Restriction map for
pF1KB5557
(Circular) (Six-base) MAPSORT of: pF1KB5557.seq Check: 5995 from: 1 to: 4505
pF1KB5557 4505 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1543 1543
Size: 4505
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1332 2330 2762 3509 3777 1332
Size: 998 432 747 268 2060
Fragments arranged by size:
2060 998 747 432 268
AfeI AGC'GCT
Cuts at: 1307 1390 1307
Size: 83 4422
AflIII A'CryG_T
Cuts at: 560 586 1857 2961 3938 560
Size: 26 1271 1104 977 1127
Fragments arranged by size:
1271 1127 1104 977 26
AgeI A'CCGG_T
Cuts at: 2893 2893
Size: 4505
AleI CACnn'nnGTG
Cuts at: 1234 1234
Size: 4505
AlwNI CAG_nnn'CTG
Cuts at: 1810 3377 1810
Size: 1567 2938
ApaI G_GGCC'C
Cuts at: 321 321
Size: 4505
ApaLI G'TGCA_C
Cuts at: 3275 3275
Size: 4505
ApoI r'AATT_y
Cuts at: 749 862 1509 3858 4114 749
Size: 113 647 2349 256 1140
Fragments arranged by size:
2349 1140 647 256 113
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 1010 1525 1010
Size: 515 3990
BamHI G'GATC_C
Cuts at: 1530 1530
Size: 4505
BanI G'GyrC_C
Cuts at: 514 1521 1935 2183 2218 514
Size: 1007 414 248 35 2801
Fragments arranged by size:
2801 1007 414 248 35
BanII G_rGCy'C
Cuts at: 118 321 995 1052 1519 2549 118
Size: 203 674 57 467 1030 2074
Fragments arranged by size:
2074 1030 674 467 203 57
BbeI G_GCGC'C
Cuts at: 1939 2187 1939
Size: 248 4257
BbsI GAAGACnn'nnnn_
Cuts at: 233 233
Size: 4505
BciVI GTATCCnnnnn_n'
Cuts at: 325 550 2403 3164 4505 325
Size: 225 1853 761 1341 325
Fragments arranged by size:
1853 1341 761 325 225
BclI T'GATC_A
Cuts at: 2025 2025
Size: 4505
BglI GCCn_nnn'nGGC
Cuts at: 380 380
Size: 4505
BglII A'GATC_T
Cuts at: 2020 4057 2020
Size: 2037 2468
BlpI GC'TnA_GC
Cuts at: 1617 1617
Size: 4505
Bme1580I G_kGCm'C
Cuts at: 321 661 856 2130 2223 3279 321
Size: 340 195 1274 93 1056 1547
Fragments arranged by size:
1547 1274 1056 340 195 93
BmrI ACTGGGnnnn_n'
Cuts at: 1717 1798 2133 4277 1717
Size: 81 335 2144 1945
Fragments arranged by size:
2144 1945 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 918 979 1162 1280 627
Size: 291 61 183 118 3852
Fragments arranged by size:
3852 291 183 118 61
Bpu10I CC'TnA_GC
Cuts at: 1315 1315
Size: 4505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 1467 1681 2560 3052 3350 308
Size: 1159 214 879 492 298 1463
Fragments arranged by size:
1463 1159 879 492 298 214
BsaI GGTCTCn'nnnn_
Cuts at: 800 4209 800
Size: 3409 1096
BsaAI yAC'GTr
Cuts at: 1858 1858
Size: 4505
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 1936 2184 2886 4382 1936
Size: 248 702 1496 2059
Fragments arranged by size:
2059 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1694 2215 2893 3167 3314 3725 1694
Size: 521 678 274 147 411 2474
Fragments arranged by size:
2474 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 541 511
Size: 30 4475
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1123 1123
Size: 4505
BseYI C'CCAG_C
Cuts at: 187 1930 2688 3265 3761 187
Size: 1743 758 577 496 931
Fragments arranged by size:
1743 931 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1079 1082 1079
Size: 3 4502
BsiEI CG_ry'CG
Cuts at: 104 615 2047 2093 3301 104
Size: 511 1432 46 1208 1308
Fragments arranged by size:
1432 1308 1208 511 46
