Restriction map for pF1KB8561
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AccII       AclI        AcsI        AflII       AflIII      
AocI        ApoI        AscI        AseI        AsnI        
AviII       BbeI        BcgI        BfrI        BglII       
BsiEI       BsiWI       Bsp1407I    BspDI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        EagI        EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MfeI        MluI        
MunI        MvnI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PmeI        PpuMI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII        1819  GACGTC               GACGT^C
Acc65I         84  GGTACC               G^GTACC
AccI         1327  GTCTAC               GT^[AC][GT]AC
AgeI         1953  ACCGGT               A^CCGGT
AhdI          586  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1636  GTGCAC               G^TGCAC
Aor51HI       491  AGCGCT               AGC^GCT
ApaLI        1636  GTGCAC               G^TGCAC
Asp718I        84  GGTACC               G^GTACC
AspEI         586  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          832  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1096  CCTAGG               C^CTAGG
BalI         2873  TGGCCA               TGG^CCA
BamHI         854  GGATCC               G^GATCC
BbrPI         364  CACGTG               CAC^GTG
BbsI          412  GAAGAC               GAAGAC(2/6)
BbvCI         273  GCTGAGG              CCTCAGC(-5/-2)
BclI         1427  TGATCA               T^GATCA
BlnI         1096  CCTAGG               C^CTAGG
BlpI          225  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      225  GCTGAGC              GC^T[ACGT]AGC
BpuAI         412  GAAGAC               GAAGAC(2/6)
BsaBI        1467  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1756  CGTCTC               CGTCTC(1/5)
BsmI         2085  GAATGC               GAATGC(1/-1)
BssSI        1576  CTCGTG               CACGAG(-5/-1)
BstAPI       1625  GCATCAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         225  GCTGAGC              GC^T[ACGT]AGC
DraIII       1453  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1645  GACATTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      586  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      491  AGCGCT               AGC^GCT
FbaI         1427  TGATCA               T^GATCA
HaeII         491  AGCGCT               [GA]GCGC^[CT]
KpnI           84  GGTACC               GGTAC^C
MamI         1467  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2873  TGGCCA               TGG^CCA
MscI         2873  TGGCCA               TGG^CCA
PflFI         832  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1953  ACCGGT               A^CCGGT
PmaCI         364  CACGTG               CAC^GTG
PmlI          364  CACGTG               CAC^GTG
PshAI        1699  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI          954  AGTACT               AGT^ACT
SfiI         2874  GGCCAATCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI          868  ACTAGT               A^CTAGT
StuI          986  AGGCCT               AGG^CCT
Tsp509I      1508  AATT                 ^AATT
Tth111I       832  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

ApaI          241  GGGCCC               GGGCC^C
             2434  GGGCCC              
AvaI         2451  CCCGAG               C^[CT]CG[GA]G
             2581  CCCGAG              
BciVI         116  GTATCC               GTATCC(6/5)
             1512  GTATCC              
BsaAI         364  CACGTG               [CT]AC^GT[GA]
             2590  TACGTG              
BsaI          787  GAGACC               GGTCTC(1/5)
             1922  GAGACC              
BsoBI        2451  CCCGAG               C^[CT]CG[GA]G
             2581  CCCGAG              
BsrBI         228  GAGCGG               CCGCTC(-3/-3)
             1543  GAGCGG              
CfoI          492  GCGC                 GCG^C
             2462  GCGC                
EaeI          896  TGGCCG               [CT]^GGCC[GA]
             2873  TGGCCA              
EarI         1480  GAAGAG               CTCTTC(1/4)
             2245  GAAGAG              
HhaI          492  GCGC                 GCG^C
             2462  GCGC                
HinP1I        492  GCGC                 G^CGC
             2462  GCGC                
Ksp632I      1480  GAAGAG               CTCTTC(1/4)
             2245  GAAGAG              
MseI          664  TTAA                 T^TAA
             1790  TTAA                
NspI          485  ACATGC               [GA]CATG^[CT]
             1841  GCATGT              
SexAI         833  ACCTGGT              A^CC[AT]GGT
             2507  ACCTGGT             
SmlI          121  CTCAAG               C^T[CT][GA]AG
             2554  CTCAAG              
TfiI         1212  GATTC                G^A[AT]TC
             1281  GAATC               
Tru9I         664  TTAA                 T^TAA
             1790  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccIII      AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Asp700I     AspHI       AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspEI       BspHI       BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        Hin1I       HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MroI        MslI        MspA1I      MspI        MvaI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       PflMI       PleI        
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmnI        
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