Restriction map for pF1KB0413
 (Linear) (Six-base) MAPSORT of: KIBB0413.seq  Check: 4532  from: 1  to: 1401



KIBB0413.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     116     385    1401 
   Size:        116     269    1016

  Fragments arranged by size: 

                1016     269     116
 
AccI GT'mk_AC

Cuts at:      0    1028    1401 
   Size:       1028     373
 
AleI CACnn'nnGTG

Cuts at:      0    1153    1401 
   Size:       1153     248
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     706     774    1401 
   Size:        156     550      68     627

  Fragments arranged by size: 

                 627     550     156      68
 
ApaI G_GGCC'C

Cuts at:      0    1285    1401 
   Size:       1285     116
 
ApoI r'AATT_y

Cuts at:      0     575    1401 
   Size:        575     826
 
AvaI C'yCGr_G

Cuts at:      0     885    1401 
   Size:        885     516
 
BamHI G'GATC_C

Cuts at:      0     426    1401 
   Size:        426     975
 
BanI G'GyrC_C

Cuts at:      0      61      80     202     535     829    1401 
   Size:         61      19     122     333     294     572

  Fragments arranged by size: 

                 572     333     294     122      61      19
 
BanII G_rGCy'C

Cuts at:      0    1285    1401 
   Size:       1285     116
 
BbeI G_GCGC'C

Cuts at:      0      84     206    1401 
   Size:         84     122    1195

  Fragments arranged by size: 

                1195     122      84
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     166     200     307     341    1401 
   Size:        166      34     107      34    1060

  Fragments arranged by size: 

                1060     166     107      34      34
 
BclI T'GATC_A

Cuts at:      0     437    1401 
   Size:        437     964
 
BfrBI ATG'CAT

Cuts at:      0     318     570    1401 
   Size:        318     252     831

  Fragments arranged by size: 

                 831     318     252
 
BglI GCCn_nnn'nGGC

Cuts at:      0     622    1376    1401 
   Size:        622     754      25

  Fragments arranged by size: 

                 754     622      25
 
Bme1580I G_kGCm'C

Cuts at:      0      66     189     538    1285    1401 
   Size:         66     123     349     747     116

  Fragments arranged by size: 

                 747     349     123     116      66
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      25     363    1401 
   Size:         25     338    1038

  Fragments arranged by size: 

                1038     338      25
 
BmtI G_CTAG'C

Cuts at:      0      94    1401 
   Size:         94    1307
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     246    1401 
   Size:        246    1155
 
BsaHI Gr'CG_yC

Cuts at:      0      81     203     751     819    1325    1361    1401 
   Size:         81     122     548      68     506      36      40

  Fragments arranged by size: 

                 548     506     122      81      68      40      36
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1240    1401 
   Size:       1240     161
 
BseYI C'CCAG_C

Cuts at:      0      65     475    1284    1356    1401 
   Size:         65     410     809      72      45

  Fragments arranged by size: 

                 809     410      72      65      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     698    1401 
   Size:         88     610     703

  Fragments arranged by size: 

                 703     610      88
 
BsiEI CG_ry'CG

Cuts at:      0     171     720    1401 
   Size:        171     549     681

  Fragments arranged by size: 

                 681     549     171
 
BsmI GAATG_Cn'

Cuts at:      0     518    1401 
   Size:        518     883
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     826     893    1222    1401 
   Size:        826      67     329     179

  Fragments arranged by size: 

                 826     329     179      67
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     189     538    1285    1401 
   Size:         66     123     349     747     116

  Fragments arranged by size: 

                 747     349     123     116      66
 
BspHI T'CATG_A

Cuts at:      0     560     651    1058    1401 
   Size:        560      91     407     343

  Fragments arranged by size: 

                 560     407     343      91
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     116     385     782    1401 
   Size:        116     269     397     619

  Fragments arranged by size: 

                 619     397     269     116
 
BsrFI r'CCGG_y

Cuts at:      0     134    1184    1401 
   Size:        134    1050     217

  Fragments arranged by size: 

                1050     217     134
 
BstYI r'GATC_y

Cuts at:      0     426    1401 
   Size:        426     975
 
BtgI C'CryG_G

Cuts at:      0     165     810     944    1401 
   Size:        165     645     134     457

  Fragments arranged by size: 

                 645     457     165     134
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      21    1401 
   Size:         21    1380
 
BtsI GCAGTG_nn'

Cuts at:      0     400     406     870     968     975    1401 
   Size:        400       6     464      98       7     426

  Fragments arranged by size: 

                 464     426     400      98       7       6
 
EaeI y'GGCC_r

Cuts at:      0      45     168    1268    1401 
   Size:         45     123    1100     133

  Fragments arranged by size: 

                1100     133     123      45
 
EagI C'GGCC_G

Cuts at:      0     168    1401 
   Size:        168    1233
 
EarI CTCTTCn'nnn_

Cuts at:      0     229    1401 
   Size:        229    1172
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     532    1401 
   Size:        532     869
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     511    1099    1126    1401 
   Size:        511     588      27     275

