Restriction map for pF1KB0413
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BbrPI       BbsI        BbvCI       
BciVI       BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaWI       BseAI       BsiHKAI     BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HincII      HindII      HindIII     HpaI        HphI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1027  GTCTAC               GT^[AC][GT]AC
AcsI          575  AAATTT               [GA]^AATT[CT]
ApaI         1281  GGGCCC               GGGCC^C
ApoI          575  AAATTT               [GA]^AATT[CT]
AvaI          885  CTCGGG               C^[CT]CG[GA]G
BalI           45  TGGCCA               TGG^CCA
BamHI         426  GGATCC               G^GATCC
BanII        1281  GGGCCC               G[GA]GC[CT]^C
BclI          437  TGATCA               T^GATCA
BfaI           91  CTAG                 C^TAG
BsaI          240  GGTCTC               GGTCTC(1/5)
BseRI        1225  GAGGAG               GAGGAG(10/8)
BsmI          518  GCATTC               GAATGC(1/-1)
BsoBI         885  CTCGGG               C^[CT]CG[GA]G
BstYI         426  GGATCC               [GA]^GATC[CT]
EagI          168  CGGCCG               C^GGCCG
EarI          223  CTCTTC               CTCTTC(1/4)
EclXI         168  CGGCCG               C^GGCCG
Eco52I        168  CGGCCG               C^GGCCG
FbaI          437  TGATCA               T^GATCA
HinfI         648  GATTC                G^A[ACGT]TC
Ksp632I       223  CTCTTC               CTCTTC(1/4)
MaeI           91  CTAG                 C^TAG
MaeII         349  ACGT                 A^CGT
MflI          426  GGATCC               [GA]^GATC[CT]
MluNI          45  TGGCCA               TGG^CCA
MscI           45  TGGCCA               TGG^CCA
MseI          995  TTAA                 T^TAA
NaeI         1184  GCCGGC               GCC^GGC
NcoI          944  CCATGG               C^CATGG
NgoMIV       1184  GCCGGC               G^CCGGC
NheI           90  GCTAGC               G^CTAGC
NspI          566  ACATGC               [GA]CATG^[CT]
PvuI          717  CGATCG               CGAT^CG
PvuII         704  CAGCTG               CAG^CTG
SexAI        1013  ACCAGGT              A^CC[AT]GGT
SfcI         1140  CTACAG               C^T[GA][CT]AG
StuI          623  AGGCCT               AGG^CCT
TfiI          648  GATTC                G^A[AT]TC
Tru9I         995  TTAA                 T^TAA
Tsp509I       576  AATT                 ^AATT
XhoII         426  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          138  GTAC                 GT^AC
              351  GTAC                
AvaII         153  GGACC                G^G[AT]CC
             1193  GGACC               
BbeI           80  GGCGCC               GGCGC^C
              202  GGCGCC              
BcgI          177  CGACGACTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              318  GCATGGAGGTCG        
BglI          616  GCCTTCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1370  GCCGCGTGGGC         
BsgI          103  CTGCAC               GTGCAG(16/14)
              677  GTGCAG              
BsiEI         168  CGGCCG               CG[GA][CT]^CG
              717  CGATCG              
BsrFI         134  GCCGGT               [GA]^CCGG[CT]
             1184  GCCGGC              
Cfr10I        134  GCCGGT               [GA]^CCGG[CT]
             1184  GCCGGC              
DdeI          629  CTGAG                C^T[ACGT]AG
             1200  CTCAG               
EcoT22I       316  ATGCAT               ATGCA^T
              568  ATGCAT              
FauI          111  GCGGG                CCCGC(4/6)
              127  GCGGG               
KasI           80  GGCGCC               G^GCGCC
              202  GGCGCC              
KspI          165  CCGCGG               CCGC^GG
              810  CCGCGG              
NarI           80  GGCGCC               GG^CGCC
              202  GGCGCC              
NsiI          316  ATGCAT               ATGCA^T
              568  ATGCAT              
PflMI         732  CCATTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1271  CCAACTCCTGG         
PpuMI         152  AGGACCT              [GA]G^G[AT]CC[CT]
             1192  GGGACCC             
PshAI         750  GACGCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1387  GACATGGGTC          
RsaI          138  GTAC                 GT^AC
              351  GTAC                
SacII         165  CCGCGG               CCGC^GG
              810  CCGCGG              
SfoI           80  GGCGCC               GGC^GCC
              202  GGCGCC              
Van91I        732  CCATTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1271  CCAACTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       BanI        BbvI        BcnI        BmyI        
BsaHI       BsaJI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       ItaI        MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RcaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        
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