Restriction map for pF1KB0457
 (Linear) (Six-base) MAPSORT of: KIBB0457.seq  Check: 3019  from: 1  to: 1515



KIBB0457.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1438    1515 
   Size:       1438      77
 
Acc65I G'GTAC_C

Cuts at:      0     887    1515 
   Size:        887     628
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     652    1515 
   Size:         18     634     863

  Fragments arranged by size: 

                 863     634      18
 
AfeI AGC'GCT

Cuts at:      0    1362    1515 
   Size:       1362     153
 
AleI CACnn'nnGTG

Cuts at:      0     238     772    1515 
   Size:        238     534     743

  Fragments arranged by size: 

                 743     534     238
 
AlwNI CAG_nnn'CTG

Cuts at:      0     993    1345    1355    1502    1515 
   Size:        993     352      10     147      13

  Fragments arranged by size: 

                 993     352     147      13      10
 
ApaI G_GGCC'C

Cuts at:      0     218    1515 
   Size:        218    1297
 
AscI GG'CGCG_CC

Cuts at:      0      27    1515 
   Size:         27    1488
 
AvaI C'yCGr_G

Cuts at:      0    1292    1515 
   Size:       1292     223
 
BanI G'GyrC_C

Cuts at:      0     887    1117    1220    1515 
   Size:        887     230     103     295

  Fragments arranged by size: 

                 887     295     230     103
 
BanII G_rGCy'C

Cuts at:      0     218     458    1459    1515 
   Size:        218     240    1001      56

  Fragments arranged by size: 

                1001     240     218      56
 
BbeI G_GCGC'C

Cuts at:      0    1121    1224    1515 
   Size:       1121     103     291

  Fragments arranged by size: 

                1121     291     103
 
BclI T'GATC_A

Cuts at:      0     923    1154    1515 
   Size:        923     231     361

  Fragments arranged by size: 

                 923     361     231
 
BfrBI ATG'CAT

Cuts at:      0     230    1515 
   Size:        230    1285
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1048    1515 
   Size:       1048     467
 
Bme1580I G_kGCm'C

Cuts at:      0     218    1515 
   Size:        218    1297
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     212    1047    1515 
   Size:        212     835     468

  Fragments arranged by size: 

                 835     468     212
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     400     421     472     580     694     733    1057 
   Size:        298     102      21      51     108     114      39     324

Cuts at:   1057    1132    1249    1515 
   Size:         75     117     266

  Fragments arranged by size: 

                 324     298     266     117     114     108     102      75
                  51      39      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     931    1515 
   Size:        931     584
 
BsaAI yAC'GTr

Cuts at:      0     546    1515 
   Size:        546     969
 
BsaHI Gr'CG_yC

Cuts at:      0    1118    1221    1515 
   Size:       1118     103     294

  Fragments arranged by size: 

                1118     294     103
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475     484     514    1515 
   Size:        445      30       9      30    1001

  Fragments arranged by size: 

                1001     445      30      30       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     328     598     601     745     748     919     973 
   Size:        250      78     270       3     144       3     171      54

Cuts at:    973    1252    1515 
   Size:        279     263

  Fragments arranged by size: 

                 279     270     263     250     171     144      78      54
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0    1188    1515 
   Size:       1188     327
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568    1515 
   Size:        568     947
 
BsiEI CG_ry'CG

Cuts at:      0      69    1001    1515 
   Size:         69     932     514

  Fragments arranged by size: 

                 932     514      69
 
BsiHKAI G_wGCw'C

Cuts at:      0    1393    1515 
   Size:       1393     122
 
BsmI GAATG_Cn'

Cuts at:      0     743    1017    1515 
   Size:        743     274     498

  Fragments arranged by size: 

                 743     498     274
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     458    1393    1459    1515 
   Size:        218     240     935      66      56

  Fragments arranged by size: 

                 935     240     218      66      56
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      13     335    1438    1515 
   Size:         13     322    1103      77

  Fragments arranged by size: 

                1103     322      77      13
 
BsrBI CCG'CTC

Cuts at:      0     197    1515 
   Size:        197    1318
 
BsrDI GCAATG_nn'

Cuts at:      0     821    1515 
   Size:        821     694
 
BsrFI r'CCGG_y

Cuts at:      0     166    1393    1515 
   Size:        166    1227     122

  Fragments arranged by size: 

                1227     166     122
 
BssHII G'CGCG_C

Cuts at:      0      27    1515 
   Size:         27    1488
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     107     555    1355    1502    1515 
   Size:        107     448     800     147      13

  Fragments arranged by size: 

                 800     448     147     107      13
 
BstYI r'GATC_y

Cuts at:      0     737    1515 
   Size:        737     778
 
Bsu36I CC'TnA_GG

Cuts at:      0     715    1515 
   Size:        715     800
 
BtgI C'CryG_G

Cuts at:      0      58      96     114     773     917    1043    1515 
   Size:         58      38      18     659     144     126     472

  Fragments arranged by size: 

                 659     472     144     126      58      38      18
 
BtsI GCAGTG_nn'

Cuts at:      0     304    1356    1515 
   Size:        304    1052     159

  Fragments arranged by size: 

                1052     304     159
 
DraIII CAC_nnn'GTG

Cuts at:      0     147    1515 
   Size:        147    1368
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     853    1515 
   Size:        853     662
 
EaeI y'GGCC_r

Cuts at:      0      66     998    1103    1336    1413    1515 
   Size:         66     932     105     233      77     102