BsiHKAI G_wGCw'C
Cuts at: 280 934 995 1519 2297 2487 3279 280
Size: 654 61 524 778 190 792 1506
Fragments arranged by size:
1506 792 778 654 524 190 61
BsmI GAATG_Cn'
Cuts at: 1193 1193
Size: 4505
Bsp1286I G_dGCh'C
Cuts at: 118 280 321 661 856 934 995 1052 1519
Size: 162 41 340 195 78 61 57 467
Cuts at: 1519 2130 2223 2297 2487 2549 3279 118
Size: 611 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 611 467 340 195 190 162
93 78 74 62 61 57 41
BspEI T'CCGG_A
Cuts at: 3725 3725
Size: 4505
BspHI T'CATG_A
Cuts at: 3681 3681
Size: 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1555 1769 2071 2452 1555
Size: 214 302 381 3608
Fragments arranged by size:
3608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 576 611 2797 2851 4335 4500 50
Size: 526 35 2186 54 1484 165 55
Fragments arranged by size:
2186 1484 526 165 55 54 35
BsrDI GCAATG_nn'
Cuts at: 677 2417 677
Size: 1740 2765
BsrFI r'CCGG_y
Cuts at: 614 1172 2503 2684 2893 614
Size: 558 1331 181 209 2226
Fragments arranged by size:
2226 1331 558 209 181
BssSI C'ACGA_G
Cuts at: 2776 3134 2776
Size: 358 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 1882 1882
Size: 4505
BstBI TT'CG_AA
Cuts at: 2866 2866
Size: 4505
BstYI r'GATC_y
Cuts at: 1 815 1530 1997 2020 2355 2601 3613 3699
Size: 814 715 467 23 335 246 1012 86
Cuts at: 3699 3711 4057 1
Size: 12 346 449
Fragments arranged by size:
1012 814 715 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 271 1232 107
Size: 164 961 3380
Fragments arranged by size:
3380 961 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 477 2455 2636 2799 477
Size: 1978 181 163 2183
Fragments arranged by size:
2183 1978 181 163
BtsI GCAGTG_nn'
Cuts at: 1748 1748
Size: 4505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 873 908 873
Size: 35 4470
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DraIII CAC_nnn'GTG
Cuts at: 1207 1207
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 235 2211 3069 235
Size: 1976 858 1671
Fragments arranged by size:
1976 1671 858
EaeI y'GGCC_r
Cuts at: 612 616 2090 2264 2655 2682 4027 612
Size: 4 1474 174 391 27 1345 1090
Fragments arranged by size:
1474 1345 1090 391 174 27 4
EagI C'GGCC_G
Cuts at: 612 2090 612
Size: 1478 3027
EarI CTCTTCn'nnn_
Cuts at: 405 726 1451 2528 2738 405
Size: 321 725 1077 210 2172
Fragments arranged by size:
2172 1077 725 321 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2713 3023 3169 4315 2713
Size: 310 146 1146 2903
Fragments arranged by size:
2903 1146 310 146
EcoICRI GAG'CTC
Cuts at: 993 1517 993
Size: 524 3981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 918 979 1162 1280 1332 2330 2762 3509
Size: 291 61 183 118 52 998 432 747
Cuts at: 3509 3777 627
Size: 268 1355
Fragments arranged by size:
1355 998 747 432 291 268 183 118
61 52
EcoNI CCTnn'n_nnAGG
Cuts at: 326 326
Size: 4505
EcoO109I rG'GnC_Cy
Cuts at: 317 318 1644 317
Size: 1 1326 3178
Fragments arranged by size:
3178 1326 1
EcoRI G'AATT_C
Cuts at: 749 1509 749
Size: 760 3745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4505
FseI GG_CCGG'CC
Cuts at: 618 618
Size: 4505
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 1309 1392 1939 2187 3209 4188 1309
Size: 83 547 248 1022 979 1626
Fragments arranged by size:
