Restriction map for pF1KB6934
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MunI        
MvnI        NarI        NciI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          437  GTAGAC               GT^[AC][GT]AC
AclI          485  AACGTT               AA^CGTT
AcsI          550  GAATTT               [GA]^AATT[CT]
AfaI          178  GTAC                 GT^AC
AflIII        174  ACACGT               A^C[GA][CT]GT
ApoI          550  GAATTT               [GA]^AATT[CT]
AspHI         633  GAGCAC               G[AT]GC[AT]^C
BbvI          128  GCAGC                GCAGC(8/12)
BmyI          633  GAGCAC               G[AGT]GC[ACT]^C
BsaAI         175  CACGTA               [CT]AC^GT[GA]
BsgI           34  CTGCAC               GTGCAG(16/14)
BsiEI          86  CGGCCG               CG[GA][CT]^CG
BsiHKAI       633  GAGCAC               G[AT]GC[AT]^C
BsiYI          82  CCGCCGGCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           82  CCGCCGGCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      633  GAGCAC               G[AGT]GC[ACT]^C
BssKI         104  CCTGG                ^CC[ACGT]GG
BstAPI         69  GCAGTCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         104  CCTGG                CC^[AT]GG
DpnII         309  GATC                 ^GATC
EaeI           86  CGGCCG               [CT]^GGCC[GA]
EagI           86  CGGCCG               C^GGCCG
EarI          577  CTCTTC               CTCTTC(1/4)
EclXI          86  CGGCCG               C^GGCCG
Eco52I         86  CGGCCG               C^GGCCG
EcoRII        104  CCTGG                ^CC[AT]GG
EcoT14I       458  CCAAGG               C^C[AT][AT]GG
FauI           81  CCCGC                CCCGC(4/6)
FseI           87  GGCCGGCC             GGCCGG^CC
Ksp632I       577  CTCTTC               CTCTTC(1/4)
MaeIII        278  GTGAC                ^GT[ACGT]AC
MboI          309  GATC                 ^GATC
MseI          499  TTAA                 T^TAA
MvaI          104  CCTGG                CC^[AT]GG
NdeII         309  GATC                 ^GATC
Psp1406I      485  AACGTT               AA^CGTT
RsaI          178  GTAC                 GT^AC
Sau3AI        309  GATC                 ^GATC
ScrFI         104  CCTGG                CC^[ACGT]GG
StyI          458  CCAAGG               C^C[AT][AT]GG
Tru9I         499  TTAA                 T^TAA
TseI          128  GCAGC                G^C[AT]GC
Tsp45I        278  GTGAC                ^GT[GC]AC
Tsp509I       551  AATT                 ^AATT
TspRI         274  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AluI          113  AGCT                 AG^CT
              349  AGCT                
AlwNI          70  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
               99  CAGTTCCTG           
Asp700I       445  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              558  GAAGGTGTTC          
AvaII         342  GGACC                G^G[AT]CC
              431  GGTCC               
BfaI           13  CTAG                 C^TAG
              607  CTAG                
BsaJI         104  CCTGGG               C^C[ACGT][ACGT]GG
              458  CCAAGG              
BsmAI         206  GAGAC                GTCTC(1/5)
               72  GTCTC               
BsmFI         341  GGGAC                GGGAC(10/14)
                6  GTCCC               
BsrFI          84  GCCGGC               [GA]^CCGG[CT]
               88  GCCGGC              
Cfr10I         84  GCCGGC               [GA]^CCGG[CT]
               88  GCCGGC              
Cfr13I        342  GGACC                G^G[ACGT]CC
              431  GGTCC               
DdeI          353  CTCAG                C^T[ACGT]AG
              631  CTGAG               
HaeIII         87  GGCC                 GG^CC
               91  GGCC                
HapII          85  CCGG                 C^CGG
               89  CCGG                
HinfI         219  GATTC                G^A[ACGT]TC
              241  GAATC               
HpaII          85  CCGG                 C^CGG
               89  CCGG                
MaeI           13  CTAG                 C^TAG
              607  CTAG                
MaeII         176  ACGT                 A^CGT
              486  ACGT                
MspI           85  CCGG                 C^CGG
               89  CCGG                
NaeI           84  GCCGGC               GCC^GGC
               88  GCCGGC              
NgoMIV         84  GCCGGC               G^CCGGC
               88  GCCGGC              
NlaIV         341  GGGACC               GG[ACGT]^[ACGT]CC
              517  GGAGCC              
Sau96I        342  GGACC                G^G[ACGT]CC
              431  GGTCC               
SfaNI         229  GATGC                GCATC(5/9)
              611  GCATC               
TaqI           17  TCGA                 T^CGA
              392  TCGA                
TfiI          219  GATTC                G^A[AT]TC
              241  GAATC               
TthHB8I        17  TCGA                 T^CGA
              392  TCGA                
XmnI          445  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              558  GAAGGTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        BsrDI       BsrI        BstF5I      Cac8I       
Fnu4HI      FokI        ItaI        MboII       MnlI        
MwoI        NlaIII      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com