  Fragments arranged by size: 

                 588     511     275      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153    1193    1281    1401 
   Size:        153    1040      88     120

  Fragments arranged by size: 

                1040     153     120      88
 
HaeII r_GCGC'y

Cuts at:      0      84     206     491     963    1401 
   Size:         84     122     285     472     438

  Fragments arranged by size: 

                 472     438     285     122      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     314     346     709     741    1401 
   Size:        314      32     363      32     660

  Fragments arranged by size: 

                 660     363     314      32      32
 
KasI G'GCGC_C

Cuts at:      0      80     202    1401 
   Size:         80     122    1199

  Fragments arranged by size: 

                1199     122      80
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     269    1067    1401 
   Size:        269     798     334

  Fragments arranged by size: 

                 798     334     269
 
MscI TGG'CCA

Cuts at:      0      47    1401 
   Size:         47    1354
 
MslI CAynn'nnrTG

Cuts at:      0     565    1102    1153    1401 
   Size:        565     537      51     248

  Fragments arranged by size: 

                 565     537     248      51
 
MspA1I CmG'CkG

Cuts at:      0      39     167     706     812    1401 
   Size:         39     128     539     106     589

  Fragments arranged by size: 

                 589     539     128     106      39
 
NaeI GCC'GGC

Cuts at:      0    1186    1401 
   Size:       1186     215
 
NarI GG'CG_CC

Cuts at:      0      81     203    1401 
   Size:         81     122    1198

  Fragments arranged by size: 

                1198     122      81
 
NcoI C'CATG_G

Cuts at:      0     944    1401 
   Size:        944     457
 
NgoMIV G'CCGG_C

Cuts at:      0    1184    1401 
   Size:       1184     217
 
NheI G'CTAG_C

Cuts at:      0      90    1401 
   Size:         90    1311
 
NsiI A_TGCA'T

Cuts at:      0     320     572    1401 
   Size:        320     252     829

  Fragments arranged by size: 

                 829     320     252
 
NspI r_CATG'y

Cuts at:      0     570    1401 
   Size:        570     831
 
PasI CC'CwG_GG

Cuts at:      0     595    1401 
   Size:        595     806
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     738    1277    1401 
   Size:        738     539     124

  Fragments arranged by size: 

                 738     539     124
 
PfoI T'CCnGG_A

Cuts at:      0     119    1401 
   Size:        119    1282
 
PpuMI rG'GwC_Cy

Cuts at:      0     153    1193    1401 
   Size:        153    1040     208

  Fragments arranged by size: 

                1040     208     153
 
PshAI GACnn'nnGTC

Cuts at:      0     754    1391    1401 
   Size:        754     637      10

  Fragments arranged by size: 

                 754     637      10
 
PspOMI G'GGCC_C

Cuts at:      0    1281    1401 
   Size:       1281     120
 
PvuI CG_AT'CG

Cuts at:      0     720    1401 
   Size:        720     681
 
PvuII CAG'CTG

Cuts at:      0     706    1401 
   Size:        706     695
 
SacII CC_GC'GG

Cuts at:      0     168     813    1401 
   Size:        168     645     588

  Fragments arranged by size: 

                 645     588     168
 
SanDI GG'GwC_CC

Cuts at:      0    1193    1401 
   Size:       1193     208
 
SexAI A'CCwGG_T

Cuts at:      0    1013    1401 
   Size:       1013     388
 
SfcI C'TryA_G

Cuts at:      0    1140    1401 
   Size:       1140     261
 
SfoI GGC'GCC

Cuts at:      0      82     204    1401 
   Size:         82     122    1197

  Fragments arranged by size: 

                1197     122      82
 
StuI AGG'CCT

Cuts at:      0     625    1401 
   Size:        625     776
 
StyI C'CwwG_G

Cuts at:      0     306     666     944    1161    1401 
   Size:        306     360     278     217     240

  Fragments arranged by size: 

                 360     306     278     240     217
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      49     546     805    1401 
   Size:         49     497     259     596

  Fragments arranged by size: 

                 596     497     259      49
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      73     460     705    1401 
   Size:         73     387     245     696

  Fragments arranged by size: 

                 696     387     245      73
 

 Enzymes that do cut:

     AarI     AccI     AleI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BcgI     BclI    BfrBI     BglI Bme1580I
     BmrI     BmtI     BsaI    BsaHI    BseRI    BseYI     BsgI    BsiEI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI    BstYI     BtgI
    BtgZI     BtsI     EaeI     EagI     EarI     EciI    EcoNI EcoO109I
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NsiI     NspI     PasI    PflMI
     PfoI    PpuMI    PshAI   PspOMI     PvuI    PvuII    SacII    SanDI
    SexAI     SfcI     SfoI     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI    BbvCI    BciVI    BglII     BlpI    BmgBI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI  BsiHKAI
    BsiWI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NotI     NruI     PacI     PciI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PstI    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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