Restriction map for pF1KB0457
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       ApoI        AseI        AsnI        Asp700I     
AspEI       AviII       AvrII       BalI        BamHI       
BbsI        BbvCI       BcgI        BciVI       BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraI        Eam1105I    EarI        
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HindIII     HpaI        Ksp632I     MaeI        MamI        
MluI        MluNI       MroI        MscI        NcoI        
NdeI        NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        887  GGTACC               G^GTACC
AocI          714  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1360  AGCGCT               AGC^GCT
ApaI          214  GGGCCC               GGGCC^C
AscI           26  GGCGCGCC             GG^CGCGCC
Asp718I       887  GGTACC               G^GTACC
AspHI        1389  GAGCAC               G[AT]GC[AT]^C
AspI          850  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1292  CCCGGG               C^[CT]CG[GA]G
BbrPI         544  CACGTG               CAC^GTG
BglI         1042  GCCGCGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         544  CACGTG               [CT]AC^GT[GA]
BsaI          937  GAGACC               GGTCTC(1/5)
BsgI          547  GTGCAG               GTGCAG(16/14)
BsiHKAI      1389  GAGCAC               G[AT]GC[AT]^C
BsoBI        1292  CCCGGG               C^[CT]CG[GA]G
BsrBI         195  CCGCTC               CCGCTC(-3/-3)
BsrDI         822  CATTGC               GCAATG(2/0)
BssHII         27  GCGCGC               G^CGCGC
BstF5I        639  GGATG                GGATG(2/0)
BstYI         737  AGATCC               [GA]^GATC[CT]
Bsu36I        714  CCTGAGG              CC^T[ACGT]AGG
DraIII        142  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          847  GACGACATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1360  AGCGCT               AGC^GCT
Eco81I        714  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       953  CCAAGG               C^C[AT][AT]GG
EcoT22I       228  ATGCAT               ATGCA^T
FokI          639  GGATG                GGATG(9/13)
HgaI          179  GACGC                GACGC(5/10)
HincII        247  GTCAAC               GT[CT]^[GA]AC
HindII        247  GTCAAC               GT[CT]^[GA]AC
KpnI          887  GGTACC               GGTAC^C
MfeI         1417  CAATTG               C^AATTG
MflI          737  AGATCC               [GA]^GATC[CT]
MunI         1417  CAATTG               C^AATTG
NaeI          166  GCCGGC               GCC^GGC
NgoMIV        166  GCCGGC               G^CCGGC
NsiI          228  ATGCAT               ATGCA^T
PflFI         850  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1339  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         544  CACGTG               CAC^GTG
PmlI          544  CACGTG               CAC^GTG
SfiI         1041  GGCCGCGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1292  CCCGGG               CCC^GGG
StyI          953  CCAAGG               C^C[AT][AT]GG
Tsp509I      1418  AATT                 ^AATT
Tth111I       850  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1339  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         737  AGATCC               [GA]^GATC[CT]
XmaCI        1292  CCCGGG               C^CCGGG
XmaI         1292  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI         1117  GGCGCC               G[GA]^CG[CT]C
             1220  GGCGCC              
AfaI          888  GTAC                 GT^AC
             1164  GTAC                
AvaII         801  GGACC                G^G[AT]CC
             1314  GGACC               
BbeI         1117  GGCGCC               GGCGC^C
             1220  GGCGCC              
BclI          923  TGATCA               T^GATCA
             1154  TGATCA              
BsaHI        1117  GGCGCC               G[GA]^CG[CT]C
             1220  GGCGCC              
BsiEI          66  CGGCCG               CG[GA][CT]^CG
              998  CGGCCG              
BsmI         1011  GAATGC               GAATGC(1/-1)
              743  GCATTC              
BsrFI         166  GCCGGC               [GA]^CCGG[CT]
             1393  ACCGGC              
Cfr10I        166  GCCGGC               [GA]^CCGG[CT]
             1393  ACCGGC              
EagI           66  CGGCCG               C^GGCCG
              998  CGGCCG              
EclXI          66  CGGCCG               C^GGCCG
              998  CGGCCG              
Eco52I         66  CGGCCG               C^GGCCG
              998  CGGCCG              
FbaI          923  TGATCA               T^GATCA
             1154  TGATCA              
Hin1I        1117  GGCGCC               G[GA]^CG[CT]C
             1220  GGCGCC              
HinfI         480  GACTC                G^A[ACGT]TC
              880  GAGTC               
KasI         1117  GGCGCC               G^GCGCC
             1220  GGCGCC              
MaeIII        856  GTCAC                ^GT[ACGT]AC
             1342  GTGAC               
MboII         573  GAAGA                GAAGA(8/7)
              633  GAAGA               
MseI          539  TTAA                 T^TAA
              833  TTAA                
MslI          234  CACCACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              768  CACCTCCGTG          
NarI         1117  GGCGCC               GG^CGCC
             1220  GGCGCC              
PleI          480  GACTC                GAGTC(4/5)
              880  GAGTC               
PpuMI         800  GGGACCT              [GA]G^G[AT]CC[CT]
             1313  GGGACCT             
RsaI          888  GTAC                 GT^AC
             1164  GTAC                
SfoI         1117  GGCGCC               GGC^GCC
             1220  GGCGCC              
Tru9I         539  TTAA                 T^TAA
              833  TTAA                
Tsp45I        856  GTCAC                ^GT[GC]AC
             1342  GTGAC               
XcmI         1133  CCAAGCAGGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1184  CCAAGCTGGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstAPI      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
KspI        MaeII       MboI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PstI        PvuII       
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TseI        TspRI       TthHB8I     
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