Restriction map for pF1KB5289
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        AspEI       AvrII       BbeI        BbrPI       
BbvCI       BciVI       BfaI        BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaI        
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KspI        MaeI        MfeI        MluI        MroI        
MslI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        ScaI        SexAI       SfaNI       SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Van91I      XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        358  GGTACC               G^GTACC
AocI          739  CCTAAGG              CC^T[ACGT]AGG
Asp700I       671  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       358  GGTACC               G^GTACC
AspI          379  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         443  TGCGCA               TGC^GCA
BalI          254  TGGCCA               TGG^CCA
BamHI         129  GGATCC               G^GATCC
BbsI          915  GAAGAC               GAAGAC(2/6)
BcgI          224  CGACCAACGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          335  TGATCA               T^GATCA
BglI          262  GCCCTCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         771  AGATCT               A^GATCT
BpmI          666  CTCCAG               CTGGAG(16/14)
BpuAI         915  GAAGAC               GAAGAC(2/6)
BsaBI         766  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         805  ACCGGA               [AT]^CCGG[AT]
BsmI          611  GCATTC               GAATGC(1/-1)
BspHI         894  TCATGA               T^CATGA
BspMI         527  ACCTGC               ACCTGC(4/8)
BsrBI         284  GAGCGG               CCGCTC(-3/-3)
BsrI          545  ACTGG                ACTGG(1/-1)
BstXI         455  CCAGCTTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        739  CCTAAGG              CC^T[ACGT]AGG
EaeI          254  TGGCCA               [CT]^GGCC[GA]
Eco81I        739  CCTAAGG              CC^T[ACGT]AGG
EcoT14I       354  CCATGG               C^C[AT][AT]GG
FbaI          335  TGATCA               T^GATCA
FspI          443  TGCGCA               TGC^GCA
HaeII         111  GGCGCT               [GA]GCGC^[CT]
KpnI          358  GGTACC               GGTAC^C
MaeII         230  ACGT                 A^CGT
MamI          766  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         254  TGGCCA               TGG^CCA
MscI          254  TGGCCA               TGG^CCA
MseI         1169  TTAA                 T^TAA
NcoI          354  CCATGG               C^CATGG
NdeI          482  CATATG               CA^TATG
PaeR7I        682  CTCGAG               C^TCGAG
PflFI         379  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         719  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          894  TCATGA               T^CATGA
SacI          370  GAGCTC               GAGCT^C
SbfI          984  CCTGCAGG             CCTGCA^GG
SfiI          261  GGCCCTCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      984  CCTGCAGG             CCTGCA^GG
StyI          354  CCATGG               C^C[AT][AT]GG
TaqI          683  TCGA                 T^CGA
Tru9I        1169  TTAA                 T^TAA
Tth111I       379  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       683  TCGA                 T^CGA
XhoI          682  CTCGAG               C^TCGAG
XmnI          671  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          593  AAATTC               [GA]^AATT[CT]
              852  GAATTT              
ApaI         1211  GGGCCC               GGGCC^C
             1248  GGGCCC              
ApoI          593  AAATTC               [GA]^AATT[CT]
              852  GAATTT              
AspHI         232  GTGCTC               G[AT]GC[AT]^C
              370  GAGCTC              
AvaI           40  CTCGGG               C^[CT]CG[GA]G
              682  CTCGAG              
BanI          358  GGTACC               G^G[CT][GA]CC
              465  GGCACC              
BsgI          550  GTGCAG               GTGCAG(16/14)
              922  GTGCAG              
BsiHKAI       232  GTGCTC               G[AT]GC[AT]^C
              370  GAGCTC              
BsmAI         172  GTCTC                GTCTC(1/5)
              240  GTCTC               
BsoBI          40  CTCGGG               C^[CT]CG[GA]G
              682  CTCGAG              
BstYI         129  GGATCC               [GA]^GATC[CT]
              771  AGATCT              
DsaI          257  CCACGG               C^C[GA][CT]GG
              354  CCATGG              
EarI         1219  CTCTTC               CTCTTC(1/4)
              703  GAAGAG              
FauI         1152  CCCGC                CCCGC(4/6)
             1105  GCGGG               
Ksp632I      1219  CTCTTC               CTCTTC(1/4)
              703  GAAGAG              
MflI          129  GGATCC               [GA]^GATC[CT]
              771  AGATCT              
MwoI          262  GCCCTCTCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              512  GCAACCCTCGC         
PstI          985  CTGCAG               CTGCA^G
             1129  CTGCAG              
SfcI          985  CTGCAG               C^T[GA][CT]AG
             1129  CTGCAG              
SmlI          406  CTCAAG               C^T[CT][GA]AG
              682  CTCGAG              
Tsp509I       594  AATT                 ^AATT
              853  AATT                
XhoII         129  GGATCC               [GA]^GATC[CT]
              771  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        NciI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       TfiI        
TseI        Tsp45I      TspRI       
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