Restriction map for pF1KB8561
 (Linear) (Six-base) MAPSORT of: KIBB8561.seq  Check: 8120  from: 1  to: 2901



KIBB8561.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     898    2901 
   Size:        898    2003
 
AatII G_ACGT'C

Cuts at:      0    1823    2901 
   Size:       1823    1078
 
AccI GT'mk_AC

Cuts at:      0    1328    2901 
   Size:       1328    1573
 
Acc65I G'GTAC_C

Cuts at:      0      84    2901 
   Size:         84    2817
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     301     451     943    1582    2901 
   Size:         77     224     150     492     639    1319

  Fragments arranged by size: 

                1319     639     492     224     150      77
 
AfeI AGC'GCT

Cuts at:      0     493    2901 
   Size:        493    2408
 
AgeI A'CCGG_T

Cuts at:      0    1953    2901 
   Size:       1953     948
 
AhdI GACnn_n'nnGTC

Cuts at:      0     591    2901 
   Size:        591    2310
 
AleI CACnn'nnGTG

Cuts at:      0    1576    2901 
   Size:       1576    1325
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     541    2901 
   Size:        541    2360
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1540    1572    2901 
   Size:       1540      32    1329

  Fragments arranged by size: 

                1540    1329      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1021    2259    2706    2802    2901 
   Size:       1021    1238     447      96      99

  Fragments arranged by size: 

                1238    1021     447      99      96
 
ApaI G_GGCC'C

Cuts at:      0     245    2438    2901 
   Size:        245    2193     463

  Fragments arranged by size: 

                2193     463     245
 
ApaLI G'TGCA_C

Cuts at:      0    1636    2901 
   Size:       1636    1265
 
AvaI C'yCGr_G

Cuts at:      0    2451    2581    2901 
   Size:       2451     130     320

  Fragments arranged by size: 

                2451     320     130
 
AvrII C'CTAG_G

Cuts at:      0    1096    2901 
   Size:       1096    1805
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      99     132    2901 
   Size:         99      33    2769

  Fragments arranged by size: 

                2769      99      33
 
BamHI G'GATC_C

Cuts at:      0     854    2901 
   Size:        854    2047
 
BanI G'GyrC_C

Cuts at:      0      84     926     976    2695    2901 
   Size:         84     842      50    1719     206

  Fragments arranged by size: 

                1719     842     206      84      50
 
BanII G_rGCy'C

Cuts at:      0      20      74     245     548     608    1490    2120    2438 
   Size:         20      54     171     303      60     882     630     318

Cuts at:   2438    2555    2567    2732    2901 
   Size:        117      12     165     169

  Fragments arranged by size: 

                 882     630     318     303     171     169     165     117
                  60      54      20      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     419    2901 
   Size:        419    2482
 
BbvCI CC'TCA_GC

Cuts at:      0     274    2901 
   Size:        274    2627
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     127    1523    2901 
   Size:        127    1396    1378

  Fragments arranged by size: 

                1396    1378     127
 
BclI T'GATC_A

Cuts at:      0    1427    2901 
   Size:       1427    1474
 
BglI GCCn_nnn'nGGC

Cuts at:      0     985    2454    2881    2901 
   Size:        985    1469     427      20

  Fragments arranged by size: 

                1469     985     427      20
 
BlpI GC'TnA_GC

Cuts at:      0     226    2901 
   Size:        226    2675
 
Bme1580I G_kGCm'C

Cuts at:      0      43     245     981    1640    2438    2698    2901 
   Size:         43     202     736     659     798     260     203

  Fragments arranged by size: 

                 798     736     659     260     203     202      43
 
BmgBI CAC'GTC

Cuts at:      0     111    2901 
   Size:        111    2790
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1991    2901 
   Size:       1991     910
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2105    2901 
   Size:       2105     796
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     958    1171    1561    1639    1906    1933    2443 
   Size:        562     396     213     390      78     267      27     510

Cuts at:   2443    2569    2901 
   Size:        126     332

  Fragments arranged by size: 

                 562     510     396     390     332     267     213     126
                  78      27
 
Bpu10I CC'TnA_GC

Cuts at:      0      22     274     547     721    1855    2338    2901 
   Size:         22     252     273     174    1134     483     563

  Fragments arranged by size: 

                1134     563     483     273     252     174      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106    2539    2901 
   Size:        106    2433     362

  Fragments arranged by size: 

                2433     362     106
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     781    1916    2901 
   Size:        781    1135     985

  Fragments arranged by size: 

                1135     985     781
 
BsaAI yAC'GTr

Cuts at:      0     366    2592    2901 
   Size:        366    2226     309

  Fragments arranged by size: 

                2226     366     309
 
BsaBI GATnn'nnATC

Cuts at:      0    1471    2901 
   Size:       1471    1430
 
BsaHI Gr'CG_yC

Cuts at:      0    1820    2398    2831    2901 
   Size:       1820     578     433      70

  Fragments arranged by size: 

                1820     578     433      70
 
BsaWI w'CCGG_w

Cuts at:      0     251     473     680    1655    1953    2495    2901 
   Size:        251     222     207     975     298     542     406

  Fragments arranged by size: 

                 975     542     406     298     251     222     207
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     718     748    1540    1570    2338    2368    2901 
   Size:        718      30     792      30     768      30     533

  Fragments arranged by size: 

                 792     768     718     533      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     742     745     955    1498    2230    2362    2797    2901 
   Size:        742       3     210     543     732     132     435     104

  Fragments arranged by size: 

                 742     732     543     435     210     132     104       3
            
 
BseYI C'CCAG_C

Cuts at:      0     531     963    1527    1692    1982    2149    2233    2252 
   Size:        531     432     564     165     290     167      84      19

Cuts at:   2252    2457    2901 
   Size:        205     444

  Fragments arranged by size: 

                 564     531     444     432     290     205     167     165
                  84      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     346     361    1371    2049    2901 
   Size:        298      48      15    1010     678     852

  Fragments arranged by size: 

                1010     852     678     298      48      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     548     608    1073    1571    1640    2555    2567    2732 
   Size:        548      60     465     498      69     915      12     165

Cuts at:   2732    2901 
   Size:        169

  Fragments arranged by size: 

                 915     548     498     465     169     165      69      60
                  12
 
BsmI GAATG_Cn'

Cuts at:      0    2091    2901 
   Size:       2091     810
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1762    2901 
   Size:       1762    1139
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      43      74     245     548     608     981    1073 
   Size:         20      23      31     171     303      60     373      92

Cuts at:   1073    1490    1571    1640    2120    2438    2555    2567    2698 
   Size:        417      81      69     480     318     117      12     131

Cuts at:   2698    2732    2901 
   Size:         34     169

  Fragments arranged by size: 

                 480     417     373     318     303     171     169     131
                 117      92      81      69      60      34      31      23
                  20      12
 
BspEI T'CCGG_A

Cuts at:      0     473     680    1655    2901 
   Size:        473     207     975    1246

  Fragments arranged by size: 

                1246     975     473     207
 
BspHI T'CATG_A

Cuts at:      0     740    1412    1430    1970    2901 
   Size:        740     672      18     540     931

  Fragments arranged by size: 

                 931     740     672     540      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     898    1381    2849    2901 
   Size:        898     483    1468      52

  Fragments arranged by size: 

                1468     898     483      52
 
BsrBI CCG'CTC

Cuts at:      0     230    1545    2901 
   Size:        230    1315    1356

  Fragments arranged by size: 

                1356    1315     230
 
BsrDI GCAATG_nn'

Cuts at:      0    1256    1646    2063    2901 
   Size:       1256     390     417     838

  Fragments arranged by size: 

                1256     838     417     390
 
BsrFI r'CCGG_y

Cuts at:      0      50     763    1055    1925    1953    2003    2901 
   Size:         50     713     292     870      28      50     898

  Fragments arranged by size: 

                 898     870     713     292      50      50      28
 
BssSI C'ACGA_G

Cuts at:      0    1576    2901 
   Size:       1576    1325
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1631    2901 
   Size:       1631    1270
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      81     894    2112    2190    2871    2901 
   Size:         81     813    1218      78     681      30

  Fragments arranged by size: 

                1218     813     681      81      78      30
 
BstYI r'GATC_y

Cuts at:      0     197     302     854    1133    1850    2901 
   Size:        197     105     552     279     717    1051

  Fragments arranged by size: 

                1051     717     552     279     197     105
 
BtgI C'CryG_G

Cuts at:      0      74    1607    2069    2366    2901 
   Size:         74    1533     462     297     535

  Fragments arranged by size: 

                1533     535     462     297      74
 
BtsI GCAGTG_nn'

Cuts at:      0    1430    1588    2901 
   Size:       1430     158    1313

  Fragments arranged by size: 

                1430    1313     158
 
DraIII CAC_nnn'GTG

Cuts at:      0    1458    2901 
   Size:       1458    1443
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1651    2901 
   Size:       1651    1250
 
EaeI y'GGCC_r

Cuts at:      0     896    2873    2901 
   Size:        896    1977      28

  Fragments arranged by size: 

                1977     896      28
 
EarI CTCTTCn'nnn_

Cuts at:      0    1475    2240    2901 
   Size:       1475     765     661

  Fragments arranged by size: 

                1475     765     661
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     249     847    2901 
   Size:        249     598    2054

  Fragments arranged by size: 

                2054     598     249
 
EcoICRI GAG'CTC

Cuts at:      0     546     606    2553    2565    2730    2901 
   Size:        546      60    1947      12     165     171

  Fragments arranged by size: 

                1947     546     171     165      60      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     301     451     562     943     958    1171    1561 
   Size:         77     224     150     111     381      15     213     390

Cuts at:   1561    1582    1639    1906    1933    2443    2569    2901 
   Size:         21      57     267      27     510     126     332

  Fragments arranged by size: 

                 510     390     381     332     267     224     213     150
                 126     111      77      57      27      21      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     433     613     720    1015    2779    2901 
   Size:        433     180     107     295    1764     122

  Fragments arranged by size: 

                1764     433     295     180     122     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0      61    2434    2435    2901 
   Size:         61    2373       1     466

  Fragments arranged by size: 

                2373     466      61       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     147     615    1890    2901 
   Size:        147     468    1275    1011

  Fragments arranged by size: 

                1275    1011     468     147
 
HaeII r_GCGC'y

Cuts at:      0     495    2901 
   Size:        495    2406
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     577     609    2338    2370    2901 
   Size:        577      32    1729      32     531

  Fragments arranged by size: 

                1729     577     531      32      32
 
KpnI G_GTAC'C

Cuts at:      0      88    2901 
   Size:         88    2813
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    1821    2253    2901 
   Size:        620    1201     432     648

  Fragments arranged by size: 

                1201     648     620     432
 
MscI TGG'CCA

Cuts at:      0    2875    2901 
   Size:       2875      26
 
MslI CAynn'nnrTG

Cuts at:      0      79     826    1576    1666    1906    2068    2901 
   Size:         79     747     750      90     240     162     833

  Fragments arranged by size: 

                 833     750     747     240     162      90      79
 
MspA1I CmG'CkG

Cuts at:      0     531     797     864     936     963    1979    2259    2574 
   Size:        531     266      67      72      27    1016     280     315

Cuts at:   2574    2901 
   Size:        327

  Fragments arranged by size: 

                1016     531     327     315     280     266      72      67
                  27
 
NaeI GCC'GGC

Cuts at:      0      52     765    1057    2901 
   Size:         52     713     292    1844

  Fragments arranged by size: 

                1844     713     292      52
 
NcoI C'CATG_G

Cuts at:      0      74    1607    2069    2366    2901 
   Size:         74    1533     462     297     535

  Fragments arranged by size: 

                1533     535     462     297      74
 
NgoMIV G'CCGG_C

Cuts at:      0      50     763    1055    2901 
   Size:         50     713     292    1846

  Fragments arranged by size: 

                1846     713     292      50
 
NspI r_CATG'y

Cuts at:      0     489    1845    2901 
   Size:        489    1356    1056

  Fragments arranged by size: 

                1356    1056     489
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1252    1539    1769    2901 
   Size:       1252     287     230    1132

  Fragments arranged by size: 

                1252    1132     287     230
 
PfoI T'CCnGG_A

Cuts at:      0     200    2129    2210    2294    2901 
   Size:        200    1929      81      84     607

  Fragments arranged by size: 

                1929     607     200      84      81
 
PmlI CAC'GTG

Cuts at:      0     366    2901 
   Size:        366    2535
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1540    1572    2901 
   Size:       1540      32    1329

  Fragments arranged by size: 

                1540    1329      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1703    2901 
   Size:       1703    1198
 
PspOMI G'GGCC_C

Cuts at:      0     241    2434    2901 
   Size:        241    2193     467

  Fragments arranged by size: 

                2193     467     241
 
PstI C_TGCA'G

Cuts at:      0      69     530    1064    1186    1595    2207    2898    2901 
   Size:         69     461     534     122     409     612     691       3

  Fragments arranged by size: 

                 691     612     534     461     409     122      69       3
            
 
PvuII CAG'CTG

Cuts at:      0     531     864     963    2259    2574    2901 
   Size:        531     333      99    1296     315     327

  Fragments arranged by size: 

                1296     531     333     327     315      99
 
SacI G_AGCT'C

Cuts at:      0     548     608    2555    2567    2732    2901 
   Size:        548      60    1947      12     165     169

  Fragments arranged by size: 

                1947     548     169     165      60      12
 
ScaI AGT'ACT

Cuts at:      0     956    2901 
   Size:        956    1945
 
SexAI A'CCwGG_T

Cuts at:      0     833    2507    2901 
   Size:        833    1674     394

  Fragments arranged by size: 

                1674     833     394
 
SfcI C'TryA_G

Cuts at:      0      65     135     526    1060    1182    1591    2203    2894 
   Size:         65      70     391     534     122     409     612     691

Cuts at:   2894    2901 
   Size:          7

  Fragments arranged by size: 

                 691     612     534     409     391     122      70      65
                   7
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2881    2901 
   Size:       2881      20
 
SmlI C'TyrA_G

Cuts at:      0     121    2554    2901 
   Size:        121    2433     347

  Fragments arranged by size: 

                2433     347     121
 
SpeI A'CTAG_T

Cuts at:      0     868    2901 
   Size:        868    2033
 
StuI AGG'CCT

Cuts at:      0     988    2901 
   Size:        988    1913
 
StyI C'CwwG_G

Cuts at:      0      74    1074    1096    1607    2069    2366    2864    2901 
   Size:         74    1000      22     511     462     297     498      37

  Fragments arranged by size: 

                1000     511     498     462     297      74      37      22
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     322     332    2157    2713    2901 
   Size:        322      10    1825     556     188

  Fragments arranged by size: 

                1825     556     322     188      10
 
TatI w'GTAC_w

Cuts at:      0     954    1583    2162    2901 
   Size:        954     629     579     739

  Fragments arranged by size: 

                 954     739     629     579
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     277     309     361     393     718     750    1092    1124 
   Size:        277      32      52      32     325      32     342      32

Cuts at:   1124    2317    2349    2456    2488    2901 
   Size:       1193      32     107      32     413

  Fragments arranged by size: 

                1193     413     342     325     277     107      52      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     835    2901 
   Size:        835    2066
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     252    1146    1614    2487    2901 
   Size:        252     894     468     873     414

  Fragments arranged by size: 

                 894     873     468     414     252
 
XmnI GAAnn'nnTTC

Cuts at:      0     163     748     806    2901 
   Size:        163     585      58    2095

  Fragments arranged by size: 

                2095     585     163      58
 
ZraI GAC'GTC

Cuts at:      0    1821    2901 
   Size:       1821    1080
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI
     BtgI     BtsI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI    PflMI
     PfoI     PmlI     PpiI    PshAI   PspOMI     PstI    PvuII     SacI
     ScaI    SexAI     SfcI     SfiI     SmlI     SpeI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII   AflIII     ApoI     AscI     AseI    AsiSI     BbeI
     BcgI    BfrBI    BglII     BmtI    BsiEI    BsiWI    BsrGI   BssHII
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     EagI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     MluI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI    PpuMI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     SwaI     XbaI     XhoI     XmaI
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