Restriction map for
pF1KB6687
(Circular) (Six-base) MAPSORT of: pF1KB6687.seq Check: 4533 from: 1 to: 4394
pF1KB6687 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 722 4234 722
Size: 3512 882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 2219 2651 3398 3666 1112
Size: 1107 432 747 268 1840
Fragments arranged by size:
1840 1107 747 432 268
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AhdI GACnn_n'nnGTC
Cuts at: 732 732
Size: 4394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 468 468
Size: 4394
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 825 857 825
Size: 32 4362
AlwNI CAG_nnn'CTG
Cuts at: 513 1366 1699 3266 513
Size: 853 333 1567 1641
Fragments arranged by size:
1641 1567 853 333
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 189 406 847 1398 3747 4003 189
Size: 217 441 551 2349 256 580
Fragments arranged by size:
2349 580 551 441 256 217
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 688 1414 688
Size: 726 3668
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 1410 1824 2072 2107 1410
Size: 414 248 35 3697
Fragments arranged by size:
3697 414 248 35
BanII G_rGCy'C
Cuts at: 216 437 1408 2438 216
Size: 221 971 1030 2172
Fragments arranged by size:
2172 1030 971 221
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 1298 1298
Size: 4394
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 761 801 1256 1914 761
Size: 40 455 658 3241
Fragments arranged by size:
3241 658 455 40
BfrBI ATG'CAT
Cuts at: 1024 1024
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 313 985 313
Size: 672 3722
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 277 1506 277
Size: 1229 3165
Bme1580I G_kGCm'C
Cuts at: 2019 2112 3168 2019
Size: 93 1056 3245
Fragments arranged by size:
3245 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 563 1606 1687 2022 4166 563
Size: 1043 81 335 2144 791
Fragments arranged by size:
2144 1043 791 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
Bpu10I CC'TnA_GC
Cuts at: 207 207
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 1570 2449 2941 3239 200
Size: 1370 879 492 298 1355
Fragments arranged by size:
1370 1355 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 446 1908 3945 6
Size: 440 1462 2037 455
Fragments arranged by size:
2037 1462 455 440
BsaHI Gr'CG_yC
Cuts at: 655 1825 2073 2775 4271 655
Size: 1170 248 702 1496 778
Fragments arranged by size:
1496 1170 778 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1213 1213
Size: 4394
BseYI C'CCAG_C
Cuts at: 948 1366 1819 2577 3154 3650 948
Size: 418 453 758 577 496 1692
Fragments arranged by size:
1692 758 577 496 453 418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1268
Size: 4394
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 216 437 687 1408 2186 2376 3168 216
Size: 221 250 721 778 190 792 1442
Fragments arranged by size:
1442 792 778 721 250 221 190
Bsp1286I G_dGCh'C
Cuts at: 216 437 687 1408 2019 2112 2186 2376 2438
Size: 221 250 721 611 93 74 190 62
Cuts at: 2438 3168 216
Size: 730 1442
Fragments arranged by size:
1442 730 721 611 250 221 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 480 851 3570 480
Size: 371 2719 1304
Fragments arranged by size:
2719 1304 371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1444 1658 1960 2341 1444
Size: 214 302 381 3497
Fragments arranged by size:
3497 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 2392 2573 2782 2392
Size: 181 209 4004
Fragments arranged by size:
4004 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 1011 1011
Size: 4394
BstYI r'GATC_y
Cuts at: 1 1034 1115 1320 1419 1886 1909 2244 2490
Size: 1033 81 205 99 467 23 335 246
Cuts at: 2490 3502 3588 3600 3946 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1033 1012 467 449 346 335 246 205
99 86 81 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 1259 1637 1259
Size: 378 4016
DraI TTT'AAA
Cuts at: 194 1393 194
Size: 1199 3195
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 623 1979 2153 2544 2571 3916 623
Size: 1356 174 391 27 1345 1101
Fragments arranged by size:
1356 1345 1101 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 934 1151 2417 2627 934
Size: 217 1266 210 2701
Fragments arranged by size:
2701 1266 217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 214 435 1406 214
Size: 221 971 3202
Fragments arranged by size:
3202 971 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 2219 2651 3398 3666 1112
Size: 1107 432 747 268 1840
Fragments arranged by size:
1840 1107 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1533 1533
Size: 4394
EcoRI G'AATT_C
Cuts at: 847 1398 847
Size: 551 3843
EcoRV GAT'ATC
Cuts at: 184 184
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 231 2175 231
Size: 1944 2450
HaeII r_GCGC'y
Cuts at: 1828 2076 3098 4077 1828
Size: 248 1022 979 2145
Fragments arranged by size:
2145 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 718 746 750 778 985 1017 2236 2266 2268
Size: 28 4 28 207 32 1219 30 2
Cuts at: 2268 2298 718
Size: 30 2814
Fragments arranged by size:
2814 1219 207 32 30 30 28 28
4 2
HincII GTy'rAC
Cuts at: 726 1316 1433 726
Size: 590 117 3687
Fragments arranged by size:
3687 590 117
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1080 1348 3065 3249 1080
Size: 268 1717 184 2225
Fragments arranged by size:
2225 1717 268 184
MscI TGG'CCA
Cuts at: 625 2155 625
Size: 1530 2864
MslI CAynn'nnrTG
Cuts at: 427 2383 2510 427
Size: 1956 127 2311
Fragments arranged by size:
2311 1956 127
MspA1I CmG'CkG
Cuts at: 952 1366 1505 1819 2179 3192 3437 952
Size: 414 139 314 360 1013 245 1909
Fragments arranged by size:
1909 1013 414 360 314 245 139
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NsiI A_TGCA'T
Cuts at: 1026 1026
Size: 4394
NspI r_CATG'y
Cuts at: 1447 1947 2854 1447
Size: 500 907 2987
Fragments arranged by size:
2987 907 500
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 314 346 3533 3565 314
Size: 32 3187 32 1143
Fragments arranged by size:
3187 1143 32 32
PshAI GACnn'nnGTC
Cuts at: 617 1349 617
Size: 732 3662
PstI C_TGCA'G
Cuts at: 636 1441 2126 636
Size: 805 685 2904
Fragments arranged by size:
2904 805 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 952 1366 1819 2179 952
Size: 414 453 360 3167
Fragments arranged by size:
3167 453 414 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 216 437 1408 216
Size: 221 971 3202
Fragments arranged by size:
3202 971 221
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 200 318 632 678 1040 1383 1437 2122
Size: 167 118 314 46 362 343 54 685
Cuts at: 2122 3115 3306 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 362 343 314 191 167
118 54 46
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 215 1549 2464 2956 3218 215
Size: 1334 915 492 262 1391
Fragments arranged by size:
1391 1334 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 699 1528 1882 699
Size: 829 354 3211
Fragments arranged by size:
3211 829 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 731 1977 2110 2569 2778 607
Size: 124 1246 133 459 209 2223
Fragments arranged by size:
2223 1246 459 209 133 124
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1100 1132 3911 3943 1100
Size: 32 2779 32 1551
Fragments arranged by size:
2779 1551 32 32
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1353 1425 1353
Size: 72 4322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
XmnI GAAnn'nnTTC
Cuts at: 379 407 928 379
Size: 28 521 3845
Fragments arranged by size:
3845 521 28
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI ApaI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BpmI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI