Restriction map for pF1KB5289
 (Linear) (Six-base) MAPSORT of: KIBB5289.seq  Check: 7511  from: 1  to: 1254



KIBB5289.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

Acc65I G'GTAC_C

Cuts at:      0     358    1254 
   Size:        358     896
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     934     970    1254 
   Size:        934      36     284

  Fragments arranged by size: 

                 934     284      36
 
AlwNI CAG_nnn'CTG

Cuts at:      0       9      53     169    1254 
   Size:          9      44     116    1085

  Fragments arranged by size: 

                1085     116      44       9
 
ApaI G_GGCC'C

Cuts at:      0    1215    1252    1254 
   Size:       1215      37       2

  Fragments arranged by size: 

                1215      37       2
 
ApoI r'AATT_y

Cuts at:      0     593     852    1254 
   Size:        593     259     402

  Fragments arranged by size: 

                 593     402     259
 
AvaI C'yCGr_G

Cuts at:      0      40     682    1254 
   Size:         40     642     572

  Fragments arranged by size: 

                 642     572      40
 
BamHI G'GATC_C

Cuts at:      0     129    1254 
   Size:        129    1125
 
BanI G'GyrC_C

Cuts at:      0     358     465    1254 
   Size:        358     107     789

  Fragments arranged by size: 

                 789     358     107
 
BanII G_rGCy'C

Cuts at:      0      36      88     220     321     374    1215    1252    1254 
   Size:         36      52     132     101      53     841      37       2

  Fragments arranged by size: 

                 841     132     101      53      52      37      36       2
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     922    1254 
   Size:        922     332
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     213     247    1254 
   Size:        213      34    1007

  Fragments arranged by size: 

                1007     213      34
 
BclI T'GATC_A

Cuts at:      0     335    1254 
   Size:        335     919
 
BglI GCCn_nnn'nGGC

Cuts at:      0     268    1254 
   Size:        268     986
 
BglII A'GATC_T

Cuts at:      0     771    1254 
   Size:        771     483
 
Bme1580I G_kGCm'C

Cuts at:      0      47    1215    1252    1254 
   Size:         47    1168      37       2

  Fragments arranged by size: 

                1168      47      37       2
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     555    1254 
   Size:        555     699
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      55     102    1254 
   Size:         55      47    1152

  Fragments arranged by size: 

                1152      55      47
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     651    1254 
   Size:        651     603
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391    1254 
   Size:        391     863
 
BsaBI GATnn'nnATC

Cuts at:      0     770    1254 
   Size:        770     484
 
BsaWI w'CCGG_w

Cuts at:      0     805    1254 
   Size:        805     449
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      10      28      94     136     139     233     883    1254 
   Size:         10      18      66      42       3      94     650     371

  Fragments arranged by size: 

                 650     371      94      66      42      18      10       3
            
 
BseYI C'CCAG_C

Cuts at:      0     279     791    1117    1254 
   Size:        279     512     326     137

  Fragments arranged by size: 

                 512     326     279     137
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     943    1254 
   Size:        571     372     311

  Fragments arranged by size: 

                 571     372     311
 
BsiHKAI G_wGCw'C

Cuts at:      0     236     374    1254 
   Size:        236     138     880

  Fragments arranged by size: 

                 880     236     138
 
BsmI GAATG_Cn'

Cuts at:      0     611    1254 
   Size:        611     643
 
Bsp1286I G_dGCh'C

Cuts at:      0      36      47      88     220     236     321     374    1215 
   Size:         36      11      41     132      16      85      53     841

Cuts at:   1215    1252    1254 
   Size:         37       2

  Fragments arranged by size: 

                 841     132      85      53      41      37      36      16
                  11       2
 
BspHI T'CATG_A

Cuts at:      0     894    1254 
   Size:        894     360
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     536    1254 
   Size:        536     718
 
BsrBI CCG'CTC

Cuts at:      0     286    1254 
   Size:        286     968
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     462    1254 
   Size:        462     792
 
BstYI r'GATC_y

Cuts at:      0     129     771    1254 
   Size:        129     642     483

  Fragments arranged by size: 

                 642     483     129
 
Bsu36I CC'TnA_GG

Cuts at:      0     740    1254 
   Size:        740     514
 
BtgI C'CryG_G

Cuts at:      0     257     354    1254 
   Size:        257      97     900

  Fragments arranged by size: 

                 900     257      97
 
BtsI GCAGTG_nn'

Cuts at:      0     386     652    1254 
   Size:        386     266     602

  Fragments arranged by size: 

                 602     386     266
 
EaeI y'GGCC_r

Cuts at:      0     254    1254 
   Size:        254    1000
 
EarI CTCTTCn'nnn_

Cuts at:      0     698    1225    1254 
   Size:        698     527      29

  Fragments arranged by size: 

                 698     527      29
 
EcoICRI GAG'CTC

Cuts at:      0     372    1254 
   Size:        372     882
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     651     934     970    1254 
   Size:        651     283      36     284

  Fragments arranged by size: 

                 651     284     283      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     720    1211    1212    1248    1249    1254 
   Size:          6     714     491       1      36       1       5

  Fragments arranged by size: 

                 714     491      36       6       5       1       1
 
FspI TGC'GCA

Cuts at:      0     445    1254 
   Size:        445     809
 
HaeII r_GCGC'y

Cuts at:      0     115    1254 
   Size:        115    1139
 
KpnI G_GTAC'C

Cuts at:      0     362    1254 
   Size:        362     892
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     713    1254 
   Size:        200     513     541

  Fragments arranged by size: 

                 541     513     200
 
MscI TGG'CCA

Cuts at:      0     256    1254 
   Size:        256     998
 
MspA1I CmG'CkG

Cuts at:      0      53     166     779     795    1155    1254 
   Size:         53     113     613      16     360      99

  Fragments arranged by size: 

                 613     360     113      99      53      16
 
NcoI C'CATG_G

Cuts at:      0     354    1254 
   Size:        354     900
 
NdeI CA'TA_TG

Cuts at:      0     483    1254 
   Size:        483     771
 
PasI CC'CwG_GG

Cuts at:      0    1123    1244    1254 
   Size:       1123     121      10

  Fragments arranged by size: 

                1123     121      10
 
PfoI T'CCnGG_A

Cuts at:      0     418    1254 
   Size:        418     836
 
PpuMI rG'GwC_Cy

Cuts at:      0     720    1254 
   Size:        720     534
 
PspOMI G'GGCC_C

Cuts at:      0    1211    1248    1254 
   Size:       1211      37       6

  Fragments arranged by size: 

                1211      37       6
 
PspXI vC'TCGA_Gb

Cuts at:      0     682    1254 
   Size:        682     572
 
PstI C_TGCA'G

Cuts at:      0     989    1133    1254 
   Size:        989     144     121

  Fragments arranged by size: 

                 989     144     121
 
PvuII CAG'CTG

Cuts at:      0      53     779     795    1254 
   Size:         53     726      16     459

  Fragments arranged by size: 

                 726     459      53      16
 
SacI G_AGCT'C

Cuts at:      0     374    1254 
   Size:        374     880
 
SanDI GG'GwC_CC

Cuts at:      0     720    1254 
   Size:        720     534
 
SbfI CC_TGCA'GG

Cuts at:      0     989    1254 
   Size:        989     265
 
SfcI C'TryA_G

Cuts at:      0     985    1129    1254 
   Size:        985     144     125

  Fragments arranged by size: 

                 985     144     125
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     268    1254 
   Size:        268     986
 
SmlI C'TyrA_G

Cuts at:      0     406     682    1254 
   Size:        406     276     572

  Fragments arranged by size: 

                 572     406     276
 
StyI C'CwwG_G

Cuts at:      0     354    1254 
   Size:        354     900
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     537     920    1254 
   Size:        537     383     334

  Fragments arranged by size: 

                 537     383     334
 
Tth111I GACn'n_nGTC

Cuts at:      0     382    1254 
   Size:        382     872
 
XhoI C'TCGA_G

Cuts at:      0     682    1254 
   Size:        682     572
 
XmnI GAAnn'nnTTC

Cuts at:      0     675    1254 
   Size:        675     579
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI     BcgI     BclI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI    BpuEI    BsaBI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
     FspI    HaeII     KpnI     MmeI     MscI   MspA1I     NcoI     NdeI
     PasI     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI
    SanDI     SbfI     SfcI     SfiI     SmlI     StyI    TaqII  Tth111I
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BbvCI    BciVI    BfrBI     BlpI    BmgBI
     BmtI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MslI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI     PsrI     PvuI    RsrII    SacII     SalI     SapI     ScaI
    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI     XbaI     XcmI
     XmaI     ZraI
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