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    242     274     800     832    1948    1978    1980    2010     242 
   Size:         32     526      32    1116      30       2      30    2338

  Fragments arranged by size: 

                2338    1116     526      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1145    1145 
   Size:       4106
 
KasI G'GCGC_C

Cuts at:   1536    1784    1536 
   Size:        248    3858
 
KpnI G_GTAC'C

Cuts at:   1126    1126 
   Size:       4106
 
MluI A'CGCG_T

Cuts at:   3539    3539 
   Size:       4106
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2777    2961    2777 
   Size:        184    3922
 
MscI TGG'CCA

Cuts at:    835    1867     835 
   Size:       1032    3074
 
MslI CAynn'nnrTG

Cuts at:   2095    2222    2095 
   Size:        127    3979
 
MspA1I CmG'CkG

Cuts at:    561    1004    1217    1531    1891    2904    3149     561 
   Size:        443     213     314     360    1013     245    1518

  Fragments arranged by size: 

                1518    1013     443     360     314     245     213
 
NaeI GCC'GGC

Cuts at:   2287    2287 
   Size:       4106
 
NarI GG'CG_CC

Cuts at:   1537    1785    1537 
   Size:        248    3858
 
NcoI C'CATG_G

Cuts at:    731     731 
   Size:       4106
 
NgoMIV G'CCGG_C

Cuts at:   2285    2285 
   Size:       4106
 
NheI G'CTAG_C

Cuts at:   1075    1075 
   Size:       4106
 
NspI r_CATG'y

Cuts at:    779    1159    1659    2566     779 
   Size:        380     500     907    2319

  Fragments arranged by size: 

                2319     907     500     380
 
PciI A'CATG_T

Cuts at:    775    2562     775 
   Size:       1787    2319
 
PflMI CCAn_nnn'nTGG

Cuts at:    407     606     407 
   Size:        199    3907
 
PmeI GTTT'AAAC

Cuts at:   1105    1105 
   Size:       4106
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3245    3277    3245 
   Size:         32    4074
 
PspOMI G'GGCC_C

Cuts at:    545     545 
   Size:       4106
 
PstI C_TGCA'G

Cuts at:    150    1153    1838     150 
   Size:       1003     685    2418

  Fragments arranged by size: 

                2418    1003     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4106
 
PvuII CAG'CTG

Cuts at:    561    1004    1531    1891     561 
   Size:        443     527     360    2776

  Fragments arranged by size: 

                2776     527     443     360
 
RsrII CG'GwC_CG

Cuts at:   2301    2301 
   Size:       4106
 
SacI G_AGCT'C

Cuts at:   1120    1120 
   Size:       4106
 
SalI G'TCGA_C

Cuts at:   1143    1143 
   Size:       4106
 
SapI GCTCTTCn'nnn_

Cuts at:   2129    2339    2129 
   Size:        210    3896
 
SbfI CC_TGCA'GG

Cuts at:   1153    1153 
   Size:       4106
 
ScaI AGT'ACT

Cuts at:   3623    3623 
   Size:       4106
 
SexAI A'CCwGG_T

Cuts at:    605     605 
   Size:       4106
 
SfcI C'TryA_G

Cuts at:     33     146     891    1149    1834    2827    3018      33 
   Size:        113     745     258     685     993     191    1121

  Fragments arranged by size: 

                1121     993     745     685     258     191     113
 
SfoI GGC'GCC

Cuts at:   1538    1786    1538 
   Size:        248    3858
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4106
 
SmaI CCC'GGG

Cuts at:   1128    1128 
   Size:       4106
 
SmlI C'TyrA_G

Cuts at:   1261    2176    2668    2930    1261 
   Size:        915     492     262    2437

  Fragments arranged by size: 

                2437     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4106
 
SphI G_CATG'C

Cuts at:   1159    1659    1159 
   Size:        500    3606
 
StyI C'CwwG_G

Cuts at:    731    1240    1594     731 
   Size:        509     354    3243

  Fragments arranged by size: 

                3243     509     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1689    1822    2281    2490    1689 
   Size:        133     459     209    3305

  Fragments arranged by size: 

                3305     459     209     133
 
TatI w'GTAC_w

Cuts at:    677     785     922     945    3621     677 
   Size:        108     137      23    2676    1162

  Fragments arranged by size: 

                2676    1162     137     108      23
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    569     601     780     812    3623    3655     569 
   Size:         32     179      32    2811      32    1020

  Fragments arranged by size: 

                2811    1020     179      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1903    1903 
   Size:       4106
 
XbaI T'CTAG_A

Cuts at:   1137    1137 
   Size:       4106
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    1601     114 
   Size:       1487    2619
 
XmaI C'CCGG_G

Cuts at:   1126    1126 
   Size:       4106
 
ZraI GAC'GTC

Cuts at:    253     253 
   Size:       4106
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NspI     PciI    PflMI     PmeI     PpiI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BmgBI
   Bpu10I    BsaXI     BsgI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI
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