  Fragments arranged by size: 

                 932     233     105     102      77      66
 
EagI C'GGCC_G

Cuts at:      0      66     998    1515 
   Size:         66     932     517

  Fragments arranged by size: 

                 932     517      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     298     400     421     472     580     652     694 
   Size:         18     280     102      21      51     108      72      42

Cuts at:    694     733    1057    1132    1249    1515 
   Size:         39     324      75     117     266

  Fragments arranged by size: 

                 324     280     266     117     108     102      75      72
                  51      42      39      21      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     463     697    1126    1228    1515 
   Size:        463     234     429     102     287

  Fragments arranged by size: 

                 463     429     287     234     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0     690     801    1314    1515 
   Size:        690     111     513     201

  Fragments arranged by size: 

                 690     513     201     111
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     390    1515 
   Size:        390    1125
 
HaeII r_GCGC'y

Cuts at:      0    1121    1224    1364    1515 
   Size:       1121     103     140     151

  Fragments arranged by size: 

                1121     151     140     103
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     865     897    1515 
   Size:        865      32     618

  Fragments arranged by size: 

                 865     618      32
 
HincII GTy'rAC

Cuts at:      0     249    1515 
   Size:        249    1266
 
KasI G'GCGC_C

Cuts at:      0    1117    1220    1515 
   Size:       1117     103     295

  Fragments arranged by size: 

                1117     295     103
 
KpnI G_GTAC'C

Cuts at:      0     891    1515 
   Size:        891     624
 
MfeI C'AATT_G

Cuts at:      0    1417    1515 
   Size:       1417      98
 
MslI CAynn'nnrTG

Cuts at:      0     238     772    1515 
   Size:        238     534     743

  Fragments arranged by size: 

                 743     534     238
 
MspA1I CmG'CkG

Cuts at:      0      38      60      71     116     518     610    1045    1094 
   Size:         38      22      11      45     402      92     435      49

Cuts at:   1094    1502    1515 
   Size:        408      13

  Fragments arranged by size: 

                 435     408     402      92      49      45      38      22
                  13      11
 
NaeI GCC'GGC

Cuts at:      0     168    1515 
   Size:        168    1347
 
NarI GG'CG_CC

Cuts at:      0    1118    1221    1515 
   Size:       1118     103     294

  Fragments arranged by size: 

                1118     294     103
 
NgoMIV G'CCGG_C

Cuts at:      0     166    1515 
   Size:        166    1349
 
NsiI A_TGCA'T

Cuts at:      0     232    1515 
   Size:        232    1283
 
PasI CC'CwG_GG

Cuts at:      0    1476    1515 
   Size:       1476      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1345    1515 
   Size:       1345     170
 
PfoI T'CCnGG_A

Cuts at:      0     377     698    1515 
   Size:        377     321     817

  Fragments arranged by size: 

                 817     377     321
 
PmlI CAC'GTG

Cuts at:      0     546    1515 
   Size:        546     969
 
PpuMI rG'GwC_Cy

Cuts at:      0     801    1314    1515 
   Size:        801     513     201

  Fragments arranged by size: 

                 801     513     201
 
PspOMI G'GGCC_C

Cuts at:      0     214    1515 
   Size:        214    1301
 
PstI C_TGCA'G

Cuts at:      0     416    1127    1360    1515 
   Size:        416     711     233     155

  Fragments arranged by size: 

                 711     416     233     155
 
PvuII CAG'CTG

Cuts at:      0      38     610    1502    1515 
   Size:         38     572     892      13

  Fragments arranged by size: 

                 892     572      38      13
 
SacII CC_GC'GG

Cuts at:      0      61     117    1046    1515 
   Size:         61      56     929     469

  Fragments arranged by size: 

                 929     469      61      56
 
SfcI C'TryA_G

Cuts at:      0     412    1123    1215    1356    1515 
   Size:        412     711      92     141     159

  Fragments arranged by size: 

                 711     412     159     141      92
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1048    1515 
   Size:       1048     467
 
SfoI GGC'GCC

Cuts at:      0    1119    1222    1515 
   Size:       1119     103     293

  Fragments arranged by size: 

                1119     293     103
 
SmaI CCC'GGG

Cuts at:      0    1294    1515 
   Size:       1294     221
 
StyI C'CwwG_G

Cuts at:      0     953    1515 
   Size:        953     562
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     234    1468    1515 
   Size:        234    1234      47

  Fragments arranged by size: 

                1234     234      47
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     484     516    1515 
   Size:        484      32     999

  Fragments arranged by size: 

                 999     484      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     853    1515 
   Size:        853     662
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1140    1191    1515 
   Size:       1140      51     324

  Fragments arranged by size: 

                1140     324      51
 
XmaI C'CCGG_G

Cuts at:      0    1292    1515 
   Size:       1292     223
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AleI    AlwNI     ApaI     AscI
     AvaI     BanI    BanII     BbeI     BclI    BfrBI     BglI Bme1580I
     BmrI     BpmI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BssHII   BstAPI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     DrdI     EaeI     EagI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NsiI     PasI    PflMI     PfoI     PmlI    PpuMI
   PspOMI     PstI    PvuII    SacII     SfcI     SfiI     SfoI     SmaI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI    ApaLI     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaWI    BsiWI    BsmBI    BspEI
    BspHI    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     MluI     MmeI     MscI
     NcoI     NdeI     NheI     NotI     NruI     NspI     PacI     PciI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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