1626 1022 979 547 248 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1278 1310 2347 2377 2379 2409 1278
Size: 32 1037 30 2 30 3374
Fragments arranged by size:
3374 1037 32 30 30 2
HincII GTy'rAC
Cuts at: 1544 1544
Size: 4505
KasI G'GCGC_C
Cuts at: 1935 2183 1935
Size: 248 4257
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 815 1049 3176 3360 815
Size: 234 2127 184 1960
Fragments arranged by size:
2127 1960 234 184
MscI TGG'CCA
Cuts at: 2266 2266
Size: 4505
MslI CAynn'nnrTG
Cuts at: 289 589 1231 1234 2494 2621 289
Size: 300 642 3 1260 127 2173
Fragments arranged by size:
2173 1260 642 300 127 3
MspA1I CmG'CkG
Cuts at: 1096 1616 1930 2290 3303 3548 1096
Size: 520 314 360 1013 245 2053
Fragments arranged by size:
2053 1013 520 360 314 245
NaeI GCC'GGC
Cuts at: 616 2686 616
Size: 2070 2435
NarI GG'CG_CC
Cuts at: 1936 2184 1936
Size: 248 4257
NcoI C'CATG_G
Cuts at: 107 107
Size: 4505
NgoMIV G'CCGG_C
Cuts at: 614 2684 614
Size: 2070 2435
NspI r_CATG'y
Cuts at: 590 1558 2058 2965 590
Size: 968 500 907 2130
Fragments arranged by size:
2130 968 907 500
PasI CC'CwG_GG
Cuts at: 348 348
Size: 4505
PciI A'CATG_T
Cuts at: 586 2961 586
Size: 2375 2130
PflMI CCAn_nnn'nTGG
Cuts at: 490 916 490
Size: 426 4079
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4473
PspOMI G'GGCC_C
Cuts at: 317 317
Size: 4505
PstI C_TGCA'G
Cuts at: 316 1196 1313 1552 2237 316
Size: 880 117 239 685 2584
Fragments arranged by size:
2584 880 685 239 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 1096 1930 2290 1096
Size: 834 360 3311
Fragments arranged by size:
3311 834 360
RsrII CG'GwC_CG
Cuts at: 578 2700 578
Size: 2122 2383
SacI G_AGCT'C
Cuts at: 995 1519 995
Size: 524 3981
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 405 2528 2738 405
Size: 2123 210 2172
Fragments arranged by size:
2172 2123 210
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 4022 4022
Size: 4505
SexAI A'CCwGG_T
Cuts at: 355 355
Size: 4505
SfcI C'TryA_G
Cuts at: 33 312 1192 1309 1548 2233 3226 3417 33
Size: 279 880 117 239 685 993 191 1121
Fragments arranged by size:
1121 993 880 685 279 239 191 117
SfoI GGC'GCC
Cuts at: 1937 2185 1937
Size: 248 4257
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 1527 1527
Size: 4505
SmlI C'TyrA_G
Cuts at: 323 1446 1660 2575 3067 3329 323
Size: 1123 214 915 492 262 1499
Fragments arranged by size:
1499 1123 915 492 262 214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 1558 2058 1558
Size: 500 4005
StuI AGG'CCT
Cuts at: 604 776 604
Size: 172 4333
StyI C'CwwG_G
Cuts at: 107 1639 1993 107
Size: 1532 354 2619
Fragments arranged by size:
2619 1532 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1069 1164 1237 2088 2221 2680 2889 1069
Size: 95 73 851 133 459 209 2685
Fragments arranged by size:
2685 851 459 209 133 95 73
TatI w'GTAC_w
Cuts at: 308 821 4020 308
Size: 513 3199 793
Fragments arranged by size:
3199 793 513
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4022 4054 4022
Size: 32 4473
Tth111I GACn'n_nGTC
Cuts at: 442 2302 442
Size: 1860 2645
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2000 2000
Size: 4505
XmaI C'CCGG_G
Cuts at: 1525 1525
Size: 4505
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI